Q604B7 (CYSD_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	MCA2629

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340205

Sequences

	Sequence		Length	Mass (Da)
	Q604B7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: CED721D7475F85F2	FASTA	302	35,358
	10 20 30 40 50 60 MNAHKLTHLK QLESESIHIF REVVSEFERP VMLYSIGKDS AVMLHLAMKA FYPGKPPFPL 70 80 90 100 110 120 MHVDTTWKFR DMIAFRDRRA QELGLDLIIH VNEEGVRQGI NPFVHGSKKH TDIMKTEGLK 130 140 150 160 170 180 QALDKYRFDA AFGGARRDEE KSRAKERVYS FRDRHHRWDP KNQRPELWNL YNGKINKGES 190 200 210 220 230 240 IRVFPLSNWT ELDVWQYIYL EDIPIVPLYF AAERPVVERD GMLIMVDDER MPLLPGEVPM 250 260 270 280 290 300 RKKVRFRTLG CYPLTGAIES DAATLPEIIQ EMLLTRTSER QGRLIDHDQA GSMEEKKKEG YF

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU91270.1.
RefSeq	YP_115033.1. NC_002977.6.
3D structure databases
HSSP	HSSP built from PDB template 1ZUN based on UniProtKB Q87WW0.
ProteinModelPortal	Q604B7.
SMR	Q604B7. Positions 5-212.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3104542.
GenomeReviews	Gene locus MCA2629 in contig AE017282_GR.
KEGG	mca:MCA2629.
NMPDR	fig|243233.4.peg.2558.
PATRIC	22609122. VBIMetCap22254_2661.
TIGR	MCA2629.
Phylogenomic databases
HOGENOM	HBG758022.
OMA	RVIDHDA.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_2629-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_METCA

Accession

Primary (citable) accession number: Q604B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program