Reviewed, UniProtKB/Swiss-Prot Q60490 (EBP_CAVPO)

Names and origin

Protein names

Recommended name:
    3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    EC=5.3.3.5
Alternative name(s):
    Cholestenol Delta-isomerase
    Delta(8)-Delta(7) sterol isomerase
      Short name=D8-D7 sterol isomerase
    Emopamil-binding protein

Gene names

Name:

EBP

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers By similarity.
Catalytic activity	5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Highly expressed in liver, bowel, adrenal gland, testis, ovary, and uterus and less expressed in brain, cerebellum, skeletal muscle, and heart.
Miscellaneous	Binds to the phenylalkylamine calcium-ion antagonist emopamil, an anti-ischemic drug.
Sequence similarities	Belongs to the EBP family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholestenol delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 229	228	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase		PRO_0000174341
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	67 – 87	21	Potential
	Transmembrane	119 – 139	21	Potential
	Transmembrane	186 – 206	21	Potential
Experimental info
	Sequence conflict	27	1	L → Y AA sequence Ref.2
	Sequence conflict	31	1	Y → V AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q60490-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2D95E07F3248B2E6	FASTA	229	26,679
	10 20 30 40 50 60 MATTSTGPLH PYWPRHLRLD HFVPNDLSAW YIVTVLFTVF GALVVTMWLL SSRASVVPLG 70 80 90 100 110 120 TWRRLSVCWF AVCAFVHLVI EGWFVLYQKA ILGDQAFLSQ LWKEYAKGDS RYIIEDNFII 130 140 150 160 170 180 CMESITVVLW GPLSLWAVIA FLRQHPSRYV LQFVISLGQI YGDLLYFLTE YRDGFQHGEM 190 200 210 220 GHPIYFWFYF FFMNVLWLVI PGVLFFDSVK QFYGAQNALD TKVMKSKGK

References

[1]	"Phenylalkylamine Ca2+ antagonist binding protein. Molecular cloning, tissue distribution, and heterologous expression." Hanner M., Moebius F.F., Weber F., Grabner M., Striessnig J., Glossmann H. J. Biol. Chem. 270:7551-7557(1995) [PubMed: 7706302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Pirbright white. Tissue: Liver.
[2]	"Purification and amino-terminal sequencing of the high affinity phenylalkylamine Ca2+ antagonist binding protein from guinea pig liver endoplasmic reticulum." Moebius F.F., Hanner M., Knaus H.G., Weber F., Striessnig J., Glossmann H. J. Biol. Chem. 269:29314-29320(1994) [PubMed: 7961902] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-34. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z37985 mRNA. Translation: CAA86067.1.
PIR	A56122.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q60490.
Genome annotation databases
Ensembl	ENSCPOT00000014293; ENSCPOP00000012749; ENSCPOG00000014151; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	Q60490.
OMA	PTWHILA.
Enzyme and pathway databases
BRENDA	5.3.3.5. 44.
Family and domain databases
InterPro	IPR007905. EBP. [Graphical view]
PANTHER	PTHR14207. EBP. 1 hit.
Pfam	PF05241. EBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBP_CAVPO

Accession

Primary (citable) accession number: Q60490
Secondary accession number(s): Q9QV23

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)