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Q60487

- FGF2_CAVPO

UniProt

Q60487 - FGF2_CAVPO

Protein

Fibroblast growth factor 2

Gene

FGF2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (01 May 2000)
      Previous versions | rss
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    Functioni

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511HeparinBy similarity

    GO - Molecular functioni

    1. chemoattractant activity Source: Ensembl
    2. heparin binding Source: UniProtKB-KW
    3. ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl

    GO - Biological processi

    1. activation of MAPKK activity Source: Ensembl
    2. branching involved in ureteric bud morphogenesis Source: UniProtKB
    3. cell migration involved in sprouting angiogenesis Source: Ensembl
    4. chondroblast differentiation Source: Ensembl
    5. corticotropin hormone secreting cell differentiation Source: Ensembl
    6. fibroblast growth factor receptor signaling pathway Source: Ensembl
    7. glial cell differentiation Source: Ensembl
    8. hyaluronan catabolic process Source: Ensembl
    9. inositol phosphate biosynthetic process Source: Ensembl
    10. lung development Source: Ensembl
    11. mammary gland epithelial cell differentiation Source: Ensembl
    12. negative regulation of blood vessel endothelial cell migration Source: Ensembl
    13. negative regulation of cell death Source: UniProtKB
    14. negative regulation of cell proliferation Source: Ensembl
    15. negative regulation of fibroblast migration Source: Ensembl
    16. negative regulation of wound healing Source: Ensembl
    17. organ induction Source: Ensembl
    18. phosphatidylinositol biosynthetic process Source: Ensembl
    19. positive regulation of angiogenesis Source: Ensembl
    20. positive regulation of blood vessel endothelial cell migration Source: Ensembl
    21. positive regulation of cardiac muscle cell proliferation Source: Ensembl
    22. positive regulation of cell division Source: UniProtKB-KW
    23. positive regulation of cell fate specification Source: Ensembl
    24. positive regulation of cerebellar granule cell precursor proliferation Source: Ensembl
    25. positive regulation of endothelial cell proliferation Source: Ensembl
    26. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    27. positive regulation of osteoblast differentiation Source: Ensembl
    28. positive regulation of phosphatidylinositol 3-kinase activity Source: Ensembl
    29. positive regulation of phospholipase C activity Source: Ensembl
    30. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    31. regulation of cell cycle Source: Ensembl
    32. regulation of retinal cell programmed cell death Source: Ensembl
    33. release of sequestered calcium ion into cytosol Source: Ensembl
    34. response to axon injury Source: Ensembl
    35. stem cell development Source: Ensembl
    36. substantia nigra development Source: Ensembl
    37. thyroid-stimulating hormone-secreting cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein, Growth factor, Mitogen

    Keywords - Biological processi

    Angiogenesis, Differentiation

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fibroblast growth factor 2
    Short name:
    FGF-2
    Alternative name(s):
    Basic fibroblast growth factor
    Short name:
    bFGF
    Heparin-binding growth factor 2
    Short name:
    HBGF-2
    Prostatic growth factor
    Gene namesi
    Name:FGF2
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    Secreted By similarity. Nucleus By similarity
    Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. extracellular space Source: Ensembl
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 170›170Fibroblast growth factor 2PRO_0000008930Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41Omega-N-methylarginine; alternate1 Publication
    Modified residuei4 – 41Symmetric dimethylarginine; alternate1 Publication
    Modified residuei6 – 61Omega-N-methylarginine; alternate1 Publication
    Modified residuei6 – 61Symmetric dimethylarginine; alternate1 Publication
    Modified residuei8 – 81Omega-N-methylarginine; alternate1 Publication
    Modified residuei8 – 81Symmetric dimethylarginine; alternate1 Publication
    Modified residuei97 – 971Phosphotyrosine; by TECBy similarity

    Post-translational modificationi

    The N-terminus of isoform 2 is blocked.Curated
    Phosphorylation at Tyr-97 regulates FGF2 unconventional secretion.By similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000004847.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60487.
    SMRiQ60487. Positions 22-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni143 – 15917Heparin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG325757.
    HOVERGENiHBG107917.
    InParanoidiQ60487.

    Family and domain databases

    InterProiIPR008996. Cytokine_IL1-like.
    IPR028223. FGF2.
    IPR002209. Fibroblast_GF_fam.
    IPR028142. IL-1_fam/FGF_fam.
    [Graphical view]
    PANTHERiPTHR11486. PTHR11486. 1 hit.
    PTHR11486:SF68. PTHR11486:SF68. 1 hit.
    PfamiPF00167. FGF. 1 hit.
    [Graphical view]
    PRINTSiPR00263. HBGFFGF.
    PR00262. IL1HBGF.
    SMARTiSM00442. FGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF50353. SSF50353. 1 hit.
    PROSITEiPS00247. HBGF_FGF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Fragments.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: Q60487-1) [UniParc]FASTAAdd to Basket

    Also known as: 25 kDa

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    VGGRGRGRGT AAAARREPGG AMAAGSITTL PALPEGGDGG AFAPGHFKDP    50
    NGGFFLRIHP DGRVDGVREK TDPHIKLQLQ AEDRGVVSIK GVCANRYLAM 100
    KEDGRLLASK CVTDECFFFE RLESNNYNTY RSRKYSSWYV ALKRTGQYKL 150
    GSKTGPGQKA ILFLPMSAKS 170
    Length:170
    Mass (Da):18,354
    Last modified:May 1, 2000 - v2
    Checksum:iF36BDBC736E5FEBE
    GO
    Isoform 2 (identifier: Q60487-2) [UniParc]FASTAAdd to Basket

    Also known as: 18 kDa

    The sequence of this isoform differs from the canonical sequence as follows:
         1-21: Missing.

    Show »
    Length:149
    Mass (Da):16,392
    Checksum:i7A246D9C9C806B87
    GO

    Sequence cautioni

    The sequence AAA85394.1 differs from that shown. Reason: Frameshift at positions 77, 88, 93 and 149. The correction of the frameshifts allows to extend the similarity to the human sequence and is based on partial amino-acid sequencing.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-adjacent residuesi15 – 162Curated
    Non-adjacent residuesi50 – 512Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei‹1 – 21›21Missing in isoform 2. CuratedVSP_018726Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L75974 mRNA. Translation: AAA85394.1. Frameshift.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L75974 mRNA. Translation: AAA85394.1 . Frameshift.

    3D structure databases

    ProteinModelPortali Q60487.
    SMRi Q60487. Positions 22-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000004847.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG325757.
    HOVERGENi HBG107917.
    InParanoidi Q60487.

    Family and domain databases

    InterProi IPR008996. Cytokine_IL1-like.
    IPR028223. FGF2.
    IPR002209. Fibroblast_GF_fam.
    IPR028142. IL-1_fam/FGF_fam.
    [Graphical view ]
    PANTHERi PTHR11486. PTHR11486. 1 hit.
    PTHR11486:SF68. PTHR11486:SF68. 1 hit.
    Pfami PF00167. FGF. 1 hit.
    [Graphical view ]
    PRINTSi PR00263. HBGFFGF.
    PR00262. IL1HBGF.
    SMARTi SM00442. FGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50353. SSF50353. 1 hit.
    PROSITEi PS00247. HBGF_FGF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factor."
      Sommer A., Moscatelli D., Rifkin D.B.
      Biochem. Biophys. Res. Commun. 160:1267-1274(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, ALTERNATIVE INITIATION.
    2. Ricciardelli C.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-170.
      Tissue: Prostate.
    3. "Direct evidence for methylation of arginine residues in high molecular weight forms of basic fibroblast growth factor."
      Burgess W.H., Bizik J., Mehlman T., Quarto N., Rifkin D.B.
      Cell Regul. 2:87-93(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, METHYLATION AT ARG-4; ARG-6 AND ARG-8.
    4. "Mr 25,000 heparin-binding protein from guinea pig brain is a high molecular weight form of basic fibroblast growth factor."
      Moscatelli D., Joseph-Silverstein J., Manejias R., Rifkin D.B.
      Proc. Natl. Acad. Sci. U.S.A. 84:5778-5782(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Tissue: Brain.

    Entry informationi

    Entry nameiFGF2_CAVPO
    AccessioniPrimary (citable) accession number: Q60487
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3