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Q60487 (FGF2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fibroblast growth factor 2

Short name=FGF-2
Alternative name(s):
Basic fibroblast growth factor
Short name=bFGF
Heparin-binding growth factor 2
Short name=HBGF-2
Prostatic growth factor
Gene names
Name:FGF2
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length170 AA.
Sequence statusFragments.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.

Subunit structure

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.

Subcellular location

Secreted By similarity. Nucleus By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 By similarity.

Post-translational modification

The N-terminus of isoform 2 is blocked Probable.

Phosphorylation at Tyr-97 regulates FGF2 unconventional secretion By similarity.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Sequence caution

The sequence AAA85394.1 differs from that shown. Reason: Frameshift at positions 77, 88, 93 and 149. The correction of the frameshifts allows to extend the similarity to the human sequence and is based on partial amino-acid sequencing.

Ontologies

Keywords
   Biological processAngiogenesis
Differentiation
   Cellular componentNucleus
Secreted
   Coding sequence diversityAlternative initiation
   LigandHeparin-binding
   Molecular functionDevelopmental protein
Growth factor
Mitogen
   PTMMethylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPKK activity

Inferred from electronic annotation. Source: Ensembl

branching involved in ureteric bud morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

cell migration involved in sprouting angiogenesis

Inferred from electronic annotation. Source: Ensembl

chondroblast differentiation

Inferred from electronic annotation. Source: Ensembl

corticotropin hormone secreting cell differentiation

Inferred from electronic annotation. Source: Ensembl

fibroblast growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

glial cell differentiation

Inferred from electronic annotation. Source: Ensembl

hyaluronan catabolic process

Inferred from electronic annotation. Source: Ensembl

inositol phosphate biosynthetic process

Inferred from electronic annotation. Source: Ensembl

lung development

Inferred from electronic annotation. Source: Ensembl

mammary gland epithelial cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of blood vessel endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell death

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of fibroblast migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of wound healing

Inferred from electronic annotation. Source: Ensembl

organ induction

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of blood vessel endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cell fate specification

Inferred from electronic annotation. Source: Ensembl

positive regulation of cerebellar granule cell precursor proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphatidylinositol 3-kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of phospholipase C activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

regulation of cell cycle

Inferred from electronic annotation. Source: Ensembl

regulation of retinal cell programmed cell death

Inferred from electronic annotation. Source: Ensembl

release of sequestered calcium ion into cytosol

Inferred from electronic annotation. Source: Ensembl

response to axon injury

Inferred from electronic annotation. Source: Ensembl

stem cell development

Inferred from electronic annotation. Source: Ensembl

substantia nigra development

Inferred from electronic annotation. Source: Ensembl

thyroid-stimulating hormone-secreting cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchemoattractant activity

Inferred from electronic annotation. Source: Ensembl

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

ligand-dependent nuclear receptor transcription coactivator activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q60487-1)

Also known as: 25 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q60487-2)

Also known as: 18 kDa;

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 170›170Fibroblast growth factor 2
PRO_0000008930

Regions

Region143 – 15917Heparin-binding By similarity

Sites

Binding site511Heparin By similarity

Amino acid modifications

Modified residue41Omega-N-methylarginine; alternate Ref.3
Modified residue41Symmetric dimethylarginine; alternate Ref.3
Modified residue61Omega-N-methylarginine; alternate Ref.3
Modified residue61Symmetric dimethylarginine; alternate Ref.3
Modified residue81Omega-N-methylarginine; alternate Ref.3
Modified residue81Symmetric dimethylarginine; alternate Ref.3
Modified residue971Phosphotyrosine; by TEC By similarity

Natural variations

Alternative sequence‹1 – 21›21Missing in isoform 2.
VSP_018726

Experimental info

Non-adjacent residues15 – 162
Non-adjacent residues50 – 512
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (25 kDa) [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: F36BDBC736E5FEBE

FASTA17018,354
        10         20         30         40         50         60 
VGGRGRGRGT AAAARREPGG AMAAGSITTL PALPEGGDGG AFAPGHFKDP NGGFFLRIHP 

        70         80         90        100        110        120 
DGRVDGVREK TDPHIKLQLQ AEDRGVVSIK GVCANRYLAM KEDGRLLASK CVTDECFFFE 

       130        140        150        160        170 
RLESNNYNTY RSRKYSSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS 

« Hide

Isoform 2 (18 kDa) [UniParc].

Checksum: 7A246D9C9C806B87
Show »

FASTA14916,392

References

[1]"An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factor."
Sommer A., Moscatelli D., Rifkin D.B.
Biochem. Biophys. Res. Commun. 160:1267-1274(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, ALTERNATIVE INITIATION.
[2]Ricciardelli C.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-170.
Tissue: Prostate.
[3]"Direct evidence for methylation of arginine residues in high molecular weight forms of basic fibroblast growth factor."
Burgess W.H., Bizik J., Mehlman T., Quarto N., Rifkin D.B.
Cell Regul. 2:87-93(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, METHYLATION AT ARG-4; ARG-6 AND ARG-8.
[4]"Mr 25,000 heparin-binding protein from guinea pig brain is a high molecular weight form of basic fibroblast growth factor."
Moscatelli D., Joseph-Silverstein J., Manejias R., Rifkin D.B.
Proc. Natl. Acad. Sci. U.S.A. 84:5778-5782(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L75974 mRNA. Translation: AAA85394.1. Frameshift.

3D structure databases

ProteinModelPortalQ60487.
SMRQ60487. Positions 22-170.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000004847.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG325757.
HOVERGENHBG107917.
InParanoidQ60487.

Family and domain databases

InterProIPR008996. Cytokine_IL1-like.
IPR028223. FGF2.
IPR002209. Fibroblast_GF_fam.
IPR028142. IL-1_fam/FGF_fam.
[Graphical view]
PANTHERPTHR11486. PTHR11486. 1 hit.
PTHR11486:SF68. PTHR11486:SF68. 1 hit.
PfamPF00167. FGF. 1 hit.
[Graphical view]
PRINTSPR00263. HBGFFGF.
PR00262. IL1HBGF.
SMARTSM00442. FGF. 1 hit.
[Graphical view]
SUPFAMSSF50353. SSF50353. 1 hit.
PROSITEPS00247. HBGF_FGF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFGF2_CAVPO
AccessionPrimary (citable) accession number: Q60487
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families