Q60487 (FGF2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 2
Short name=FGF-2

Alternative name(s):
Basic fibroblast growth factor
Short name=bFGF
Heparin-binding growth factor 2
Short name=HBGF-2
Prostatic growth factor

Gene names

Name:

FGF2

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	170 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.
Subcellular location	Secreted By similarity. Nucleus By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 By similarity.
Post-translational modification	The N-terminus of isoform 2 is blocked Probable. Phosphorylation at Tyr-97 regulates FGF2 unconventional secretion By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.
Sequence caution	The sequence AAA85394.1 differs from that shown. Reason: Frameshift at positions 77, 88, 93 and 149. The correction of the frameshifts allows to extend the similarity to the human sequence and is based on partial amino-acid sequencing.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Nucleus Secreted
Coding sequence diversity	Alternative initiation
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPKK activity Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: Compara branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB cell migration involved in sprouting angiogenesis Inferred from electronic annotation. Source: Compara chondroblast differentiation Inferred from electronic annotation. Source: Compara corticotropin hormone secreting cell differentiation Inferred from electronic annotation. Source: Compara fibroblast growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara glial cell differentiation Inferred from electronic annotation. Source: Compara hyaluronan catabolic process Inferred from electronic annotation. Source: Compara inositol phosphate biosynthetic process Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara mammary gland epithelial cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of fibroblast migration Inferred from electronic annotation. Source: Compara negative regulation of wound healing Inferred from electronic annotation. Source: Compara organ induction Inferred from electronic annotation. Source: Compara phosphatidylinositol biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell fate specification Inferred from electronic annotation. Source: Compara positive regulation of cerebellar granule cell precursor proliferation Inferred from electronic annotation. Source: Compara positive regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of phosphatidylinositol 3-kinase activity Inferred from electronic annotation. Source: Compara positive regulation of phospholipase C activity Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of cell cycle Inferred from electronic annotation. Source: Compara regulation of retinal cell programmed cell death Inferred from electronic annotation. Source: Compara release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara response to axon injury Inferred from electronic annotation. Source: Compara stem cell development Inferred from electronic annotation. Source: Compara substantia nigra development Inferred from electronic annotation. Source: Compara thyroid-stimulating hormone-secreting cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chemoattractant activity Inferred from electronic annotation. Source: Compara heparin binding Inferred from electronic annotation. Source: UniProtKB-KW ligand-dependent nuclear receptor transcription coactivator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q60487-1)

Also known as: 25 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60487-2)

Also known as: 18 kDa;

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 170	›170	Fibroblast growth factor 2		PRO_0000008930
Regions
	Region	143 – 159	17	Heparin-binding By similarity
Sites
	Binding site	51	1	Heparin By similarity
Amino acid modifications
	Modified residue	4	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	4	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	6	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	6	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	8	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	8	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	97	1	Phosphotyrosine; by TEC By similarity
Natural variations
	Alternative sequence	‹1 – 21	›21	Missing in isoform 2.		VSP_018726
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	50 – 51	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (25 kDa) [UniParc]. Last modified May 1, 2000. Version 2. Checksum: F36BDBC736E5FEBE	FASTA	170	18,354
	10 20 30 40 50 60 VGGRGRGRGT AAAARREPGG AMAAGSITTL PALPEGGDGG AFAPGHFKDP NGGFFLRIHP 70 80 90 100 110 120 DGRVDGVREK TDPHIKLQLQ AEDRGVVSIK GVCANRYLAM KEDGRLLASK CVTDECFFFE 130 140 150 160 170 RLESNNYNTY RSRKYSSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
	Isoform 2 (18 kDa) [UniParc]. Checksum: 7A246D9C9C806B87	FASTA	149	16,392
	10 20 30 40 50 60 MAAGSITTLP ALPEGGDGGA FAPGHFKDPN GGFFLRIHPD GRVDGVREKT DPHIKLQLQA 70 80 90 100 110 120 EDRGVVSIKG VCANRYLAMK EDGRLLASKC VTDECFFFER LESNNYNTYR SRKYSSWYVA 130 140 LKRTGQYKLG SKTGPGQKAI LFLPMSAKS

References

[1]	"An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factor." Sommer A., Moscatelli D., Rifkin D.B. Biochem. Biophys. Res. Commun. 160:1267-1274(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, ALTERNATIVE INITIATION.
[2]	Ricciardelli C. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-170. Tissue: Prostate.
[3]	"Direct evidence for methylation of arginine residues in high molecular weight forms of basic fibroblast growth factor." Burgess W.H., Bizik J., Mehlman T., Quarto N., Rifkin D.B. Cell Regul. 2:87-93(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, METHYLATION AT ARG-4; ARG-6 AND ARG-8.
[4]	"Mr 25,000 heparin-binding protein from guinea pig brain is a high molecular weight form of basic fibroblast growth factor." Moscatelli D., Joseph-Silverstein J., Manejias R., Rifkin D.B. Proc. Natl. Acad. Sci. U.S.A. 84:5778-5782(1987) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L75974 mRNA. Translation: AAA85394.1. Frameshift.
3D structure databases
ProteinModelPortal	Q60487.
SMR	Q60487. Positions 22-170.
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000004847.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG325757.
HOVERGEN	HBG107917.
InParanoid	Q60487.
OrthoDB	EOG4K0QPR.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002209. GF_heparin-bd. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. PTHR11486. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00263. HBGFFGF. PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FGF2_CAVPO

Accession

Primary (citable) accession number: Q60487

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program