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Q60487

- FGF2_CAVPO

UniProt

Q60487 - FGF2_CAVPO

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Protein

Fibroblast growth factor 2

Gene

FGF2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511HeparinBy similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW

GO - Biological processi

  1. activation of MAPKK activity Source: Ensembl
  2. angiogenesis Source: UniProtKB-KW
  3. branching involved in ureteric bud morphogenesis Source: UniProtKB
  4. corticotropin hormone secreting cell differentiation Source: Ensembl
  5. fibroblast growth factor receptor signaling pathway Source: Ensembl
  6. glial cell differentiation Source: Ensembl
  7. lung development Source: Ensembl
  8. mammary gland epithelial cell differentiation Source: Ensembl
  9. negative regulation of cell death Source: UniProtKB
  10. negative regulation of cell proliferation Source: Ensembl
  11. organ induction Source: Ensembl
  12. positive regulation of angiogenesis Source: Ensembl
  13. positive regulation of canonical Wnt signaling pathway Source: Ensembl
  14. positive regulation of cell division Source: UniProtKB-KW
  15. positive regulation of cerebellar granule cell precursor proliferation Source: Ensembl
  16. positive regulation of endothelial cell migration Source: Ensembl
  17. positive regulation of endothelial cell proliferation Source: Ensembl
  18. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  19. positive regulation of osteoblast differentiation Source: Ensembl
  20. positive regulation of protein kinase B signaling Source: Ensembl
  21. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  22. regulation of cell cycle Source: Ensembl
  23. regulation of retinal cell programmed cell death Source: Ensembl
  24. response to axon injury Source: Ensembl
  25. stem cell development Source: Ensembl
  26. substantia nigra development Source: Ensembl
  27. thyroid-stimulating hormone-secreting cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fibroblast growth factor 2
Short name:
FGF-2
Alternative name(s):
Basic fibroblast growth factor
Short name:
bFGF
Heparin-binding growth factor 2
Short name:
HBGF-2
Prostatic growth factor
Gene namesi
Name:FGF2
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

Secreted By similarity. Nucleus By similarity
Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 By similarity.By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. extracellular space Source: Ensembl
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 170›170Fibroblast growth factor 2PRO_0000008930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Omega-N-methylarginine; alternate1 Publication
Modified residuei4 – 41Symmetric dimethylarginine; alternate1 Publication
Modified residuei6 – 61Omega-N-methylarginine; alternate1 Publication
Modified residuei6 – 61Symmetric dimethylarginine; alternate1 Publication
Modified residuei8 – 81Omega-N-methylarginine; alternate1 Publication
Modified residuei8 – 81Symmetric dimethylarginine; alternate1 Publication
Modified residuei97 – 971Phosphotyrosine; by TECBy similarity

Post-translational modificationi

The N-terminus of isoform 2 is blocked.Curated
Phosphorylation at Tyr-97 regulates FGF2 unconventional secretion.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000004847.

Structurei

3D structure databases

ProteinModelPortaliQ60487.
SMRiQ60487. Positions 22-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 15917Heparin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG325757.
HOVERGENiHBG107917.
InParanoidiQ60487.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR028223. FGF2.
IPR002209. Fibroblast_GF_fam.
IPR028142. IL-1_fam/FGF_fam.
[Graphical view]
PANTHERiPTHR11486. PTHR11486. 1 hit.
PTHR11486:SF68. PTHR11486:SF68. 1 hit.
PRINTSiPR00263. HBGFFGF.
PR00262. IL1HBGF.
SMARTiSM00442. FGF. 1 hit.
[Graphical view]
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00247. HBGF_FGF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Fragments.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: Q60487-1) [UniParc]FASTAAdd to Basket

Also known as: 25 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
VGGRGRGRGT AAAARREPGG AMAAGSITTL PALPEGGDGG AFAPGHFKDP
60 70 80 90 100
NGGFFLRIHP DGRVDGVREK TDPHIKLQLQ AEDRGVVSIK GVCANRYLAM
110 120 130 140 150
KEDGRLLASK CVTDECFFFE RLESNNYNTY RSRKYSSWYV ALKRTGQYKL
160 170
GSKTGPGQKA ILFLPMSAKS
Length:170
Mass (Da):18,354
Last modified:May 1, 2000 - v2
Checksum:iF36BDBC736E5FEBE
GO
Isoform 2 (identifier: Q60487-2) [UniParc]FASTAAdd to Basket

Also known as: 18 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Show »
Length:149
Mass (Da):16,392
Checksum:i7A246D9C9C806B87
GO

Sequence cautioni

The sequence AAA85394.1 differs from that shown. Reason: Frameshift at positions 77, 88, 93 and 149. The correction of the frameshifts allows to extend the similarity to the human sequence and is based on partial amino-acid sequencing.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi15 – 162Curated
Non-adjacent residuesi50 – 512Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei‹1 – 21›21Missing in isoform 2. CuratedVSP_018726Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L75974 mRNA. Translation: AAA85394.1. Frameshift.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L75974 mRNA. Translation: AAA85394.1 . Frameshift.

3D structure databases

ProteinModelPortali Q60487.
SMRi Q60487. Positions 22-170.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000004847.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG325757.
HOVERGENi HBG107917.
InParanoidi Q60487.

Family and domain databases

InterProi IPR008996. Cytokine_IL1-like.
IPR028223. FGF2.
IPR002209. Fibroblast_GF_fam.
IPR028142. IL-1_fam/FGF_fam.
[Graphical view ]
PANTHERi PTHR11486. PTHR11486. 1 hit.
PTHR11486:SF68. PTHR11486:SF68. 1 hit.
PRINTSi PR00263. HBGFFGF.
PR00262. IL1HBGF.
SMARTi SM00442. FGF. 1 hit.
[Graphical view ]
SUPFAMi SSF50353. SSF50353. 1 hit.
PROSITEi PS00247. HBGF_FGF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factor."
    Sommer A., Moscatelli D., Rifkin D.B.
    Biochem. Biophys. Res. Commun. 160:1267-1274(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, ALTERNATIVE INITIATION.
  2. Ricciardelli C.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-170.
    Tissue: Prostate.
  3. "Direct evidence for methylation of arginine residues in high molecular weight forms of basic fibroblast growth factor."
    Burgess W.H., Bizik J., Mehlman T., Quarto N., Rifkin D.B.
    Cell Regul. 2:87-93(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, METHYLATION AT ARG-4; ARG-6 AND ARG-8.
  4. "Mr 25,000 heparin-binding protein from guinea pig brain is a high molecular weight form of basic fibroblast growth factor."
    Moscatelli D., Joseph-Silverstein J., Manejias R., Rifkin D.B.
    Proc. Natl. Acad. Sci. U.S.A. 84:5778-5782(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Brain.

Entry informationi

Entry nameiFGF2_CAVPO
AccessioniPrimary (citable) accession number: Q60487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3