Q60487 (FGF2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparin-binding growth factor 2
Short name=HBGF-2

Alternative name(s):
Basic fibroblast growth factor
Short name=bFGF
Fibroblast growth factor 2
Short name=FGF-2
Prostatic growth factor

Gene names

Name:

FGF2

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	170 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 By similarity.
Subcellular location	Secreted By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane By similarity.
Post-translational modification	The N-terminus of isoform 2 is blocked Probable. Phosphorylation at Tyr-97 regulates FGF2 unconventional secretion By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.
Sequence caution	The sequence AAA85394.1 differs from that shown. Reason: Frameshift at positions 77, 88, 93 and 149. The correction of the frameshifts allows to extend the similarity to the human sequence and is based on partial amino-acid sequencing.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Secreted
Coding sequence diversity	Alternative initiation
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Methylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell death Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q60487-1)

Also known as: 25 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q60487-2)

Also known as: 18 kDa;

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 170	›170	Heparin-binding growth factor 2		PRO_0000008930
Regions
	Region	143 – 159	17	Heparin-binding By similarity
Sites
	Binding site	51	1	Heparin By similarity
Amino acid modifications
	Modified residue	4	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	4	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	6	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	6	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	8	1	Omega-N-methylarginine; alternate Ref.3
	Modified residue	8	1	Symmetric dimethylarginine; alternate Ref.3
	Modified residue	97	1	Phosphotyrosine; by TEC By similarity
Natural variations
	Alternative sequence	‹1 – 21	›21	Missing in isoform 2.		VSP_018726
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	50 – 51	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (25 kDa) [UniParc]. Last modified May 1, 2000. Version 2. Checksum: F36BDBC736E5FEBE	FASTA	170	18,354
	10 20 30 40 50 60 VGGRGRGRGT AAAARREPGG AMAAGSITTL PALPEGGDGG AFAPGHFKDP NGGFFLRIHP 70 80 90 100 110 120 DGRVDGVREK TDPHIKLQLQ AEDRGVVSIK GVCANRYLAM KEDGRLLASK CVTDECFFFE 130 140 150 160 170 RLESNNYNTY RSRKYSSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
	Isoform 2 (18 kDa) [UniParc]. Checksum: 7A246D9C9C806B87	FASTA	149	16,392
	10 20 30 40 50 60 MAAGSITTLP ALPEGGDGGA FAPGHFKDPN GGFFLRIHPD GRVDGVREKT DPHIKLQLQA 70 80 90 100 110 120 EDRGVVSIKG VCANRYLAMK EDGRLLASKC VTDECFFFER LESNNYNTYR SRKYSSWYVA 130 140 LKRTGQYKLG SKTGPGQKAI LFLPMSAKS

References

[1]	"An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factor." Sommer A., Moscatelli D., Rifkin D.B. Biochem. Biophys. Res. Commun. 160:1267-1274(1989) [PubMed: 2730645] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, ALTERNATIVE INITIATION.
[2]	Ricciardelli C. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-170. Tissue: Prostate.
[3]	"Direct evidence for methylation of arginine residues in high molecular weight forms of basic fibroblast growth factor." Burgess W.H., Bizik J., Mehlman T., Quarto N., Rifkin D.B. Cell Regul. 2:87-93(1991) [PubMed: 1713785] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, METHYLATION AT ARG-4; ARG-6 AND ARG-8.
[4]	"Mr 25,000 heparin-binding protein from guinea pig brain is a high molecular weight form of basic fibroblast growth factor." Moscatelli D., Joseph-Silverstein J., Manejias R., Rifkin D.B. Proc. Natl. Acad. Sci. U.S.A. 84:5778-5782(1987) [PubMed: 3475702] [Abstract] Cited for: CHARACTERIZATION. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L75974 mRNA. Translation: AAA85394.1. Frameshift.
3D structure databases
ProteinModelPortal	Q60487.
SMR	Q60487. Positions 22-170.
ModBase	Search...
Protein-protein interaction databases
STRING	Q60487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000005443; ENSCPOP00000004847; ENSCPOG00000005386.
Phylogenomic databases
eggNOG	roNOG16213.
GeneTree	ENSGT00600000084030.
HOVERGEN	HBG107917.
InParanoid	Q60487.
OrthoDB	EOG4K0QPR.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002209. GF_heparin-bd. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. IL1_HBGF. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00263. HBGFFGF. PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FGF2_CAVPO

Accession

Primary (citable) accession number: Q60487

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program