Q60478 (PDYN_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proenkephalin-B

Alternative name(s):
Beta-neoendorphin-dynorphin
Preprodynorphin

Cleaved into the following 9 chains:

1. Alpha-neoendorphin
2. Beta-neoendorphin
3. Big dynorphin
   Short name=Big Dyn
4. Dynorphin A(1-17)
   Short name=Dyn-A17
   Short name=Dynorphin A
5. Dynorphin A(1-13)
6. Dynorphin A(1-8)
7. Leu-enkephalin
8. Rimorphin
   Alternative name(s):
   Dynorphin B
   Short name=Dyn-B
   Dynorphin B(1-13)
9. Leumorphin
   Alternative name(s):
   Dynorphin B-29

Gene names

Name:

PDYN

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress By similarity. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin By similarity. Leumorphin has a typical opiod activity and may have anti-apoptotic effect By similarity.
Subcellular location	Secreted.
Post-translational modification	The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endorphin Neuropeptide Neurotransmitter Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 161	141			PRO_0000008169
	Peptide	164 – 173	10	Alpha-neoendorphin		PRO_0000306341
	Peptide	164 – 172	9	Beta-neoendorphin		PRO_0000008170
	Peptide	164 – 168	5	Leu-enkephalin By similarity		PRO_0000306342
	Propeptide	175 – 195	21			PRO_0000008171
	Peptide	198 – 229	32	Big dynorphin By similarity		PRO_0000306343
	Peptide	198 – 214	17	Dynorphin A(1-17)		PRO_0000008172
	Peptide	198 – 210	13	Dynorphin A(1-13) By similarity		PRO_0000306344
	Peptide	198 – 205	8	Dynorphin A(1-8)		PRO_0000306345
	Peptide	198 – 202	5	Leu-enkephalin By similarity		PRO_0000306346
	Peptide	217 – 245	29	Leumorphin		PRO_0000008173
	Peptide	217 – 229	13	Rimorphin By similarity		PRO_0000008174
	Peptide	217 – 221	5	Leu-enkephalin		PRO_0000008175

Sequences

	Sequence		Length	Mass (Da)
	Q60478 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 719B811A3683D19B	FASTA	245	27,509
	10 20 30 40 50 60 MVWPRLVLAA CLLAMPPAAA ECLSQCSLCA VRTQDGPKPI NPLICSLECQ ETLLPSEEWE 70 80 90 100 110 120 RCQGILSFFP SALRLRDQSG LDSRAALEEV YGELAKRARP FLQELEKSRF LPSTPAEDKF 130 140 150 160 170 180 RGLLGGLGEG LGSEAMGAPQ LNSGAVEAAA LDFDEDPKEQ VKRYGGFLRK YPKRSSEVAR 190 200 210 220 230 240 EGDRDGDNAG HEDLYKRYGG FLRRIRPKLK WDNQKRYGGF LRRQFKVVTR SQEDPNAYSE EFFDV

References

[1]	"Guinea pig preprodynorphin mRNA: primary structure and regional quantitation in the brain." Yuferov V.P., Laforge K.S., Spangler R., Maggos C.E., Kreek M.J. DNA Cell Biol. 15:1105-1112(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Adrenal gland and Brain.
[2]	"Measurement and chromatographic characterization of prodynorphin-derived peptides in the guinea-pig ileum." Steele P.A., Turner C.A., Murphy R. Neuropeptides 13:207-213(1989) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF DYNORPHIN A(1-8); DYNORPHIN A(1-17) AND ALPHA-NEOENDORPHIN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38912 mRNA. Translation: AAC18427.1.
PIR	A60410.
RefSeq	NP_001166468.1. NM_001172997.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000019436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000022266; ENSCPOP00000019436; ENSCPOG00000024291.
GeneID	100135596.
Organism-specific databases
CTD	5173.
Phylogenomic databases
eggNOG	NOG46156.
GeneTree	ENSGT00530000063761.
HOGENOM	HOG000013003.
HOVERGEN	HBG000063.
InParanoid	Q60478.
OMA	RRQFKVV.
OrthoDB	EOG4QFWFK.
Family and domain databases
InterPro	IPR006024. Opioid_neupept. IPR000750. Proenkphlin_B. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit. PTHR11438:SF4. PTHR11438:SF4. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01030. PENKBPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDYN_CAVPO

Accession

Primary (citable) accession number: Q60478

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program