Q60474 (ADA2A_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2A adrenergic receptor

Alternative name(s):
Alpha-2A adrenoreceptor
Short name=Alpha-2A adrenoceptor
Short name=Alpha-2AAR

Gene names

Name:

ADRA2A

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 By similarity.
Subunit structure	Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity by adrenergic receptor signaling pathway Inferred from electronic annotation. Source: Compara activation of protein kinase B activity Inferred from electronic annotation. Source: Compara adenylate cyclase-inhibiting adrenergic receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular response to hormone stimulus Inferred from electronic annotation. Source: Compara epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara fear response Inferred from electronic annotation. Source: Compara glucose homeostasis Inferred from electronic annotation. Source: Compara negative regulation of insulin secretion involved in cellular response to glucose stimulus Inferred from electronic annotation. Source: Compara negative regulation of lipid catabolic process Inferred from electronic annotation. Source: Compara phospholipase C-activating adrenergic receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of cytokine production Inferred from electronic annotation. Source: Compara positive regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara positive regulation of wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from electronic annotation. Source: Compara receptor complex Inferred from electronic annotation. Source: Compara
Molecular_function	alpha2-adrenergic receptor activity Inferred from electronic annotation. Source: Compara epinephrine binding Inferred from electronic annotation. Source: Compara norepinephrine binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Alpha-2A adrenergic receptor		PRO_0000069079
Regions
	Topological domain	1 – 33	33	Extracellular By similarity
	Transmembrane	34 – 59	26	Helical; Name=1; By similarity
	Topological domain	60 – 70	11	Cytoplasmic By similarity
	Transmembrane	71 – 96	26	Helical; Name=2; By similarity
	Topological domain	97 – 106	10	Extracellular By similarity
	Transmembrane	107 – 129	23	Helical; Name=3; By similarity
	Topological domain	130 – 149	20	Cytoplasmic By similarity
	Transmembrane	150 – 173	24	Helical; Name=4; By similarity
	Topological domain	174 – 192	19	Extracellular By similarity
	Transmembrane	193 – 217	25	Helical; Name=5; By similarity
	Topological domain	218 – 374	157	Cytoplasmic By similarity
	Transmembrane	375 – 399	25	Helical; Name=6; By similarity
	Topological domain	400 – 409	10	Extracellular By similarity
	Transmembrane	410 – 430	21	Helical; Name=7; By similarity
	Topological domain	431 – 450	20	Cytoplasmic By similarity
Sites
	Site	113	1	Implicated in ligand binding By similarity
	Site	199	1	Implicated in agonist binding and receptor activation By similarity
	Site	203	1	Implicated in agonist binding and receptor activation By similarity
Amino acid modifications
	Lipidation	442	1	S-palmitoyl cysteine By similarity
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	14	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60474 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E6BBEE9A7FFB8DD1	FASTA	450	48,911
	10 20 30 40 50 60 MGSLQPDSGN ASWNGTEGPG GGTRATPYSL QVTVTLVCLV GLLILLTVFG NVLVIIAVFT 70 80 90 100 110 120 SRALKAPQNL FLVSLASADI LVATLVIPFS LANEVMGYWY FGKAWCEIYL ALDVLFCTSS 130 140 150 160 170 180 IVHLCAISLD RYWSITQAIE YNLKRTPRRI KAIIVTVWVI SAVISFPPLI SFEKAGGGGQ 190 200 210 220 230 240 QPAEPRCEIN DQKWYVISSS IGSFFAPCLI MILVYVRIYQ IAKRRTRVPP SRRGPDAHAA 250 260 270 280 290 300 APPGGAERRP NGLGLERGVG PGGAEAEPLP TQVNGAPGEP APAGPRDAEA LDLEESSSSE 310 320 330 340 350 360 HAERPPGARR PERGLRAKSK ARASQVKPGD SLPRRAPGAA GSGTSGSGPG EERGGGAKAS 370 380 390 400 410 420 RWRGRQNREK RFTFVLAVVI GVFVVCWFPF FFTYTLTAVG CSVPRTLFKF FFWFGYCNSS 430 440 450 LNPVIYTIFN HDFRRAFKKI LCRGDRKRIV

References

[1]

"Heterologous expression of the cloned guinea pig alpha 2A, alpha 2B, and alpha 2C adrenoceptor subtypes. Radioligand binding and functional coupling to a cAMP-responsive reporter gene." Svensson S.P., Bailey T.J., Porter A.C., Richman J.G., Regan J.W. Biochem. Pharmacol. 51:291-300(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hartley.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25722 Genomic DNA. Translation: AAA67074.1.
3D structure databases
ProteinModelPortal	Q60474.
SMR	Q60474. Positions 118-149.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG249628.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	Q60474.
OrthoDB	EOG4R502W.
Family and domain databases
InterPro	IPR001946. Adren_rcpt_A2A. IPR002233. Adrnrgc_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00558. ADRENRGCA2AR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q60474.

Entry information

Entry name

ADA2A_CAVPO

Accession

Primary (citable) accession number: Q60474

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program