Reviewed, UniProtKB/Swiss-Prot Q60457 (SOAT1_CRIGR)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 1
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 1
    Acyl coenzyme A:cholesterol acyltransferase 1
      Short name=ACAT-1

Gene names

Name:	SOAT1
Synonyms:	ACAT, ACAT1

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Sterol O-acyltransferase 1		PRO_0000207639
Regions
	Transmembrane	138 – 158	21	Potential
	Transmembrane	317 – 337	21	Potential
	Transmembrane	358 – 378	21	Potential
	Transmembrane	466 – 486	21	Potential
	Transmembrane	494 – 514	21	Potential
Sites
	Active site	456	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60457-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4ED6C403AAC7E65B	FASTA	546	64,111
	10 20 30 40 50 60 MVGEEKMSLR NRLSKSGENP EQDEAQRSVS DTQSNGRITM KQLIAKKRQL AAEAEELKPL 70 80 90 100 110 120 FLKEVGCHFD DFVTNLIEKS ASLDNGGCAL TTFSILEEMK NNHRAKDLRA PPEKGKIFIS 130 140 150 160 170 180 RRSLLDELFE VDHIRTIYHM FIGLLILFIL STLVVDYIDE GRLVLEFNLL GYAFGKLPTV 190 200 210 220 230 240 IWTWWAMFLS TLSIPYFLFQ RWAHGYSKTS HPLIYSLSHG FFFLVFQLGI LGFVPTYVVL 250 260 270 280 290 300 AYTLPPASRF IVILEQIRMV MKAHSFVREN VPRVLNAAKE KSSTVPVPTV NQYLYFLFAP 310 320 330 340 350 360 TLIYRDSYPR TPTVRWGYVA VQFLQVFGCL FYVYYIFERL CAPLFRNIKQ EPFSARVLVL 370 380 390 400 410 420 CVFNSILPGV LMLFLTFFAF LHCWLNAFAE MLRFGDRMFY KDWWNSTSYS NYYRTWNVVV 430 440 450 460 470 480 HDWLYYYAYK DLLWFFSKRF KSAAMLAVFA LSAVVHEYAL AVCLSYFYPV LFVLFMFFGM 490 500 510 520 530 540 AFNFIVNDSR KRPIWNIMVW ASLFLGHGVI LCFYSQEWYA RQHCPLKNPT FLDYVRPRSW TCQYVF

References

[1]

"Complementation of mutation in acyl-CoA:cholesterol acyltransferase (ACAT) fails to restore sterol regulation in ACAT-defective sterol-resistant hamster cells." Cao G., Goldstein J.L., Brown M.S. J. Biol. Chem. 271:14642-14648(1996) [PubMed: 8662991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47320 mRNA. Translation: AAC52670.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q60457.
Enzyme and pathway databases
BRENDA	2.3.1.26. 18.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SOAT1_CRIGR

Accession

Primary (citable) accession number: Q60457

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)