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Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei197 – 1971FMNUniRule annotation
Binding sitei287 – 2871NADPUniRule annotation
Binding sitei413 – 4131FADUniRule annotation
Binding sitei467 – 4671FADUniRule annotation
Binding sitei524 – 5241NADPUniRule annotation
Binding sitei628 – 6281NADPUniRule annotation
Binding sitei666 – 6661FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 806FMNUniRule annotation
Nucleotide bindingi127 – 1304FMNUniRule annotation
Nucleotide bindingi162 – 17110FMNUniRule annotation
Nucleotide bindingi443 – 4464FADUniRule annotation
Nucleotide bindingi461 – 4633FADUniRule annotation
Nucleotide bindingi477 – 4804FADUniRule annotation
Nucleotide bindingi585 – 5862NADPUniRule annotation
Nucleotide bindingi591 – 5955NADPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Ligandi

FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PorUniRule annotation
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)Imported
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111LumenalUniRule annotationAdd
BLAST
Transmembranei12 – 3221HelicalUniRule annotationAdd
BLAST
Topological domaini33 – 667635CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulumUniRule annotation, Membrane

Structurei

3D structure databases

ProteinModelPortaliQ60451.
SMRiQ60451. Positions 53-667.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 213145Flavodoxin-likeInterPro annotationAdd
BLAST
Domaini268 – 510243FAD-binding FR-typeInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

HOVERGENiHBG000432.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAVAEEVS LFSTTDVVLF SLIVGVLTYW FIFRKKKEEV PEFSKIQTAT
60 70 80 90 100
PSVKESSFVE KMKKTGRNII VFYGSQTGTA EEFANRLSKD AHRYGMRGMS
110 120 130 140 150
ADPEEYDLAD LSSLPEIDKS LVVFCMATYG EGDPTDNAQD FYDWLQETDV
160 170 180 190 200
DLTGVKFAVF GLGNKTYEHF NAMGKYVDQR LEQLGAQRIF ELGLGDDDGN
210 220 230 240 250
LEEDFITWRE QFWPAVCEFF GVEATGEESS IRQYELLVHE DIDAAKVYTG
260 270 280 290 300
EMGRLKSYEN QKPPFDAKNP FLAAVTTNRK LNQGTERHLM HLELDISDSK
310 320 330 340 350
IRYESGDHVA VYPANDSTLV NQIGEILGAD LDVVMSLNNL DEESNKKHPF
360 370 380 390 400
PCPTTYRTAL TYYLDITNPP RTNVLYELAQ YASEPSEQEQ LHKMASSSGE
410 420 430 440 450
GKELYLSWVV EARRHILAIL QDYPSLRPPI DHLCELLPRL QARYYSIASS
460 470 480 490 500
SKVHPNSVHI CAVAVEYEAK SGRVNKGVAT SWLRAKEPAG ENGRRALVPM
510 520 530 540 550
FVRKSQFRLP FKSVTPVIMV GPGTGIAPFM GFIQERAWLR EQGKEVGETL
560 570 580 590 600
LYYGCRRSDE DYLYREELAR FHKDGALTQL NVAFSREQAH KVYVQHLLKR
610 620 630 640 650
DREHLWKLIH EGGAHIYVCG DARNMAKDVQ NTFYDIVAEF GPMEHAQAVD
660
YVKKLMTKGR YSLDVWS
Length:667
Mass (Da):75,851
Last modified:November 1, 1996 - v1
Checksum:i2023F43DF4C1EF07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83230 mRNA. Translation: BAA11856.1.
RefSeqiNP_001231204.1. NM_001244275.1.

Genome annotation databases

GeneIDi100689241.
KEGGicge:100689241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83230 mRNA. Translation: BAA11856.1.
RefSeqiNP_001231204.1. NM_001244275.1.

3D structure databases

ProteinModelPortaliQ60451.
SMRiQ60451. Positions 53-667.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689241.
KEGGicge:100689241.

Organism-specific databases

CTDi5447.

Phylogenomic databases

HOVERGENiHBG000432.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ60451_CRIGR
AccessioniPrimary (citable) accession number: Q60451
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.