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Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei197FMNUniRule annotation1
Binding sitei287NADPUniRule annotation1
Binding sitei413FADUniRule annotation1
Binding sitei467FADUniRule annotation1
Binding sitei524NADPUniRule annotation1
Binding sitei628NADPUniRule annotation1
Binding sitei666FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 80FMNUniRule annotation6
Nucleotide bindingi127 – 130FMNUniRule annotation4
Nucleotide bindingi162 – 171FMNUniRule annotation10
Nucleotide bindingi443 – 446FADUniRule annotation4
Nucleotide bindingi461 – 463FADUniRule annotation3
Nucleotide bindingi477 – 480FADUniRule annotation4
Nucleotide bindingi585 – 586NADPUniRule annotation2
Nucleotide bindingi591 – 595NADPUniRule annotation5

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Ligandi

FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PorUniRule annotation
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)Imported
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11LumenalUniRule annotationAdd BLAST11
Transmembranei12 – 32HelicalUniRule annotationAdd BLAST21
Topological domaini33 – 667CytoplasmicUniRule annotationAdd BLAST635

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulumUniRule annotation, Membrane

Structurei

3D structure databases

ProteinModelPortaliQ60451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 213Flavodoxin-likeInterPro annotationAdd BLAST145
Domaini268 – 510FAD-binding FR-typeInterPro annotationAdd BLAST243

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

HOVERGENiHBG000432.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAVAEEVS LFSTTDVVLF SLIVGVLTYW FIFRKKKEEV PEFSKIQTAT
60 70 80 90 100
PSVKESSFVE KMKKTGRNII VFYGSQTGTA EEFANRLSKD AHRYGMRGMS
110 120 130 140 150
ADPEEYDLAD LSSLPEIDKS LVVFCMATYG EGDPTDNAQD FYDWLQETDV
160 170 180 190 200
DLTGVKFAVF GLGNKTYEHF NAMGKYVDQR LEQLGAQRIF ELGLGDDDGN
210 220 230 240 250
LEEDFITWRE QFWPAVCEFF GVEATGEESS IRQYELLVHE DIDAAKVYTG
260 270 280 290 300
EMGRLKSYEN QKPPFDAKNP FLAAVTTNRK LNQGTERHLM HLELDISDSK
310 320 330 340 350
IRYESGDHVA VYPANDSTLV NQIGEILGAD LDVVMSLNNL DEESNKKHPF
360 370 380 390 400
PCPTTYRTAL TYYLDITNPP RTNVLYELAQ YASEPSEQEQ LHKMASSSGE
410 420 430 440 450
GKELYLSWVV EARRHILAIL QDYPSLRPPI DHLCELLPRL QARYYSIASS
460 470 480 490 500
SKVHPNSVHI CAVAVEYEAK SGRVNKGVAT SWLRAKEPAG ENGRRALVPM
510 520 530 540 550
FVRKSQFRLP FKSVTPVIMV GPGTGIAPFM GFIQERAWLR EQGKEVGETL
560 570 580 590 600
LYYGCRRSDE DYLYREELAR FHKDGALTQL NVAFSREQAH KVYVQHLLKR
610 620 630 640 650
DREHLWKLIH EGGAHIYVCG DARNMAKDVQ NTFYDIVAEF GPMEHAQAVD
660
YVKKLMTKGR YSLDVWS
Length:667
Mass (Da):75,851
Last modified:November 1, 1996 - v1
Checksum:i2023F43DF4C1EF07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83230 mRNA. Translation: BAA11856.1.
RefSeqiNP_001231204.1. NM_001244275.1.

Genome annotation databases

GeneIDi100689241.
KEGGicge:100689241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83230 mRNA. Translation: BAA11856.1.
RefSeqiNP_001231204.1. NM_001244275.1.

3D structure databases

ProteinModelPortaliQ60451.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689241.
KEGGicge:100689241.

Organism-specific databases

CTDi5447.

Phylogenomic databases

HOVERGENiHBG000432.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ60451_CRIGR
AccessioniPrimary (citable) accession number: Q60451
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.