Q60431 (CASP3_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase-3
Short name=CASP-3
EC=3.4.22.56

Alternative name(s):
Apopain
Cysteine protease CPP32
Short name=CPP-32
Protein Yama
SREBP cleavage activity 1
Short name=SCA-1

Cleaved into the following 2 chains:

1. Caspase-3 subunit p17
2. Caspase-3 subunit p12

Gene names

Name:	CASP3
Synonyms:	CPP32

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage By similarity.
Catalytic activity	Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit By similarity. Interacts with BIRC6/bruce By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa By similarity. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol By similarity.
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein S-nitrosylation Zymogen
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 9	9	By similarity		PRO_0000004561
	Propeptide	10 – 28	19	By similarity		PRO_0000004562
	Chain	29 – 175	147	Caspase-3 subunit p17		PRO_0000004563
	Chain	176 – 277	102	Caspase-3 subunit p12		PRO_0000004564
Sites
	Active site	121	1	By similarity
	Active site	163	1	By similarity
Amino acid modifications
	Modified residue	26	1	Phosphoserine By similarity
	Modified residue	163	1	S-nitrosocysteine; in inhibited form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60431 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0BF3A4590A2828A3	FASTA	277	31,612
	10 20 30 40 50 60 MENNETSVDS KSIKNFEVKT IHGSKSMDSG IYLDSSYKMD YPEMGVCIII NNKNFHKSTG 70 80 90 100 110 120 MTPRSGTDVD AAKLRETFMA LKYEVRNKND LTREEIVELM KNASKEDHSK RSSFVCVILS 130 140 150 160 170 180 HGDEGVIFGT DGPIDLKKLT SYFRGDYCRS LIGKPKLFII QACRGTELDC GIETDSGTED 190 200 210 220 230 240 DMTCQKIPVE ADFLYAYSTA PGYYSWRNPK DGSWFIQSLC SMLKLYAHKL EFMHILTRVN 250 260 270 RKVATEFESF SLDSTFHAKK QIPCIVSMLT KELYFYH

References

[1]	"Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis." Wang X., Zelenski N.G., Yang J., Sakai J., Brown M.S., Goldstein J.L. EMBO J. 15:1012-1020(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27463 mRNA. Translation: AAB01511.1.
PIR	S64710.
3D structure databases
ProteinModelPortal	Q60431.
SMR	Q60431. Positions 29-277.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-987N.
Protein family/group databases
MEROPS	C14.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050802.
Family and domain databases
InterPro	IPR015470. Caspase_3. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454. PTHR10454. 1 hit. PTHR10454:SF30. PTHR10454:SF30. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP3_MESAU

Accession

Primary (citable) accession number: Q60431

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program