Q603L4 (HEM6_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	MCA2791

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_0000109903
Regions
	Region	50 – 59	10	Important for dimerization By similarity
	Region	110 – 112	3	Substrate binding By similarity
	Region	242 – 277	36	Important for dimerization By similarity
	Region	260 – 265	6	Substrate binding By similarity
Sites
	Active site	108	1	Proton donor By similarity
	Binding site	94	1	Substrate By similarity
	Site	177	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q603L4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: FEF7B80BFA978D3F	FASTA	304	35,095
	10 20 30 40 50 60 MAGPDIEAVK SYLLDLQESI CNALETEEPR ARFVEDRWEH AAGGGGRTRV LSGGETFEQG 70 80 90 100 110 120 GVNFSHVRGA SLPASATAHR PELAGRGFQA TGVSLVIHPL NPYVPTSHAN VRFFLAEKEG 130 140 150 160 170 180 ETPIWWFGGG FDLTPFYPFE EDVIHWHRTA RNACLPFGED VYPRFKRWCD EYFFLNHRNE 190 200 210 220 230 240 TRGVGGLFFD DLNEWGFERS FAFLRSVGDH YLKAYLPIVQ RRKAIPYGER EREFQLYRRG 250 260 270 280 290 300 RYVEFNLVYD RGTLFGLQSG GRTESILMSL PPVAHWRYDW RPERGSAEEN LYLNYLKPKE WLES

References

[1]

"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017282 Genomic DNA. Translation: AAU91097.1.
RefSeq	YP_115186.1. NC_002977.6.
3D structure databases
ProteinModelPortal	Q603L4.
SMR	Q603L4. Positions 6-302.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3103617.
GenomeReviews	Gene locus MCA2791 in contig AE017282_GR.
KEGG	mca:MCA2791.
NMPDR	fig|243233.4.peg.2711.
PATRIC	22609458. VBIMetCap22254_2824.
TIGR	MCA2791.
Phylogenomic databases
HOGENOM	HBG631180.
OMA	VKAYLLD.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	MCAP243233:MCA_2791-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_METCA

Accession

Primary (citable) accession number: Q603L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program