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Q603G7 (Q603G7_METCA) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL AAU91143.1
EC=5.99.1.2 EMBL AAU91143.1
Gene names
Name:topA EMBL AAU91143.1
Ordered Locus Names:MCA2839
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP] EMBL AAU91143.1
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length768 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q603G7 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 28688534C6ADBE75

FASTA76886,941
        10         20         30         40         50         60 
MAQHLVIVES PAKARTIEKY LGKSFQVYAS YGHVRDLIPK EGAVDPDHDF SMKYAIIEKN 

        70         80         90        100        110        120 
KKHVQAISKA MEKADALYLA TDPDREGEAI SWHLYELLKE QQVLDSKPVH RVVFHEITKR 

       130        140        150        160        170        180 
AITEAVENPK TLSTDLIHAQ QARRALDYLV GFKLSPLLWK KIRRGLSAGR VQSPALRMIV 

       190        200        210        220        230        240 
ERELEIERFE VQEYWTIEAA IQTEDQALSA RLTHLAGEKL EQFSIVNEAQ AQATRQSLLD 

       250        260        270        280        290        300 
QARGKLQVVR VEERERKRNP AAPFTTSTLQ QEAARKLGFT TRRTMTVAQQ LYEGIDLGGE 

       310        320        330        340        350        360 
AVGLITYMRT DSVNLAQEAV QELRALIESR YGKDNLPAQP RLYKTKSKNA QEAHEAIRPT 

       370        380        390        400        410        420 
SAFRTPESVK AHLTPDQFKL YSLIWKRSVA CQMVHATLNT VTVDFACGSA DNLFRATGST 

       430        440        450        460        470        480 
VIHPGFMSVY REGRDDMPEE SDEIYLPKLV EGQEVDLKDV TPSQHFTEPP PRYTEASLVK 

       490        500        510        520        530        540 
ALEEYGIGRP STYATIISTL QQRHYVELEN KRFRPTDLGR VVNKFLTEHF NRYVDYNFTA 

       550        560        570        580        590        600 
NLEDDLDAVS RGEKDWIPLM REFWGPFHAL IGEKDESLKR ADVTHEAIDE KCPECGSPLS 

       610        620        630        640        650        660 
IRLGRNGRFV GCTNYPQCKY TRNLAGKESE QTEPEVVEGR QCPKCHSPLV IKTGRYGRFI 

       670        680        690        700        710        720 
GCSGYPACRH IEPLEKPTDT GVPCPECGQG TLTKRKSRFG KLFYSCSTYP KCSYAVWNPP 

       730        740        750        760 
IAEACPACQW PVLTLKTTKR RGTEKVCPRK ECGYAAPYEG EPLTDFAA

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017282 Genomic DNA. Translation: AAU91143.1.
RefSeqYP_115233.1. NC_002977.6.

3D structure databases

ProteinModelPortalQ603G7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3104042.
GenomeReviewsGene locus MCA2839 in contig AE017282_GR.
KEGGmca:MCA2839.
NMPDRfig|243233.4.peg.2758.
PATRIC22609552. VBIMetCap22254_2871.
TIGRMCA2839.

Phylogenomic databases

HOGENOMHBG512935.
OMATIPKERE.
ProtClustDBPRK06599.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ603G7_METCA
AccessionPrimary (citable) accession number: Q603G7
Entry history
Integrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info