Q60390 (MCRY_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase II subunit beta
Short name=MCR II beta
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta

Gene names

Name:	mrtB
Ordered Locus Names:	MJ0081

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide By similarity.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Methyl-coenzyme M reductase II subunit beta		PRO_0000147466

Sequences

	Sequence		Length	Mass (Da)
	Q60390 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: B9CAE1066BE977BC	FASTA	447	47,770
	10 20 30 40 50 60 MLHYEDRIDL YDERGKLLEE NVPLEAISPL KNPTIEKIVN DIKRSVAINL AGIENALKTG 70 80 90 100 110 120 AVGGKACFCP GRELDLPIVE NAEIIAEKIK RMVQIEEDDD TVVKLINGGK QLLLQLPSKR 130 140 150 160 170 180 LRVAADYTVS ALIGGGATVQ AIVDAFDVDM FDAPVVKTAV MGRYPQTVDF HGANIATLLG 190 200 210 220 230 240 PPVLLEGLGY GLRNIMANHI VAVTRKKTLN AVALASILEQ TAMFETGDAL GAFERLHLLG 250 260 270 280 290 300 LAFQGLNANN LTYELVKENG KDGTVGTVVA SVVERALEDG VIRPLKTMPS GFTVYEPVDW 310 320 330 340 350 360 ALWNAYAASG LVAAVIVNVG AARAAQGVAS TVLYYNDILE YETGLPSVDF GRAEGTAVGF 370 380 390 400 410 420 SFFSHSIYGG GGPGTFHGNH VVTRHSKGFA IPCAAAAMCL DAGTQMFSVE RTSALVGTVY 430 440 SAIDHLREPL KYVAEGAVEV KEKEKVI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98061.1.
RefSeq	NP_247045.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60390.
SMR	Q60390. Positions 4-443.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1450920.
GenomeReviews	Gene locus MJ0081 in contig L77117_GR.
KEGG	mja:MJ_0081.
NMPDR	fig|243232.1.peg.82.
TIGR	MJ0081.
Phylogenomic databases
HOGENOM	HBG538593.
OMA	WALWNAY.
ProtClustDB	CLSK876059.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0081-MONOMER.
Family and domain databases
InterPro	IPR022681. MCR_a/b_chain_a-bundle. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR015823. Me_CoM_Rdtase_asu_N_sub2. IPR003179. Me_CoM_Rdtase_bsu. IPR022679. Me_CoM_Rdtase_bsu_C. IPR022680. Me_CoM_Rdtase_bsu_N. IPR009024. Me_CoM_Rdtase_Fd-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 2 hits. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
KO	K00401.
Pfam	PF02241. MCR_beta. 1 hit. PF02783. MCR_beta_N. 1 hit. [Graphical view]
PIRSF	PIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAM	SSF48081. MCR_alpha_beta_C. 1 hit. SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03257. Met_CoM_red_bet. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRY_METJA

Accession

Primary (citable) accession number: Q60390

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program