Reviewed, UniProtKB/Swiss-Prot Q60382 (ARGB_METJA)

Names and origin

Protein names

Recommended name:
    Probable acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	MJ0069

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Probable acetylglutamate kinase HAMAP MF_00082		PRO_0000112694
Regions
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Binding site	90	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Site	33	1	Transition state stabilizer By similarity
	Site	257	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60382-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B54C58AAFA43F7DA	FASTA	300	32,725
	10 20 30 40 50 60 MGMDMIEMIE KAEILMEALP FIQKFYGKIF VIKYGGHAMI DEKAKNWTAQ DVVLLKYVGI 70 80 90 100 110 120 NPVVVHGGGP EINKAMEKMG KKPEFVHGLR VTDEETLDIV EMVLAGKING DIVSKLSKFG 130 140 150 160 170 180 GKAVGLSGKS GRIILAKKKL KKIKTEKGEE IEVDLGRVGE TVEVNTELLE ILINNGYIPV 190 200 210 220 230 240 VSPIGLDEKG EAYNLNADTV AGDIAGALKA EKLILITDVD GIMDDINNPE TLHRKLTASE 250 260 270 280 290 300 LKEMIEDGRI KGGMIPKAES ALYALEHGVK SVHIINGKIP HALLLEIFTE EGIGTMITRD

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98048.1.
PIR	E64308.
RefSeq	NP_247033.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450908.
GenomeReviews	Gene locus MJ0069 in contig L77117_GR.
KEGG	mja:MJ0069.
NMPDR	fig|243232.1.peg.70.
TIGR	MJ0069.
Phylogenomic databases
HOGENOM	Q60382.
OMA	MDIVEMV
Enzyme and pathway databases
BRENDA	2.7.2.8. 256362.
Family and domain databases
HAMAP	MF_00082. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_METJA

Accession

Primary (citable) accession number: Q60382

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)