Q60367 (MTIP_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable S-methyl-5'-thioinosine phosphorylase
EC=2.4.2.44

Alternative name(s):
5'-methylthioinosine phosphorylase
Short name=MTI phosphorylase
Short name=MTIP

Gene names

Ordered Locus Names:

MJ0060

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine By similarity. HAMAP MF_01963
Catalytic activity	S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate. HAMAP MF_01963
Pathway	Purine metabolism; purine nucleoside salvage. HAMAP MF_01963
Subunit structure	Homotrimer By similarity. HAMAP MF_01963
Miscellaneous	Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity. HAMAP MF_01963
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Ontologies

Keywords
Biological process	Purine salvage
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Probable S-methyl-5'-thioinosine phosphorylase HAMAP MF_01963		PRO_0000184559
Regions
	Region	44 – 45	2	Phosphate binding By similarity
	Region	197 – 199	3	Substrate binding By similarity
Sites
	Binding site	8	1	Phosphate By similarity
	Binding site	173	1	Substrate; via amide nitrogen By similarity
	Binding site	174	1	Phosphate By similarity
	Site	156	1	Important for substrate specificity By similarity
	Site	209	1	Important for substrate specificity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60367 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DFD7C01615774A79	FASTA	252	28,664
	10 20 30 40 50 60 MIGIIGGTGI AEILKGDKEE IINTKYGKAR VIIDKENEVV LLFRHGVRHN IPPHKINYRA 70 80 90 100 110 120 NIYALKKLGV ERILAINSVG SLKEDLKPGM FFVPNDFIEF TKKREETFYD EGKVVHIDMT 130 140 150 160 170 180 DPYCPELRNI LKSILDKNNF SYGEGVYVCT EGPRFETKKE IAIYKNWGDV VGMTGYPEVV 190 200 210 220 230 240 LARELEMCYV SLCNITNYAC GISKNILTVD EVLEKIKEME NKILKVVEDF INYGFGERKC 250 ICKDALKHAV IG

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98042.1.
PIR	D64307.
RefSeq	NP_247024.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1450899.
GenomeReviews	Gene locus MJ0060 in contig L77117_GR.
KEGG	mja:MJ_0060.
NMPDR	fig|243232.1.peg.61.
TIGR	MJ0060.
Phylogenomic databases
HOGENOM	HBG286690.
OMA	CFCKSAL.
ProtClustDB	CLSK876055.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0060-MONOMER.
Family and domain databases
HAMAP	MF_01963. MTAP. [Tree]
InterPro	IPR010044. MeThioAdo_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. IPR001369. Purine_phosphorylase-2. IPR018099. Purine_phosphorylase-2_CS. [Graphical view]
KO	K00772.
PANTHER	PTHR11904. Mtap_PNP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01694. MTAP. 1 hit.
PROSITE	PS01240. PNP_MTAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTIP_METJA

Accession

Primary (citable) accession number: Q60367

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program