Q60363 (PTH_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-tRNA hydrolase
Short name=PTH
EC=3.1.1.29

Gene names

Name:	pth
Synonyms:	pth2
Ordered Locus Names:	MJ0051

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. Ref.2
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00628
Subcellular location	Cytoplasm HAMAP MF_00628.
Sequence similarities	Belongs to the PTH2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 115

115

Peptidyl-tRNA hydrolase HAMAP MF_00628

PRO_0000120292

Secondary structure

1

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115

Helix Strand Turn

Details...

Beta strand

2 – 9

8

Turn

18 – 20

3

Helix

25 – 36

12

Helix

38 – 46

9

Beta strand

51 – 58

8

Helix

59 – 71

13

Beta strand

77 – 80

4

Beta strand

92 – 97

6

Helix

102 – 106

5

Sequences

	Sequence		Length	Mass (Da)
	Q60363 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: FFBC33D185B4A8A2	FASTA	115	12,670
	10 20 30 40 50 60 MKMVVVIRND LGMGKGKMVA QGGHAIIEAF LDAKRKNPRA VDEWLREGQK KVVVKVNSEK 70 80 90 100 110 ELIDIYNKAR SEGLPCSIIR DAGHTQLEPG TLTAVAIGPE KDEKIDKITG HLKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast." Rosas-Sandoval G., Ambrogelly A., Rinehart J., Wei D., Cruz-Vera L.R., Graham D.E., Stetter K.O., Guarneros G., Soell D. Proc. Natl. Acad. Sci. U.S.A. 99:16707-16712(2002) [PubMed: 12475929] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98032.1.

PIR

C64306.

RefSeq

NP_247015.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZV3	X-ray	2.10	A/B/C/D/E/F/G/H/I	1-115	[»]

ProteinModelPortal

Q60363.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1450890.

GenomeReviews

Gene locus MJ0051 in contig L77117_GR.

KEGG

mja:MJ_0051.

NMPDR

fig|243232.1.peg.52.

TIGR

MJ0051.

Phylogenomic databases

HOGENOM

HBG526257.

OMA

KSLVMEY.

ProtClustDB

PRK04322.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0051-MONOMER.

Family and domain databases

HAMAP

MF_00628. Pept_tRNA_hydro_arch.
[Tree]

InterPro

IPR023476. Pep_tRNA_hydro_II_dom.
IPR002833. Pep_tRNA_hydro_PTH2.
[Graphical view]

Gene3D

G3DSA:3.40.1490.10. G3DSA:3.40.1490.10. 1 hit.

KO

K04794.

PANTHER

PTHR12649:SF6. PTHR12649:SF6. 1 hit.

Pfam

PF01981. PTH2. 1 hit.
[Graphical view]

SUPFAM

SSF102462. SSF102462. 1 hit.

TIGRFAMs

TIGR00283. Arch_pth2. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PTH_METJA

Accession

Primary (citable) accession number: Q60363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program