Reviewed, UniProtKB/Swiss-Prot Q60362 (NAH1_METJA)

Names and origin

Protein names

Recommended name:
    Na(+)/H(+) antiporter 1
Alternative name(s):
    MjNhaP1

Gene names

Ordered Locus Names:

MJ0057

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a Na+/H+ antiporter. Can also transport lithium. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]
Biophysicochemical properties	pH dependence: Active at pH 7.0 and below.

Ontologies

Keywords
Biological process	Antiport Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW solute:hydrogen antiporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Na(+)/H(+) antiporter 1		PRO_0000052402
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	29 – 49	21	Potential
	Transmembrane	57 – 77	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	120 – 140	21	Potential
	Transmembrane	158 – 178	21	Potential
	Transmembrane	184 – 204	21	Potential
	Transmembrane	208 – 228	21	Potential
	Transmembrane	236 – 256	21	Potential
	Transmembrane	286 – 306	21	Potential
	Transmembrane	309 – 329	21	Potential
	Transmembrane	382 – 402	21	Potential
Experimental info
	Mutagenesis	93	1	D → A: Strong decrease in activity. Ref.3
	Mutagenesis	132	1	D → A: Loss of activity. Ref.3
	Mutagenesis	156	1	E → A: Almost no change in activity. Ref.3
	Mutagenesis	161	1	D → A: Loss of activity. Ref.3
	Mutagenesis	211	1	H → R: No change in activity; when associated with R-215. Ref.3
	Mutagenesis	215	1	H → R: No change in activity; when associated with R-211. Ref.3
	Mutagenesis	320	1	R → A or D: Loss of activity. Ref.3
	Mutagenesis	320	1	R → H: Almost no change in activity. Ref.3
	Mutagenesis	333	1	H → R: No change in activity. Ref.3
	Mutagenesis	347	1	R → A: Strong decrease in activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q60362-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 967F9A3E60949A1D	FASTA	426	45,966
	10 20 30 40 50 60 MELMMAIGYL GLALVLGSLV AKIAEKLKIP DIPLLLLLGL IIGPFLQIIP SDSAMEIFEY 70 80 90 100 110 120 AGPIGLIFIL LGGAFTMRIS LLKRVIKTVV RLDTITFLIT LLISGFIFNM VLNLPYTSPV 130 140 150 160 170 180 GYLFGAITAA TDPATLIPVF SRVRTNPEVA ITLEAESIFN DPLGIVSTSV ILGLFGLFSS 190 200 210 220 230 240 SNPLIDLITL AGGAIVVGLL LAKIYEKIII HCDFHEYVAP LVLGGAMLLL YVGDDLLPSI 250 260 270 280 290 300 CGYGFSGYMA VAIMGLYLGD ALFRADDIDY KYIVSFCDDL SLLARVFIFV FLGACIKLSM 310 320 330 340 350 360 LENYFIPGLL VALGSIFLAR PLGVFLGLIG SKHSFKEKLY FALEGPRGVV PAALAVTVGI 370 380 390 400 410 420 EILKNADKIP ASITKYITPT DIAGTIIIGT FMTILLSVIL EASWAGMLAL KLLGEYKPKY KEESHH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii." Hellmer J., Paetzold R., Zeilinger C. FEBS Lett. 527:245-249(2002) [PubMed: 12220668] [Abstract] Cited for: FUNCTION.
[3]	"Conserved arginine and aspartate residues are critical for function of MjNhaP1, a Na+/H+ antiporter of M. jannaschii." Hellmer J., Teubner A., Zeilinger C. FEBS Lett. 542:32-36(2003) [PubMed: 12729893] [Abstract] Cited for: MUTAGENESIS OF ASP-93; ASP-132; GLU-156; ASP-161; HIS-211; HIS-215; ARG-320; HIS-333 AND ARG-347.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98037.1.
PIR	A64307.
RefSeq	NP_247021.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450896.
GenomeReviews	Gene locus MJ0057 in contig L77117_GR.
KEGG	mja:MJ0057.
NMPDR	fig|243232.1.peg.58.
TIGR	MJ0057.
Phylogenomic databases
HOGENOM	Q60362.
OMA	SIFNDPL
Family and domain databases
InterPro	IPR006153. Cation/H_exchanger. [Graphical view]
Pfam	PF00999. Na_H_Exchanger. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAH1_METJA

Accession

Primary (citable) accession number: Q60362

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)