Reviewed, UniProtKB/Swiss-Prot Q60357 (IF6_METJA)

Names and origin

Protein names

Recommended name:
    Translation initiation factor 6
      Short name=aIF-6

Gene names

Name:	eif6
Ordered Locus Names:	MJ0048

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex By similarity.
Sequence similarities	Belongs to the eIF-6 family.
Mass spectrometry	Molecular mass is 24948±6 Da from positions 1 - 228. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Initiation factor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	mature ribosome assembly Inferred from electronic annotation. Source: InterPro translational initiation Inferred from electronic annotation. Source: HAMAP
Molecular function	ribosome binding Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Translation initiation factor 6 HAMAP MF_00032

PRO_0000153746

Secondary structure

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228

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Helix

15 – 18

4

Beta strand

23 – 28

6

Helix

34 – 44

11

Beta strand

47 – 50

4

Helix

59 – 62

4

Beta strand

67 – 73

7

Helix

78 – 90

13

Beta strand

96 – 100

5

Helix

107 – 110

4

Beta strand

111 – 113

3

Beta strand

115 – 120

6

Helix

122 – 127

6

Helix

128 – 135

8

Beta strand

137 – 141

5

Turn

150 – 152

3

Beta strand

153 – 156

4

Beta strand

158 – 163

6

Helix

169 – 179

11

Beta strand

182 – 186

5

Turn

190 – 192

3

Helix

196 – 198

3

Beta strand

200 – 202

3

Beta strand

207 – 210

4

Helix

215 – 225

11

Sequences

	Sequence		Length	Mass (Da)
	Q60357-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 94DA7E11C10FCB47	FASTA	228	24,459
	10 20 30 40 50 60 MTMIIRKYFS GIPTIGVLAL TTEEITLLPI FLDKDDVNEV SEVLETKCLQ TNIGGSSLVG 70 80 90 100 110 120 SLSVANKYGL LLPKIVEDEE LDRIKNFLKE NNLDLNVEII KSKNTALGNL ILTNDKGALI 130 140 150 160 170 180 SPELKDFKKD IEDSLNVEVE IGTIAELPTV GSNAVVTNKG CLTHPLVEDD ELEFLKSLFK 190 200 210 220 VEYIGKGTAN KGTTSVGACI IANSKGAVVG GDTTGPELLI IEDALGLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Crystal structures of ribosome anti-association factor IF6." Groft C.M., Beckmann R., Sali A., Burley S.K. Nat. Struct. Biol. 7:1156-1164(2000) [PubMed: 11101899] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

L77117 Genomic DNA. Translation: AAB98029.1.

PIR

H64305.

RefSeq

NP_247012.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G61	X-ray	1.30	A/B	1-228	[»]

ModBase

Search...

Genome annotation databases

GeneID

1450887.

GenomeReviews

Gene locus MJ0048 in contig L77117_GR.

KEGG

mja:MJ0048.

NMPDR

fig|243232.1.peg.49.

TIGR

MJ0048.

Phylogenomic databases

HOGENOM

Q60357.

OMA

QFENSNE.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0048-MON.

Family and domain databases

HAMAP

MF_00032.
[Tree]

InterPro

IPR002769. eIF6.
[Graphical view]

PANTHER

PTHR10784. eIF6. 1 hit.

Pfam

PF01912. eIF-6. 1 hit.
[Graphical view]

PIRSF

PIRSF006413. IF-6. 1 hit.

ProDom

PD006880. eIF6. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00654. eIF6. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00323. eIF-6. 1 hit.

ProtoNet

Search...

Entry information

Entry name

IF6_METJA

Accession

Primary (citable) accession number: Q60357

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)