Reviewed, UniProtKB/Swiss-Prot Q60348 (Y043_METJA)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein MJ0043
Cleaved into the following chain:
    1- Recommended name:
            Mja hyp1 intein

Gene names

Ordered Locus Names:

MJ0043

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Uncharacterized protein MJ0043, 1st part Potential		PRO_0000013988
	Chain	129 – 520	392	Mja hyp1 intein Potential		PRO_0000013989
	Chain	521 – 785	265	Uncharacterized protein MJ0043, 2nd part Potential		PRO_0000013990
Regions
	Domain	293 – 421	129	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q60348-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4167A59032511F17	FASTA	785	91,459
	10 20 30 40 50 60 MIANVDLHIH SRFSGGTSKD MNVENILKYG KLKGLNIIGT GDCTHPDYLE EIKQYKDREL 70 80 90 100 110 120 ILTTEIEDKN RVHHLILLPS ISKVEELREI LKKYSKDIDK EGRPRVSIGG AELLEIVRDV 130 140 150 160 170 180 GGLIGPAHCV PPDTLLILEN GFKRIVDIKV GDKVLTHENR FKKVEKVYKR RYIGDIIKIK 190 200 210 220 230 240 VRYFPEEIIL TPEHPVYAIK TEKRCDGSHG ICKFNCLTQY TNPSCKKRYR KYKREWIIAK 250 260 270 280 290 300 DLKVGDVIVY PIPNRVRDIK YLSLDKYLSN IKREFCRSRI PEKIEVSEEF CRLVGYFLSE 310 320 330 340 350 360 GYCFRDGIGF ALGENEKKII DDIEYLMKKI FNLKPKIRDD GRSEGIELKY YSRVLRDFFG 370 380 390 400 410 420 DMFYCGDEKR AWNKALPNEF LYLPKNKQLQ IFIGWWRGDK GVTTSEILMN QLRLISLRLG 430 440 450 460 470 480 FIITFSKHVP KNPKIGDREV IKYHARWQGR VSILDEKIVD ELKNEDIKLP KKDVRYGWIK 490 500 510 520 530 540 GNYLYAPIIR IGREYYDGFV YNLEVEDDSS YVTVSGTLHN CFTPWTSLYK SFDSIYDCYN 550 560 570 580 590 600 KKPDFVELGL SADTDMADMI PELRDLPFLS NSDAHSYHPH RLGREFNQIE VDYIGGIEDN 610 620 630 640 650 660 FEQIKKAIKH NKIIANYGLD PKLGKYHLTA CSKCHTRFKL EDAKKYNWKC PKCGGSIKKG 670 680 690 700 710 720 VLSRVEELSD GKIEHPKFRP PYYKLIPLAE MISLTIGKGI FTKAVQSLWE EFIKKYGNEI 730 740 750 760 770 780 EVLINADIDE LSKIHPKVAE TINLFRKGKI YIYPGGGGEY GKISFKPQKV EWYREEVTLD RWLKQ

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98023.1.
PIR	C64305.
RefSeq	NP_247006.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450881.
GenomeReviews	Gene locus MJ0043 in contig L77117_GR.
KEGG	mja:MJ0043.
NMPDR	fig|243232.1.peg.43.
TIGR	MJ0043.
Family and domain databases
InterPro	IPR005287. CHP00375. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. [Graphical view]
Pfam	PF02811. PHP. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00375. CHP375. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y043_METJA

Accession

Primary (citable) accession number: Q60348

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)