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Q60343 (TRM4_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (cytosine(48)-C(5))-methyltransferase
EC=2.1.1.-
Alternative name(s):
aTrm4
tRNA (cytosine-5-)-methyltransferase Trm4
Gene names
Name:trm4
Ordered Locus Names:MJ0026
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes AdoMet-dependent formation of m5C in tRNA. Cytidine residue at either position 40 or position 48 is likely to be methylated. Ref.2

Catalytic activity

S-adenosyl-L-methionine + cytosine(48) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(48) in tRNA precursor. Ref.2

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processrRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (cytosine-5-)-methyltransferase activity

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274tRNA (cytosine(48)-C(5))-methyltransferase
PRO_0000211821

Regions

Region91 – 977S-adenosyl-L-methionine binding

Sites

Active site2121Nucleophile Probable
Binding site1151S-adenosyl-L-methionine
Binding site1201S-adenosyl-L-methionine
Binding site1421S-adenosyl-L-methionine
Binding site1631S-adenosyl-L-methionine
Binding site1691S-adenosyl-L-methionine
Binding site1891S-adenosyl-L-methionine

Secondary structure

1 ................................................. 274
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q60343 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F7396827523369CF

FASTA27431,490
        10         20         30         40         50         60 
MMIVYKGEKM QFIRVNTLKI NPEVLKKRLE NKGVVLEKTF LDYAFEVKKS PFSIGSTPEY 

        70         80         90        100        110        120 
LFGYYMPQSI SSMIPPIVLN PREDDFILDM CAAPGGKTTH LAQLMKNKGT IVAVEISKTR 

       130        140        150        160        170        180 
TKALKSNINR MGVLNTIIIN ADMRKYKDYL LKNEIFFDKI LLDAPCSGNI IKDKNRNVSE 

       190        200        210        220        230        240 
EDIKYCSLRQ KELIDIGIDL LKKDGELVYS TCSMEVEENE EVIKYILQKR NDVELIIIKA 

       250        260        270 
NEFKGINIKE GYIKGTLRVF PPNEPFFIAK LRKI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L77117 Genomic DNA. Translation: AAB98007.1.
PIRB64303.
RefSeqNP_246989.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3A4TX-ray2.30A/B1-274[»]
3AJDX-ray1.27A1-274[»]
ProteinModelPortalQ60343.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1450864.
GenomeReviewsGene locus MJ0026 in contig L77117_GR.
KEGGmja:MJ_0026.
NMPDRfig|243232.1.peg.26.
TIGRMJ0026.

Phylogenomic databases

HOGENOMHBG726909.
OMANEPFFIA.
ProtClustDBCLSK876038.

Enzyme and pathway databases

BioCycMJAN243232:MJ_0026-MONOMER.

Family and domain databases

InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
[Graphical view]
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
TIGRFAMsTIGR00446. Nop2p. 1 hit.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRM4_METJA
AccessionPrimary (citable) accession number: Q60343
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 19, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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