Reviewed, UniProtKB/Swiss-Prot Q60341 (FRHB_METJA)

Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit beta
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
      Short name=FRH

Gene names

Name:	frhB
Ordered Locus Names:	MJ0032

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen By similarity.
Catalytic activity	H(2) + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel By similarity. Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH By similarity. FAD By similarity.
Subunit structure	Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8) By similarity.
Sequence similarities	Belongs to the frhB family.

Ontologies

Keywords
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Coenzyme F420 hydrogenase subunit beta		PRO_0000159226

Sequences

	Sequence		Length	Mass (Da)
	Q60341-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 7CE7D4E6E970940E	FASTA	287	32,054
	10 20 30 40 50 60 MRWNRMNPFG SYKKVVSARS TLKEVLKKAQ DGGIVSTAFI YGLENNLLDG VIVADNAGEF 70 80 90 100 110 120 KAVPKVATTP EEVLEAAGTK YTVCPNISVL KSAVREYGCE KIGVVGTPCQ VRAVRKLMKY 130 140 150 160 170 180 PVGFRHVPDK IALIIGIFCM ENFPYYGLKL IVEEHCGVKM EDVVKMDIGK GKFWVYTRWG 190 200 210 220 230 240 ETKAIKLKET HPYEQIACHV CTDYTAELAD ISTGSVGSPD GWSTVFIRTA KGEEIFNKMV 250 260 270 280 EDGYLEVKPI EEVKPGLGLV EKLALQKKEK NMKEIEHRKE LGLPIPY

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98011.1.
PIR	H64303.
RefSeq	NP_246995.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450870.
GenomeReviews	Gene locus MJ0032 in contig L77117_GR.
KEGG	mja:MJ0032.
NMPDR	fig|243232.1.peg.32.
TIGR	MJ0032.
Phylogenomic databases
HOGENOM	Q60341.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0032-MON.
Family and domain databases
InterPro	IPR007516. Co_F420_Hydgase/DHase_bsu_N. IPR017679. Coenz_F4210_hydrogenase_bsu. IPR007525. FrhB_FdhB_C. [Graphical view]
Pfam	PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q60341.

Entry information

Entry name

FRHB_METJA

Accession

Primary (citable) accession number: Q60341

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 4, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)