Reviewed, UniProtKB/Swiss-Prot Q60340 (FRHG_METJA)

Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit gamma
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
      Short name=FRH

Gene names

Name:	frhG
Ordered Locus Names:	MJ0031

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen By similarity.
Catalytic activity	H(2) + coenzyme F420 = reduced coenzyme F420.
Subunit structure	Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8) By similarity.
Sequence similarities	Belongs to the frhG family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Coenzyme F420 hydrogenase subunit gamma		PRO_0000159232
Regions
	Domain	169 – 197	29	4Fe-4S ferredoxin-type 1
	Domain	198 – 228	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	178	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	181	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	184	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	188	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	207	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	210	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	213	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	217	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60340-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6C9936D3A20ED356	FASTA	230	24,534
	10 20 30 40 50 60 MIKVVKVAHV QLCSCCGCLV SLADTYEKLL DVLNSIELVY CQTLADAREI PECDIALVEG 70 80 90 100 110 120 SVCLDDHHSL EVAQEVRKKA KIVVALGACA ATGGVTRYCK GNQLSKPVHS SFSPLTEVIK 130 140 150 160 170 180 VDLAIPGCPP SPEAIVGVIT AALNGDMEYL QPYAELAEKG SEACGCDVIY KVVNKSLCMG 190 200 210 220 230 CGTCAAACPT RAIEMLDGRP NVLKELCIKC GACSVQCPRI RFPELIEKIE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98010.1.
PIR	G64303.
RefSeq	NP_246994.1.
3D structure databases
HSSP	HSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBase	Search...
Genome annotation databases
GeneID	1450869.
GenomeReviews	Gene locus MJ0031 in contig L77117_GR.
KEGG	mja:MJ0031.
NMPDR	fig|243232.1.peg.31.
TIGR	MJ0031.
Phylogenomic databases
HOGENOM	Q60340.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0031-MON.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR017681. Coenz_F4210_hydrogenase_gsu. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR001821. NiFe_hyd_ssu. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF00037. Fer4. 2 hits. PF01058. Oxidored_q6. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN. PR00614. NIHGNASESMLL.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHG_METJA

Accession

Primary (citable) accession number: Q60340

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)