Q60340 (FRHG_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme F420 hydrogenase subunit gamma
EC=1.12.98.1

Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name=FRH

Gene names

Name:	frhG
Ordered Locus Names:	MJ0031

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen By similarity.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Subunit structure	Heterocomplex of the form (alpha1beta1gamma1)8 By similarity.
Sequence similarities	Belongs to the FrhG family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Coenzyme F420 hydrogenase subunit gamma		PRO_0000159232
Regions
	Domain	169 – 197	29	4Fe-4S ferredoxin-type 1
	Domain	198 – 228	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	178	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	181	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	184	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	188	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	207	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	210	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	213	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	217	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60340 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6C9936D3A20ED356	FASTA	230	24,534
	10 20 30 40 50 60 MIKVVKVAHV QLCSCCGCLV SLADTYEKLL DVLNSIELVY CQTLADAREI PECDIALVEG 70 80 90 100 110 120 SVCLDDHHSL EVAQEVRKKA KIVVALGACA ATGGVTRYCK GNQLSKPVHS SFSPLTEVIK 130 140 150 160 170 180 VDLAIPGCPP SPEAIVGVIT AALNGDMEYL QPYAELAEKG SEACGCDVIY KVVNKSLCMG 190 200 210 220 230 CGTCAAACPT RAIEMLDGRP NVLKELCIKC GACSVQCPRI RFPELIEKIE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98010.1.
PIR	G64303.
RefSeq	NP_246994.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60340.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98010; AAB98010; MJ_0031.
GeneID	1450869.
KEGG	mja:MJ_0031.
Phylogenomic databases
eggNOG	COG1941.
KO	K00443.
OMA	CPPSPEI.
ProtClustDB	CLSK876042.
Family and domain databases
Gene3D	3.40.50.700. 1 hit.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017681. Coenz_F420_hydrogenase_gsu. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB. IPR001821. NiFe_hydrogenase_ssu. [Graphical view]
PANTHER	PTHR11995. PTHR11995. 1 hit.
Pfam	PF00037. Fer4. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR03294. FrhG. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHG_METJA

Accession

Primary (citable) accession number: Q60340

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program