Q60338 (FRHA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme F420 hydrogenase subunit alpha
EC=1.12.98.1

Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha
Short name=FRH

Gene names

Name:	frhA
Ordered Locus Names:	MJ0029

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen By similarity.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel By similarity. Iron-sulfur. There are 12-13 Fe atoms/(alpha1beta1gamma1) unit of the FRH By similarity. FAD By similarity.
Subunit structure	Heterocomplex of the form (alpha1beta1gamma1)8 By similarity.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	FAD Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Coenzyme F420 hydrogenase subunit alpha		PRO_0000199722
Sites
	Metal binding	68	1	Nickel Potential
	Metal binding	71	1	Nickel Potential
	Metal binding	391	1	Nickel Potential
	Metal binding	394	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60338 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8DD9A94CBABE35C4	FASTA	415	46,784
	10 20 30 40 50 60 MEVNFVTNRI EIAPTTRHEG HAKLILEVDE EGIVNKAYYL NTTPVRGFET MLKGKPAEFA 70 80 90 100 110 120 PIAVMRICGI CQTTHGIASC EAIENAIDCE VPDDGLLLRE LVGIGNRLHS HPLHHLLTID 130 140 150 160 170 180 DFLKPDETDL KIELIKLIQR MRKVGQLVVD IVGGEGIHPP NIVIGGMRTN ITERAKSRLY 190 200 210 220 230 240 YALRQYEKDA YELYEKYTEL IERYLEEIGI PDLGAHEYPY IATHTTYGDR YAINWDDVTE 250 260 270 280 290 300 IPAQRYYDDE EAKQTTTIQI PLYAGVPAEG GPRARMVKFG NFREGGSAMD INIARAQENL 310 320 330 340 350 360 GAVYRALEIL DELDLNGKTR AEVEYKDGFG IGVHEAPRAT NTHMAEVGKD GKIKSYRIIA 370 380 390 400 410 ASTWNFPIVE KAIEGYPQQY AEVIMRAYDI CASCATHVIV KDEETKEIIE VRKML

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. No translation available.
PIR	A64299.
3D structure databases
ProteinModelPortal	Q60338.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus MJ0029 in contig L77117_GR.
TIGR	MJ0029.
Phylogenomic databases
HOGENOM	HBG287963.
OMA	DIXASCA.
Family and domain databases
InterPro	IPR017682. Coenz_F420_hydrogenase_asu. IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
TIGRFAMs	TIGR03295. FrhA. 1 hit.
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHA_METJA

Accession

Primary (citable) accession number: Q60338

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program