Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPBy similarity1
Active sitei304Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi280 – 284ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-26-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:MJ0025
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564261 – 338RNA 3'-terminal phosphate cyclaseAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0025

Structurei

3D structure databases

ProteinModelPortaliQ60335
SMRiQ60335
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
InParanoidiQ60335
KOiK01974
OMAiANRPKPG
OrthoDBiPOG093Z05RD
PhylomeDBiQ60335

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q60335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFIVIDGSY LEGGGQIIRT AVSLSALTQK PVKIINIRKK RKNKGLAPQH
60 70 80 90 100
VSAVKAVKKL CNAEVFGLNV GSEELTFIPS KLSPKDFTID IGTAGSISLV
110 120 130 140 150
IQTLLPLSLG INKKFTVKIK GGTDVKRAPP IDYVKNVTLK ILRNFGVLTE
160 170 180 190 200
LKVLKRGFYP EGGGEVIFEV KPSKIKKFDL IEHSKSNLVE GISYVQNLDE
210 220 230 240 250
SIARRMRKKA VDLLNKEKLL PNIKIECSKG ISTGAGIVLW NDTLGGSCLG
260 270 280 290 300
EKGLRAEIVA ERAVNELLKE RESGMALDKY MGDQIIPFLA FGKGIVGVSE
310 320 330
ITNHTKTNMW VVKHFLDVDF EIKEYKENNC NGFTIEVV
Length:338
Mass (Da):37,229
Last modified:November 1, 1997 - v1
Checksum:i4681A2AB120FD6F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98006.1
PIRiA64303
RefSeqiWP_010869517.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB98006; AAB98006; MJ_0025
GeneIDi1450863
KEGGimja:MJ_0025

Similar proteinsi

Entry informationi

Entry nameiRTCA_METJA
AccessioniPrimary (citable) accession number: Q60335
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health