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Q60335 (RTCA_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:MJ0025
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000156426

Regions

Nucleotide binding280 – 2845ATP By similarity

Sites

Active site3041Tele-AMP-histidine intermediate By similarity
Binding site1021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60335 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4681A2AB120FD6F0

FASTA33837,229
        10         20         30         40         50         60 
MDFIVIDGSY LEGGGQIIRT AVSLSALTQK PVKIINIRKK RKNKGLAPQH VSAVKAVKKL 

        70         80         90        100        110        120 
CNAEVFGLNV GSEELTFIPS KLSPKDFTID IGTAGSISLV IQTLLPLSLG INKKFTVKIK 

       130        140        150        160        170        180 
GGTDVKRAPP IDYVKNVTLK ILRNFGVLTE LKVLKRGFYP EGGGEVIFEV KPSKIKKFDL 

       190        200        210        220        230        240 
IEHSKSNLVE GISYVQNLDE SIARRMRKKA VDLLNKEKLL PNIKIECSKG ISTGAGIVLW 

       250        260        270        280        290        300 
NDTLGGSCLG EKGLRAEIVA ERAVNELLKE RESGMALDKY MGDQIIPFLA FGKGIVGVSE 

       310        320        330 
ITNHTKTNMW VVKHFLDVDF EIKEYKENNC NGFTIEVV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98006.1.
PIRA64303.
RefSeqNP_246988.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ60335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0025.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98006; AAB98006; MJ_0025.
GeneID1450863.
KEGGmja:MJ_0025.

Phylogenomic databases

eggNOGCOG0430.
KOK01974.
OMAAEMLLRN.
PhylomeDBQ60335.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_METJA
AccessionPrimary (citable) accession number: Q60335
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names