Reviewed, UniProtKB/Swiss-Prot Q60331 (GATD_METJA)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA(Gln) amidotransferase subunit D
      Short name=Glu-ADT subunit D
    EC=6.3.5.-

Gene names

Name:	gatD
Ordered Locus Names:	MJ0020

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The gatDE system is specific for glutamate and does not act on aspartate By similarity.
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00586
Subunit structure	Heterodimer of gatD and gatE By similarity.
Sequence similarities	Belongs to the asparaginase 1 family. GatD subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparaginase activity Inferred from electronic annotation. Source: InterPro glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Glutamyl-tRNA(Gln) amidotransferase subunit D HAMAP MF_00586		PRO_0000140052
Sites
	Active site	84	1	By similarity
	Active site	160	1	By similarity
	Active site	161	1	By similarity
	Active site	237	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60331-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 27FFF41E62F41EE3	FASTA	417	46,344
	10 20 30 40 50 60 MNVGDVIRVE TDKGIFEGIL LPSTNENIIT IKMKNGYNVG ILKENVKNIE IIAKGEKPKY 70 80 90 100 110 120 ELPPLNIEKN EKLKTISILS TGGTVASKVD YKTGAVHPSF TADDLIRAVP ELLDIANIKG 130 140 150 160 170 180 RAVMNILSEN MKPEYWRKIA EEIKKEIEEG ADGIVIAHGT DTMSYTASAL SFMVKADVPI 190 200 210 220 230 240 ILVGAQRSSD RPSSDAALNL ISAVLAAREP IKGVYVVMHG ESGDTFCYLH KGVKVRKCHS 250 260 270 280 290 300 SRRDAFKSIN SIPVAKINPF TKEIIYLQEV EKSDNSKKVE INTNLEEKVA LIKVYPGMDG 310 320 330 340 350 360 EIIRFYVDKE YKGIVLEGTG LGHAPEYIFE HIKYATDKGV VVVMTTQTIN GRVNMNVYSN 370 380 390 400 410 GRELQKLGVI GCEDMPPEVA LVKLMYLLGN YEPEEVKKLI NKNLVGEIEY RSRFDAY

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB97996.1.
PIR	D64302.
RefSeq	NP_246983.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450858.
GenomeReviews	Gene locus MJ0020 in contig L77117_GR.
KEGG	mja:MJ0020.
NMPDR	fig|243232.1.peg.20.
TIGR	MJ0020.
Phylogenomic databases
HOGENOM	Q60331.
OMA	PEFYHDE
Family and domain databases
HAMAP	MF_00586. [Tree]
InterPro	IPR006033. AsnASEI. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. IPR011878. GatD_arch. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00519. asnASE_I. 1 hit. TIGR02153. gatD_arch. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATD_METJA

Accession

Primary (citable) accession number: Q60331

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)