Reviewed, UniProtKB/Swiss-Prot Q60325 (GATE_METJA)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA(Gln) amidotransferase subunit E
      Short name=Glu-ADT subunit E
    EC=6.3.5.-

Gene names

Name:	gatE
Ordered Locus Names:	MJ0019

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The gatDE system is specific for glutamate and does not act on aspartate By similarity.
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00588
Subunit structure	Heterodimer of gatD and gatE By similarity.
Sequence similarities	Belongs to the gatB/gatE family. GatE subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	Glutamyl-tRNA(Gln) amidotransferase subunit E HAMAP MF_00588		PRO_0000140072

Sequences

	Sequence		Length	Mass (Da)
	Q60325-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 43D401B61B88F7AF	FASTA	630	71,225
	10 20 30 40 50 60 MEINYEKVGL KVGLEIHQQL NTKRKLFCHC PTILRDDEPD GEIVRVLRPS LSEMGEVDRA 70 80 90 100 110 120 ALIEARKGKK FIYQFYNDTT CLVELDEEPP HPPSEEALRI ALEVALLMNM NVVDVAYTMR 130 140 150 160 170 180 KIVIDGSNTS GFQRTIFLAR DGYIETSEGK VGITSLCLEE DAARKIEDRG DAVVYNLDRL 190 200 210 220 230 240 GIPLVEISTA PDIKTPKMAK EAARRIGEIL RATGKVKRGL GTIRQDINIS IKDGARIEVK 250 260 270 280 290 300 GVQDLDLIEK VVENEVIRQL NLLKIRDELR ERNAEVVEKI FDVTEIFKDC KSKIIQNALK 310 320 330 340 350 360 KKNGKVKAVL LKGFAGLVGK EIQPGRRLGT EFSDRAKVIA GVGGLFHTDE LPKYGITEEE 370 380 390 400 410 420 VKKLKEFVNA EENDAVIIVA DEESKVDRAL EAVIERAKEA LIGVPEETRR ALEDGNTAYL 430 440 450 460 470 480 RPLPGAARMY PETDIPPIII KKEFIEEIRA NLPELPEEKF ERFKKEYKLN DELAKKMVLS 490 500 510 520 530 540 YYVDLFEDLC KKFKNVKPVL IATTLEGTLK EIKREGYDID KLEDRHLEET FKALSEGKIA 550 560 570 580 590 600 KEGIVEVLKG FCEFPDKSID EILEIKGLKG LSKEEVEKII EGIIKEHLNV VKEKGEKAYG 610 620 630 FLMGRCMAKL RGKADGKLVN DILRKKLKEI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB97995.1.
PIR	C64302.
RefSeq	NP_246982.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450857.
GenomeReviews	Gene locus MJ0019 in contig L77117_GR.
KEGG	mja:MJ0019.
NMPDR	fig|243232.1.peg.19.
TIGR	MJ0019.
Phylogenomic databases
HOGENOM	Q60325.
OMA	TSGFQRT
Family and domain databases
HAMAP	MF_00588. [Tree]
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR004115. GAD_dom. IPR004414. Gln-tRNA_amidoTrfase_esu. IPR006107. Gln-tRNA_amidoTrfase_suB/E_cen. IPR006075. Gln-tRNA_amidoTrfase_suB/E_N. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF01162. GatB. 1 hit. PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
TIGRFAMs	TIGR00134. gatE_arch. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATE_METJA

Accession

Primary (citable) accession number: Q60325

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)