Q60301 (Y3402_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized adenine-specific methylase MJECS02
EC=2.1.1.72

Gene names

Ordered Locus Names:

MJECS02

Encoded on

Plasmid small ECE

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	1181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1181	1181	Uncharacterized adenine-specific methylase MJECS02		PRO_0000088002

Sequences

	Sequence		Length	Mass (Da)
	Q60301 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9F961D8F8C6A4BDD	FASTA	1,181	138,439
	10 20 30 40 50 60 MVVMAQTKLC SDIEIPKEVY NKWKDFIKLV GNIILSIKQI PELEGKFKEL LKKGRYNFKS 70 80 90 100 110 120 DDFGGQLPEP FTRQKVIEPI LEFLGYEFTS EISKKSPLGD RKIPDYRVSV FNKEILIEAE 130 140 150 160 170 180 PLGSDLNKKD SGIHQVKEWL IIKSYGVDTG IATNGLEWVL LHYDDTIKEI RTLKELNLKS 190 200 210 220 230 240 IFEYVLENKK DKDLENELKQ VFSEFYYCFS KEYIEEYIEV ATKNIKHKKE EITNEFYKEF 250 260 270 280 290 300 VKLVFGFEDV KDVKKKDKSS SEKDKGTKKC LYNCIEAPPN TSELDKKKFA VLLMNRLIFI 310 320 330 340 350 360 KFLEDKGIVP RDLLRRTYED YKKSNVLINY YDAYLKPLFY EVLNTPEDER KENIRTNPYY 370 380 390 400 410 420 KDIPYLNGGL FRSNNVPNEL SFTIKDNEII GEVINFLERY KFTLSTSEGS EEVELNPDIL 430 440 450 460 470 480 GYVYEKLINI LAEKGQKGLG AYYTPDEITS YIAKNTIEPI VVERFKEIIK NWKINDINFS 490 500 510 520 530 540 TLDEILNEDS KIAENKHILR AFLDELDKIR ILDPAVGSGH FLISALKELL QIKKRIYYLL 550 560 570 580 590 600 REEMDIYKEK LGIILNNLYG VDIDDIAVEI AKLRLWLALI ENLDVEALKR GEVLLPNIEY 610 620 630 640 650 660 NVRCGNSLVG WIDENLKQLS ISYLCDNVRI MCVLEGLIIN AHNSEERKKL KKAKELLEKR 670 680 690 700 710 720 DGYVLDNYVE AYHLLYEVYR TSHGLKANLL KELLDEIRDS IYESVTPAYF AEIYQNGNNK 730 740 750 760 770 780 KNNGKKSKKN RPRVEEFEKL KPFHWKIDFG WIIKEEGFDV IIGNPPYGNL LSPTEKEIMK 790 800 810 820 830 840 RRDTPEFDIF VTFIVHSSKL LKNEGYLGFI IPSSFGTGVR YSNLRKELFT KMCLKKLIYL 850 860 870 880 890 900 PFDVFSGAYV DNCIIILHKK PPKSEDLVLI YAFPKKTKKI SFEFKNDLFI EYSKILNDPK 910 920 930 940 950 960 CRIFPKSPEI YIILDKIKQN CRESLTYLED LTESTIGILA SKYKFSDKKE NEYYLPYLEG 970 980 990 1000 1010 1020 NVYRYETKLK LKNYVDFSKH KNNEKLINLF MSPEKIFIRR IVNRQDRIMA SYGNIEGVVK 1030 1040 1050 1060 1070 1080 KDLYVFVLKP DTPINYFYLL GILNSELISY IYIGKSAIAL KDDFRQTTLE ELRELPIVIP 1090 1100 1110 1120 1130 1140 KNKNIINALT QLSKLRFELN DKLNENDRIF LENIIDSLVY GIYFQDLIPK EELNEICNEI 1150 1160 1170 1180 NGIIKKYDEK SIDCLKYCQN IIKLLNTINT NELVRKIRNK N

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77119 Genomic DNA. Translation: AAC37060.1.
PIR	B64516.
RefSeq	NP_044172.1. NC_001733.1.
3D structure databases
ProteinModelPortal	Q60301.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJECS02.
Protein family/group databases
REBASE	6813. MjaORFECS2P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC37060; AAC37060; MJ_ECS02.
GeneID	1450829.
KEGG	mja:MJECS02.
Phylogenomic databases
eggNOG	COG1002.
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR011639. RM_methylase_Eco57I. IPR025931. TaqI_C. [Graphical view]
Pfam	PF07669. Eco57I. 1 hit. PF02384. N6_Mtase. 1 hit. PF12950. TaqI_C. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y3402_METJA

Accession

Primary (citable) accession number: Q60301

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program