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Q602B7

- SYI_MYCH2

UniProt

Q602B7 - SYI_MYCH2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, mhp033
Organism
Mycoplasma hyopneumoniae (strain 232)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei546 – 5461Aminoacyl-adenylate By similarity
Binding sitei590 – 5901ATP By similarity
Metal bindingi856 – 8561Zinc By similarity
Metal bindingi859 – 8591Zinc By similarity
Metal bindingi870 – 8701Zinc By similarity
Metal bindingi873 – 8731Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHYO295358:GH8B-33-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:mhp033
OrganismiMycoplasma hyopneumoniae (strain 232)
Taxonomic identifieri295358 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000006822: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098418Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi295358.mhp033.

Structurei

3D structure databases

ProteinModelPortaliQ602B7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi587 – 5915"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q602B7-1 [UniParc]FASTAAdd to Basket

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MDKNFYKNSL NIFNSNFSMK ANLSEKDKFY ADFWEKNQIY QQILRKRRGN    50
PRFILHDGPP YANGDIHIGH ALNKILKDII VRYKTMAGFY SPFVPGWDTH 100
GLPIENKIIN QIGSKSTLEI RRKSNDFANS QILAQMKQFK KLNLLTDFKQ 150
IYQTNTPNYE AKQLKLFKKM VSRGLVYRAL KPVYWSPSSQ SALAEAEIEY 200
LEYRSPSLFT SFDIKKGNNF VAENDKLIIW TTTPWTLIAN SGVAVGENFD 250
YVRIKNGENF YVLAANLLEK LAVIFDWKHY EIIDNFPGKS ILGIKYLHPI 300
FEKICPIVSG NHVSLDVGSG LVHLAPLFGE DDYWIGRENN LEMVMHVNDD 350
GKFNENAGQF SGQFYADSNK LITEFLEKKS KILHLSFIDH SFPHDWRTLK 400
PVIYRGTPQW FVSIEKIKKD LEKAIEEIEF PENWLKKRLT KMVVERKDWL 450
ISRQRSWGIP LIIFYDQNKE PVLDKPEIFD YIISLVEKFG SRIWYEKTTD 500
ELLPEKYQNL GWTKENDILD VWFDSGVSFF AANISDEKPP FDIYFEGSDQ 550
YRGWFNSSLI NSVIYFGFSP YKKLLSHGFV VDAKGNKMSK SRGNGVDPLV 600
ILSKYGCDIF RLWVANSEYY NDIVYSEAIF EQNVEIYRKI RNTVRFLITN 650
LADFKPKKYE LTEVDLYIFN KIQKLKNEII QNYDQNRFVR VVKIINNFII 700
EFSNFYLSIV KDILYADKEE SLKRRQVQYN LYELLQVLNI AIAPIMPTTA 750
EEIYSFIQKN NKQISVHMEE FFKKSHFDEE LDAKWDEFFQ IKDSVYQLIE 800
QKIKSKEIKR PNEVGVLLKT DSDFIKSIDL EKLLMVAKVE FSNEKTEILQ 850
LNWEKCPRCW NHFEKINKVC ARCFEVLNEI VPEKNS 886
Length:886
Mass (Da):104,166
Last modified:November 23, 2004 - v1
Checksum:i64AD4D3BBF79306A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017332 Genomic DNA. Translation: AAV27692.1.
RefSeqiWP_011205872.1. NC_006360.1.
YP_115548.1. NC_006360.1.

Genome annotation databases

EnsemblBacteriaiAAV27692; AAV27692; mhp033.
GeneIDi3105907.
KEGGimhy:mhp033.
PATRICi20010607. VBIMycHyo77580_0034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017332 Genomic DNA. Translation: AAV27692.1 .
RefSeqi WP_011205872.1. NC_006360.1.
YP_115548.1. NC_006360.1.

3D structure databases

ProteinModelPortali Q602B7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 295358.mhp033.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV27692 ; AAV27692 ; mhp033 .
GeneIDi 3105907.
KEGGi mhy:mhp033.
PATRICi 20010607. VBIMycHyo77580_0034.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MHYO295358:GH8B-33-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis."
    Minion F.C., Lefkowitz E.J., Madsen M.L., Cleary B.J., Swartzell S.M., Mahairas G.G.
    J. Bacteriol. 186:7123-7133(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 232.

Entry informationi

Entry nameiSYI_MYCH2
AccessioniPrimary (citable) accession number: Q602B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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