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Q60297

- T1MH_METJA

UniProt

Q60297 - T1MH_METJA

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Protein

Putative type I restriction enzyme MjaXP M protein

Gene

MJECL42

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei283 – 2831S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3910. M.MjaORFCL42P.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type I restriction enzyme MjaXP M protein (EC:2.1.1.72)
Short name:
M.MjaXP
Gene namesi
Ordered Locus Names:MJECL42
Encoded oniPlasmid large ECE0 Publication
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Plasmid large ECE

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Putative type I restriction enzyme MjaXP M proteinPRO_0000088029Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

STRINGi243232.MJECL42.

Structurei

3D structure databases

ProteinModelPortaliQ60297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 2326S-adenosyl-L-methionine bindingBy similarity
Regioni256 – 2583S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0286.
HOGENOMiHOG000239473.
InParanoidiQ60297.
KOiK03427.
OMAiDIAGFCK.
PhylomeDBiQ60297.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60297-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATLDKFLSI KENDEKTKKK ESKKKSSKSN KTSESLVSHD HFELTPEFEN
60 70 80 90 100
QLWKVADKLR KKMEVHQYKY VVLGLIFLRA LTCRFYERRK EIEEELSNPN
110 120 130 140 150
SELYTEDPEL RKMILEDEDF YLSEGVLYLP KETRWDYFVE NVMSPNIGEI
160 170 180 190 200
IDTAIEILEE KYPDRLKDVI PKIYAQSPLD NHDYSYLINK FSEISFGKEH
210 220 230 240 250
RVKDVFGRIY EYFLGKFTEV EGKLGGKFYT PRSLTKLIVD VLDVKGGSIF
260 270 280 290 300
DPACGSGGFF VSALEKLERE GIDINELSIY GQDSDPMAYR LTKMNLIIRG
310 320 330 340 350
AEGDIRIDDS YHDDKFMDMT FDYVVANPPF NDSEWDANRI KPDDPRLRIG
360 370 380 390 400
NKKVPVPPNG NANYMWILHF IYHTAPNGKA GFVMANGALS AGNVEGEIRK
410 420 430 440 450
AIIENDLVYG IVACPPKLFY NVSLPVSLWF IRKEKPDYMK GKVLFINAKN
460 470 480 490 500
LYKQISRRQN ILTEEHIKKI VDKFRMFESG EDEDKINELG FAKVATIDEI
510 520 530 540 550
AKNGYVLTPG RYVGVKIEDD GIPFEVKMKE YSEELKKLLD EEEKLRNKVK

EILDALGF
Length:558
Mass (Da):64,552
Last modified:November 1, 1997 - v1
Checksum:i0DD8568C71C3AF37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77118 Genomic DNA. Translation: AAC37111.1.
PIRiA64515.
RefSeqiNP_044168.1. NC_001732.1.

Genome annotation databases

EnsemblBacteriaiAAC37111; AAC37111; MJ_ECL42.
GeneIDi1450824.
KEGGimja:MJECL42.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77118 Genomic DNA. Translation: AAC37111.1 .
PIRi A64515.
RefSeqi NP_044168.1. NC_001732.1.

3D structure databases

ProteinModelPortali Q60297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJECL42.

Protein family/group databases

REBASEi 3910. M.MjaORFCL42P.

Protocols and materials databases

DNASUi 1450824.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC37111 ; AAC37111 ; MJ_ECL42 .
GeneIDi 1450824.
KEGGi mja:MJECL42.

Phylogenomic databases

eggNOGi COG0286.
HOGENOMi HOG000239473.
InParanoidi Q60297.
KOi K03427.
OMAi DIAGFCK.
PhylomeDBi Q60297.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiT1MH_METJA
AccessioniPrimary (citable) accession number: Q60297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3