Q60297 (T1MH_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type I restriction enzyme MjaXP M protein
Short name=M.MjaXP
EC=2.1.1.72

Gene names

Ordered Locus Names:

MJECL42

Encoded on

Plasmid large ECE

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Putative type I restriction enzyme MjaXP M protein		PRO_0000088029
Regions
	Region	227 – 232	6	S-adenosyl-L-methionine binding By similarity
	Region	256 – 258	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	283	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60297 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0DD8568C71C3AF37	FASTA	558	64,552
	10 20 30 40 50 60 MATLDKFLSI KENDEKTKKK ESKKKSSKSN KTSESLVSHD HFELTPEFEN QLWKVADKLR 70 80 90 100 110 120 KKMEVHQYKY VVLGLIFLRA LTCRFYERRK EIEEELSNPN SELYTEDPEL RKMILEDEDF 130 140 150 160 170 180 YLSEGVLYLP KETRWDYFVE NVMSPNIGEI IDTAIEILEE KYPDRLKDVI PKIYAQSPLD 190 200 210 220 230 240 NHDYSYLINK FSEISFGKEH RVKDVFGRIY EYFLGKFTEV EGKLGGKFYT PRSLTKLIVD 250 260 270 280 290 300 VLDVKGGSIF DPACGSGGFF VSALEKLERE GIDINELSIY GQDSDPMAYR LTKMNLIIRG 310 320 330 340 350 360 AEGDIRIDDS YHDDKFMDMT FDYVVANPPF NDSEWDANRI KPDDPRLRIG NKKVPVPPNG 370 380 390 400 410 420 NANYMWILHF IYHTAPNGKA GFVMANGALS AGNVEGEIRK AIIENDLVYG IVACPPKLFY 430 440 450 460 470 480 NVSLPVSLWF IRKEKPDYMK GKVLFINAKN LYKQISRRQN ILTEEHIKKI VDKFRMFESG 490 500 510 520 530 540 EDEDKINELG FAKVATIDEI AKNGYVLTPG RYVGVKIEDD GIPFEVKMKE YSEELKKLLD 550 EEEKLRNKVK EILDALGF

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77118 Genomic DNA. Translation: AAC37111.1.
PIR	A64515.
RefSeq	NP_044168.1. NC_001732.1.
3D structure databases
ProteinModelPortal	Q60297.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJECL42.
Protein family/group databases
REBASE	3910. M.MjaORFCL42P.
Protocols and materials databases
DNASU	1450824.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC37111; AAC37111; MJ_ECL42.
GeneID	1450824.
KEGG	mja:MJECL42.
Phylogenomic databases
eggNOG	COG0286.
HOGENOM	HOG000239473.
KO	K03427.
OMA	YGQESNY.
ProtClustDB	CLSK2517092.
Family and domain databases
InterPro	IPR022749. D12N6_MeTrfase_N. IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF12161. HsdM_N. 1 hit. PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1MH_METJA

Accession

Primary (citable) accession number: Q60297

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program