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Protein

Putative type I restriction enzyme MjaXP M protein

Gene

MJECL42

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei283 – 2831S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3910. M.MjaORFCL42P.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type I restriction enzyme MjaXP M protein (EC:2.1.1.72)
Short name:
M.MjaXP
Gene namesi
Ordered Locus Names:MJECL42
Encoded oniPlasmid large ECE0 Publication
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Plasmid large ECE

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Putative type I restriction enzyme MjaXP M proteinPRO_0000088029Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Structurei

3D structure databases

ProteinModelPortaliQ60297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 2326S-adenosyl-L-methionine bindingBy similarity
Regioni256 – 2583S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0286.
HOGENOMiHOG000239473.
InParanoidiQ60297.
KOiK03427.
OMAiLAENIFW.
PhylomeDBiQ60297.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLDKFLSI KENDEKTKKK ESKKKSSKSN KTSESLVSHD HFELTPEFEN
60 70 80 90 100
QLWKVADKLR KKMEVHQYKY VVLGLIFLRA LTCRFYERRK EIEEELSNPN
110 120 130 140 150
SELYTEDPEL RKMILEDEDF YLSEGVLYLP KETRWDYFVE NVMSPNIGEI
160 170 180 190 200
IDTAIEILEE KYPDRLKDVI PKIYAQSPLD NHDYSYLINK FSEISFGKEH
210 220 230 240 250
RVKDVFGRIY EYFLGKFTEV EGKLGGKFYT PRSLTKLIVD VLDVKGGSIF
260 270 280 290 300
DPACGSGGFF VSALEKLERE GIDINELSIY GQDSDPMAYR LTKMNLIIRG
310 320 330 340 350
AEGDIRIDDS YHDDKFMDMT FDYVVANPPF NDSEWDANRI KPDDPRLRIG
360 370 380 390 400
NKKVPVPPNG NANYMWILHF IYHTAPNGKA GFVMANGALS AGNVEGEIRK
410 420 430 440 450
AIIENDLVYG IVACPPKLFY NVSLPVSLWF IRKEKPDYMK GKVLFINAKN
460 470 480 490 500
LYKQISRRQN ILTEEHIKKI VDKFRMFESG EDEDKINELG FAKVATIDEI
510 520 530 540 550
AKNGYVLTPG RYVGVKIEDD GIPFEVKMKE YSEELKKLLD EEEKLRNKVK

EILDALGF
Length:558
Mass (Da):64,552
Last modified:November 1, 1997 - v1
Checksum:i0DD8568C71C3AF37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77118 Genomic DNA. Translation: AAC37111.1.
PIRiA64515.
RefSeqiNP_044168.1. NC_001732.1.
WP_010890090.1. NC_001732.1.

Genome annotation databases

EnsemblBacteriaiAAC37111; AAC37111; MJ_ECL42.
GeneIDi1450824.
KEGGimja:MJECL42.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77118 Genomic DNA. Translation: AAC37111.1.
PIRiA64515.
RefSeqiNP_044168.1. NC_001732.1.
WP_010890090.1. NC_001732.1.

3D structure databases

ProteinModelPortaliQ60297.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3910. M.MjaORFCL42P.

Protocols and materials databases

DNASUi1450824.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC37111; AAC37111; MJ_ECL42.
GeneIDi1450824.
KEGGimja:MJECL42.

Phylogenomic databases

eggNOGiCOG0286.
HOGENOMiHOG000239473.
InParanoidiQ60297.
KOiK03427.
OMAiLAENIFW.
PhylomeDBiQ60297.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiT1MH_METJA
AccessioniPrimary (citable) accession number: Q60297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.