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Q60295

- T1RH_METJA

UniProt

Q60295 - T1RH_METJA

Protein

Putative type-1 restriction enzyme MjaXP R protein

Gene

MJECL40

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. helicase activity Source: InterPro
    4. oxidoreductase activity Source: InterPro
    5. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Protein family/group databases

    REBASEi3908. MjaORFCL42P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative type-1 restriction enzyme MjaXP R protein (EC:3.1.21.3)
    Alternative name(s):
    Putative type I restriction enzyme MjaXP R protein
    Gene namesi
    Ordered Locus Names:MJECL40
    Encoded oniPlasmid large ECE0 Publication
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Plasmid large ECE

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10421042Putative type-1 restriction enzyme MjaXP R proteinPRO_0000077273Add
    BLAST

    Proteomic databases

    PRIDEiQ60295.

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

    Protein-protein interaction databases

    STRINGi243232.MJECL40.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini323 – 487165Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini551 – 731181Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HsdR family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0610.
    HOGENOMiHOG000240457.
    KOiK01153.
    OMAiIRYFVLF.
    PhylomeDBiQ60295.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR021810. DUF3387.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR011254. Prismane-like.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    [Graphical view]
    PfamiPF11867. DUF3387. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    SSF56821. SSF56821. 1 hit.
    TIGRFAMsiTIGR00348. hsdR. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60295-1 [UniParc]FASTAAdd to Basket

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    MPISANKYFL LVGVFIGDKM KKEAAKLNED YVVENAAIQR LKNLGYSYKH     50
    GSELTPEYNE RESYRDAILK NRFIKAIKNI NPWLTEELAL KVYKTVTNID 100
    NPDFNMRGKI FYEMLINGVK LEFKENGEKK TRFVKLIDFE NINKNEFLVA 150
    NQFEVEYYYE NGRFRRPDLV VFINGIPIAI FEFKSPKSNQ TAKDAFNDHK 200
    TKMKDIPQLY QYAQILVVSD GLETKYGSPT SDWDRFFVWE GVESDDDVEV 250
    IEVDNYGNTM YKYKGNPYTS LDILLMGLFK KEHLIEFLED FIIHDKKKII 300
    ATYYQFYTVK KAVDRTIKSV LYGETPEDRR IGIVWHAQGT GKSITMLFYA 350
    KKALKQKELN YPLLVFLTDR LELDEQLYNV FSSVFSEAER AESIAELQEL 400
    IKKTPGGIIF ATIQKFGRKS KDEHYPFLTD RNNIIIIADE AHRSHYGTLA 450
    QNLRKAIPNA SFLAFTATPI DYKDRSTFLV FGDYISAYPI DKAKRHGVVV 500
    PIYYEARLVE LHLTNEFIDL EFDEISERVA NDPETKESIK EVFAKLEKIM 550
    LTEDYLSKVS KDIIEHFNKR LQDFDGKAMV VTISRKVAVE LYKWITKQPN 600
    APKIAVVMSG NKSKDPEDFH PHIRTKKELE NLAKEFKDPE SDLKMVIVVD 650
    MWLTGFDVPC LHTMYFLKPM KNHSLAQAIA RVNRVFKDKP GGLIVDYIGI 700
    ADDLSKSLSK YSSEARKDLM TDIKVVIEEM KRRYEKVTSY FKNINYKDWK 750
    KLSSEDLSLL TVKAYQRVAK DDNTKKEFVR NVIALKKLYL LARPHPETIG 800
    IKDDLEFFEM IKKMIVKYST KKIREISQDL ENDIQSLISK SISAKELVDV 850
    FEMLKKEKPE LSVLSDEFLS EIAKIEYKDY VRDVLIKILN DDIRVRMAKN 900
    PIRFKKFSER LNEVIEKYRI KVITTAEMIE ELVNLAKEIR KAAEEGKELG 950
    LTEEELAFYD LLLSYPNIPL TDKKRVEKIA KEIARMMSGY IKARDWKKKK 1000
    NLQSKIRAKL KIILMKEGIK DYSLINKISD DLFEYAKNIY AI 1042
    Length:1,042
    Mass (Da):121,523
    Last modified:November 1, 1996 - v1
    Checksum:i3C8E5A103E7B59D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77118 Genomic DNA. Translation: AAC37109.1.
    PIRiG64514.
    RefSeqiNP_044166.1. NC_001732.1.

    Genome annotation databases

    EnsemblBacteriaiAAC37109; AAC37109; MJ_ECL40.
    GeneIDi1450822.
    KEGGimja:MJECL40.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77118 Genomic DNA. Translation: AAC37109.1 .
    PIRi G64514.
    RefSeqi NP_044166.1. NC_001732.1.

    3D structure databases

    ProteinModelPortali Q60295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJECL40.

    Protein family/group databases

    REBASEi 3908. MjaORFCL42P.

    Proteomic databases

    PRIDEi Q60295.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC37109 ; AAC37109 ; MJ_ECL40 .
    GeneIDi 1450822.
    KEGGi mja:MJECL40.

    Phylogenomic databases

    eggNOGi COG0610.
    HOGENOMi HOG000240457.
    KOi K01153.
    OMAi IRYFVLF.
    PhylomeDBi Q60295.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR021810. DUF3387.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR011254. Prismane-like.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    [Graphical view ]
    Pfami PF11867. DUF3387. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    SSF56821. SSF56821. 1 hit.
    TIGRFAMsi TIGR00348. hsdR. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiT1RH_METJA
    AccessioniPrimary (citable) accession number: Q60295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3