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Q60295

- T1RH_METJA

UniProt

Q60295 - T1RH_METJA

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Protein

Putative type-1 restriction enzyme MjaXP R protein

Gene

MJECL40

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. oxidoreductase activity Source: InterPro
  4. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

REBASEi3908. MjaORFCL42P.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type-1 restriction enzyme MjaXP R protein (EC:3.1.21.3)
Alternative name(s):
Putative type I restriction enzyme MjaXP R protein
Gene namesi
Ordered Locus Names:MJECL40
Encoded oniPlasmid large ECE0 Publication
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Plasmid large ECE

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Putative type-1 restriction enzyme MjaXP R proteinPRO_0000077273Add
BLAST

Proteomic databases

PRIDEiQ60295.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

Protein-protein interaction databases

STRINGi243232.MJECL40.

Structurei

3D structure databases

ProteinModelPortaliQ60295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini323 – 487165Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini551 – 731181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HsdR family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0610.
HOGENOMiHOG000240457.
InParanoidiQ60295.
KOiK01153.
OMAiIRYFVLF.
PhylomeDBiQ60295.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR021810. DUF3387.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR011254. Prismane-like.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
[Graphical view]
PfamiPF11867. DUF3387. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF56821. SSF56821. 1 hit.
TIGRFAMsiTIGR00348. hsdR. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60295-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPISANKYFL LVGVFIGDKM KKEAAKLNED YVVENAAIQR LKNLGYSYKH
60 70 80 90 100
GSELTPEYNE RESYRDAILK NRFIKAIKNI NPWLTEELAL KVYKTVTNID
110 120 130 140 150
NPDFNMRGKI FYEMLINGVK LEFKENGEKK TRFVKLIDFE NINKNEFLVA
160 170 180 190 200
NQFEVEYYYE NGRFRRPDLV VFINGIPIAI FEFKSPKSNQ TAKDAFNDHK
210 220 230 240 250
TKMKDIPQLY QYAQILVVSD GLETKYGSPT SDWDRFFVWE GVESDDDVEV
260 270 280 290 300
IEVDNYGNTM YKYKGNPYTS LDILLMGLFK KEHLIEFLED FIIHDKKKII
310 320 330 340 350
ATYYQFYTVK KAVDRTIKSV LYGETPEDRR IGIVWHAQGT GKSITMLFYA
360 370 380 390 400
KKALKQKELN YPLLVFLTDR LELDEQLYNV FSSVFSEAER AESIAELQEL
410 420 430 440 450
IKKTPGGIIF ATIQKFGRKS KDEHYPFLTD RNNIIIIADE AHRSHYGTLA
460 470 480 490 500
QNLRKAIPNA SFLAFTATPI DYKDRSTFLV FGDYISAYPI DKAKRHGVVV
510 520 530 540 550
PIYYEARLVE LHLTNEFIDL EFDEISERVA NDPETKESIK EVFAKLEKIM
560 570 580 590 600
LTEDYLSKVS KDIIEHFNKR LQDFDGKAMV VTISRKVAVE LYKWITKQPN
610 620 630 640 650
APKIAVVMSG NKSKDPEDFH PHIRTKKELE NLAKEFKDPE SDLKMVIVVD
660 670 680 690 700
MWLTGFDVPC LHTMYFLKPM KNHSLAQAIA RVNRVFKDKP GGLIVDYIGI
710 720 730 740 750
ADDLSKSLSK YSSEARKDLM TDIKVVIEEM KRRYEKVTSY FKNINYKDWK
760 770 780 790 800
KLSSEDLSLL TVKAYQRVAK DDNTKKEFVR NVIALKKLYL LARPHPETIG
810 820 830 840 850
IKDDLEFFEM IKKMIVKYST KKIREISQDL ENDIQSLISK SISAKELVDV
860 870 880 890 900
FEMLKKEKPE LSVLSDEFLS EIAKIEYKDY VRDVLIKILN DDIRVRMAKN
910 920 930 940 950
PIRFKKFSER LNEVIEKYRI KVITTAEMIE ELVNLAKEIR KAAEEGKELG
960 970 980 990 1000
LTEEELAFYD LLLSYPNIPL TDKKRVEKIA KEIARMMSGY IKARDWKKKK
1010 1020 1030 1040
NLQSKIRAKL KIILMKEGIK DYSLINKISD DLFEYAKNIY AI
Length:1,042
Mass (Da):121,523
Last modified:November 1, 1996 - v1
Checksum:i3C8E5A103E7B59D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77118 Genomic DNA. Translation: AAC37109.1.
PIRiG64514.
RefSeqiNP_044166.1. NC_001732.1.
WP_010890087.1. NC_001732.1.

Genome annotation databases

EnsemblBacteriaiAAC37109; AAC37109; MJ_ECL40.
GeneIDi1450822.
KEGGimja:MJECL40.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77118 Genomic DNA. Translation: AAC37109.1 .
PIRi G64514.
RefSeqi NP_044166.1. NC_001732.1.
WP_010890087.1. NC_001732.1.

3D structure databases

ProteinModelPortali Q60295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJECL40.

Protein family/group databases

REBASEi 3908. MjaORFCL42P.

Proteomic databases

PRIDEi Q60295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC37109 ; AAC37109 ; MJ_ECL40 .
GeneIDi 1450822.
KEGGi mja:MJECL40.

Phylogenomic databases

eggNOGi COG0610.
HOGENOMi HOG000240457.
InParanoidi Q60295.
KOi K01153.
OMAi IRYFVLF.
PhylomeDBi Q60295.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR021810. DUF3387.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR011254. Prismane-like.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
[Graphical view ]
Pfami PF11867. DUF3387. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
SSF56821. SSF56821. 1 hit.
TIGRFAMsi TIGR00348. hsdR. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiT1RH_METJA
AccessioniPrimary (citable) accession number: Q60295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3