Q601V4 (Q601V4_MYCH2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL AAV27412.1
Ordered Locus Names:	mhp097

Organism

Mycoplasma hyopneumoniae (strain 232) [Complete proteome] [HAMAP]

Taxonomic identifier

295358 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q601V4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: BDD5E9C96C4BF2EA	FASTA	636	73,256
	10 20 30 40 50 60 MEKLIIVESP NKINTISSYL DSSYKVTACV GHITNLAKTG QFGLGIDINT WTPSYVLDKT 70 80 90 100 110 120 KKKTIDEIKK LAKQAKTVII ATDADREGEA IGASLVEHLA ITDKYQRIKY NEITKTAILE 130 140 150 160 170 180 ALENPGELNQ NLINSQQTRR MLDRIIGFRL TALLKNKIKN PPKNPAAGRV QSFALSLIFD 190 200 210 220 230 240 REKEIREFIP EKYFNLEVKI NDENGNFFDT IYCNQKNEAK KVNWINSEEE LSEIIAEIKE 250 260 270 280 290 300 NPYLIFKKYS KATRKDPKVI PLKQATIFKK MSQYSSASVS NALQKLYEGY GDYGLISYPR 310 320 330 340 350 360 TDSTRLSQTF IEKGKEFIIK NFGPKYFTKS IKGFAGDQDA HEAIRPTNLE LSPELAREKY 370 380 390 400 410 420 HLGQIEFQIY QLIYQTTIQA LMRSPERKIT NLTFKTPIKD HIFSYSYSEF TFDGYYKATE 430 440 450 460 470 480 KPSLKFKPEF SDIESLISKK FQVDKNFDNS IKSSLHQTKP PAHFRDGTLI EKLDDEKVGR 490 500 510 520 530 540 PSTFAISVKK LKDHLYVDSE SKSLVLSDFG KIVYENLKII SPEIINAKFT AQVEEELDLI 550 560 570 580 590 600 AQGKQDYKKY LDSMWSKIEN TIEKAVEVEK VIMEARTTGE KCTECDADLV YRYNRKTNQR 610 620 630 FIGCSSFPNC RFIKVDENAK KVYRFRKRNT KILTNK

References

[1]

"The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis." Minion F.C., Lefkowitz E.J., Madsen M.L., Cleary B.J., Swartzell S.M., Mahairas G.G. J. Bacteriol. 186:7123-7133(2004) [PubMed: 15489423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017332 Genomic DNA. Translation: AAV27412.1.
RefSeq	YP_115611.1. NC_006360.1.
3D structure databases
ProteinModelPortal	Q601V4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q601V4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3105411.
GenomeReviews	Gene locus mhp097 in contig AE017332_GR.
KEGG	mhy:mhp097.
PATRIC	20010737. VBIMycHyo77580_0099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	DKLVGYK.
PhylomeDB	Q601V4.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q601V4_MYCH2

Accession

Primary (citable) accession number: Q601V4

Entry history

Integrated into UniProtKB/TrEMBL:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)