Q601G1 (SYE_MYCH2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	mhp241

Organism

Mycoplasma hyopneumoniae (strain 232) [Complete proteome] [HAMAP]

Taxonomic identifier

295358 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119602
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	250 – 254	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	253	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q601G1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 99DEE8A445131E1B	FASTA	467	55,282
	10 20 30 40 50 60 MKIRTRYAPS PTGFLHIGGA RTALFNYLFA KHHNGDFILR IEDSDNSRNI KDGEKSQIEN 70 80 90 100 110 120 LLWLGIIPDE KPGSETKFGP YRQSEKLERY QKLAQELIKK GFAYYAFDNQ EELELQKKEQ 130 140 150 160 170 180 IAKGIFSFRY DQNWLKISDQ EKQKRLKNKH FVIRFKVDKA KNFCWNDLVR GQICFEGSAI 190 200 210 220 230 240 SDWVIIKSDG FPTYNFAVVV DDFDMEISHI FRGEEHISNT PKQIGIYQAF NWKTPKFGHL 250 260 270 280 290 300 TIITDKNGKK LSKRDKSLFQ FIEDYKNQGY HSEAFFNFLA LLGWTSPDSQ EFFDHKSLIK 310 320 330 340 350 360 AFDYKRLSKA PSYFDIEKLN WFSKSYISKM PVDKILENLE LSDNQIWNQF FVETFQKSSI 370 380 390 400 410 420 KYADFYKNFE FFHRPKQEMD EKMLEIFEKL DKKPVKIFAS KIDYQNWDYT KINDLIKEIG 430 440 450 460 QKLEITGKNL LLPIRLATTF TNSGPELARA IWLLGKKIIE KRLLKWK

References

[1]

"The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis." Minion F.C., Lefkowitz E.J., Madsen M.L., Cleary B.J., Swartzell S.M., Mahairas G.G. J. Bacteriol. 186:7123-7133(2004) [PubMed: 15489423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 232.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017332 Genomic DNA. Translation: AAV27782.1.
RefSeq	YP_115754.1. NC_006360.1.
3D structure databases
ProteinModelPortal	Q601G1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q601G1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3105790.
GenomeReviews	Gene locus mhp241 in contig AE017332_GR.
KEGG	mhy:mhp241.
PATRIC	20011055. VBIMycHyo77580_0252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DDFDMEI.
PhylomeDB	Q601G1.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MHYO295358:MHP241-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCH2

Accession

Primary (citable) accession number: Q601G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program