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Protein

Glutamine synthetase

Gene

glnA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Beta-glutamate is a much poorer substrate than alpha-glutamate.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Feedback inhibited by glycine and alanine, and inhibited by low concentrations of methionine sulfoximine.1 Publication

Kineticsi

  1. KM=23 mM for alpha-glutamine (at 37 degrees Celsius)1 Publication
  2. KM=40 mM for alpha-glutamine (at 50 degrees Celsius)1 Publication
  3. KM=58 mM for alpha-glutamate (at 60 degrees Celsius)1 Publication
  1. Vmax=26.7 µmol/min/mg enzyme toward alpha-glutamine (at 50 degrees Celsius)1 Publication
  2. Vmax=3.5 µmol/min/mg enzyme toward alpha-glutamine (at 37 degrees Celsius)1 Publication
  3. Vmax=1.3 µmol/min/mg enzyme toward alpha-glutamate (at 60 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Magnesium 1By similarity1
Metal bindingi144Magnesium 2By similarity1
Binding sitei194ATPBy similarity1
Metal bindingi199Magnesium 2By similarity1
Metal bindingi206Magnesium 2By similarity1
Binding sitei251L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi255Magnesium 1; via pros nitrogenBy similarity1
Binding sitei259ATPBy similarity1
Binding sitei309L-glutamateBy similarity1
Binding sitei315L-glutamateBy similarity1
Binding sitei327ATPBy similarity1
Binding sitei327L-glutamateBy similarity1
Binding sitei332ATPBy similarity1
Binding sitei339ATPBy similarity1
Metal bindingi344Magnesium 1By similarity1
Binding sitei346L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 259ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.21 Publication)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alpha1 Publication
Short name:
GSI alpha1 Publication
Gene namesi
Name:glnABy similarity
Ordered Locus Names:MJ1346
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532041 – 454Glutamine synthetaseAdd BLAST454

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1346.

Structurei

3D structure databases

ProteinModelPortaliQ60182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni250 – 251L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
InParanoidiQ60182.
KOiK01915.
OMAiTFYTHPE.
OrthoDBiPOG093Z00GE.
PhylomeDBiQ60182.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWGMEMNVE QAIEYVKKNN VKFIRFQFVD ILGFPKNVAY PVKAGEKGIE
60 70 80 90 100
ELREIFENGV WFDGSSITGF VGIEESDMLL KPDLSTLSVL PWRPEEKSVA
110 120 130 140 150
RVICDVYKDE KTPFEGDPRS RLKAILEELK KEMNGEYFVG PEPEFFLLKR
160 170 180 190 200
DPHNPHRWVP ADDGGYFDVE PLDDAPDIRR DIVLALENLG FHVEASHHEV
210 220 230 240 250
APGQHEVDFK FDNALKTADS VITFKMTIKN IAKKHGLKAT FMPKPFFGMN
260 270 280 290 300
GNGMHCHQSV WFNGEPSFYD PEGPYNGLSE TCLSYIAGIL SHAKALVAIT
310 320 330 340 350
NPTVNSYKRL VPGYEAPVNI AWANKNRSAI IRVPAARGKA TRIEFRAPDP
360 370 380 390 400
TCNPYLAFAC MLAAGLDGIK KKMTAPEPVE RNIFKMSEEE KKQLGIESVP
410 420 430 440 450
ANLAAALDEL ECDEVLQKAL GKHIYENYME IKRAEWDDFR TAVTDWETGK

YLIY
Length:454
Mass (Da):51,399
Last modified:November 1, 1997 - v1
Checksum:i3222CBA464D18120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99355.1.
PIRiA64468.

Genome annotation databases

EnsemblBacteriaiAAB99355; AAB99355; MJ_1346.
KEGGimja:MJ_1346.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_METJA
AccessioniPrimary (citable) accession number: Q60182
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families