Q60181 (RPOB1_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit B'
EC=2.7.7.6

Gene names

Name:	rpoB1
Ordered Locus Names:	MJ1041

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	DNA-directed RNA polymerase subunit B'		PRO_0000048102
Regions
	Zinc finger	551 – 572	22	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60181 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 81DE30267A572914	FASTA	636	72,193
	10 20 30 40 50 60 MKNLASREVN IYVNGKLVGT TDKPEELVNF IREKRRKGEL PQYTTVAYNE ESNDIHINTD 70 80 90 100 110 120 AGRIVRPLIV VENGKPKLTK EHIEKLKKGE ITFSDLVKEG VIEYLDAEEE ENAYIALSEE 130 140 150 160 170 180 ELTEKHTHLE IDPLTILGIG AGVAPYPEHN SAPRITMAAA MGKQSLGIPM SNIKWRLDTR 190 200 210 220 230 240 GHYLHYPQVP IVRTKHQEIL GFDKRPAGQN FVVAIMSYEG YNMEDAIVFN KSAIDRGLGR 250 260 270 280 290 300 STFFRTYDAC ERRYPGGQMD RFEIPDKGVR GYRSEECYRY LEEDGIVAVE SHVKGGDVIV 310 320 330 340 350 360 GKTSPPRFLE EHEITIQVKP QRRDSSVVVR HGEEGYIDKV ILTETKEGNR LVKVKVRDLR 370 380 390 400 410 420 IPELGDKFAS RHGQKGVMGL TVPQEDLPFT ESGIVPDIII NPHAIPSRMT VGQLLEMLGG 430 440 450 460 470 480 KVGALEGRRI DGTIFSGEKE WDLRKALEAL GFKHHGKEVM YDGKTGKKFE VEIYIGIAYY 490 500 510 520 530 540 QKLHHLVAGK IHARSRGPVQ VLTRQPTEGR AREGGLRFGE MERDVLIGHG AAMLLKERLM 550 560 570 580 590 600 DESDPYDICI CSKCGDFAIL DYKRGLKYCP ICGEIENLYS SKKIPFVRIP YAFKLLLDEL 610 620 630 KSMCILPRIK VRDKVELEDF EEFVEKLEKE KVKQKQ

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99044.1.
PIR	H64429.
RefSeq	NP_248035.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60181.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451938.
GenomeReviews	Gene locus MJ1041 in contig L77117_GR.
KEGG	mja:MJ_1041.
NMPDR	fig|243232.1.peg.1072.
TIGR	MJ1041.
Phylogenomic databases
HOGENOM	HBG496836.
OMA	MSYEGYN.
ProtClustDB	PRK07225.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1041-MONOMER.
Family and domain databases
InterPro	IPR007120. DNA-dir_RNA_pol_su2_6. IPR019969. DNA-dir_RNA_pol_su_B_arc. IPR007121. RNA_pol_bsu_CS. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KO	K03044.
Pfam	PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR03670. RpoB_arch. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB1_METJA

Accession

Primary (citable) accession number: Q60181

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program