Q60180 (TRPA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	MJ1038

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. HAMAP MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.
Sequence caution	The sequence AAB99041.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Tryptophan synthase alpha chain HAMAP MF_00131		PRO_0000098889
Sites
	Active site	49	1	Proton acceptor By similarity
	Active site	60	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60180 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: D68E352004FB1E9F	FASTA	281	31,271
	10 20 30 40 50 60 MIMKLAEKFE ELKNKGEKAF VAFYVGGDPN LEISEKALEV ICKHADIVEI GIPFSDPVAD 70 80 90 100 110 120 GITIQKADVR ALNSGMNPLK AFELAKKLNE KAPNVPKVFL TYYNIIFKMG EEEFVKKCKE 130 140 150 160 170 180 AGVSGIIVPD LPIEEADSLY NYCKKYGVDL IFLVAPTTPD ERLKKILEKC SGFVYVVSVT 190 200 210 220 230 240 GITGAREKVA EETKELIKRV KKFSKIPACV GFGISKREHV EEITEIADGA IVGSAIVKIV 250 260 270 280 EKHLDENGQI KDEEKFLKEL EEFVKNLKEG TKKKAKVAIK N

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99041.1. Different initiation.
PIR	E64429.
RefSeq	NP_248032.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60180.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451935.
GenomeReviews	Gene locus MJ1038 in contig L77117_GR.
KEGG	mja:MJ_1038.
NMPDR	fig|243232.1.peg.1069.
TIGR	MJ1038.
Phylogenomic databases
HOGENOM	HBG391690.
OMA	VTGTDTK.
ProtClustDB	CLSK2517682.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1038-MONOMER.
Family and domain databases
HAMAP	MF_00131. Trp_synth_alpha. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01695.
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. TrpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_METJA

Accession

Primary (citable) accession number: Q60180

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program