Q60179 (TRPB_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase beta chain
EC=4.2.1.20

Gene names

Name:	trpB
Ordered Locus Names:	MJ1037

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine. HAMAP MF_00133
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00133
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00133
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00133
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Tryptophan synthase beta chain HAMAP MF_00133		PRO_0000099038
Amino acid modifications
	Modified residue	98	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60179 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A75D876F95EA8529	FASTA	404	44,364
	10 20 30 40 50 60 MSILKKYKDM YPDENGKFGI YGGKFVPETL MPAIAELEEA FKRFWINNEG NFREEFYALL 70 80 90 100 110 120 RDYVGRPTPL YYAERLSEEL GCKVYLKRED LAHLGAHKIN NALGQALLAK KMGKKRVIAE 130 140 150 160 170 180 TGAGQHGVAT AAACAKLGLE CIIYMGAKDV ERQKLNVFRM ELMGAKVIPV FGGSQTLKDA 190 200 210 220 230 240 VNEALRDWTT NVRTTYYLLG SALGPHPYPM MVREFQRVIG KELKEQILEK EGRLPDVIVA 250 260 270 280 290 300 CVGGGSNAIG AFYEFLDDDV ELYAVEAGGK GIETGMHGAS LCAGEVGVLH GAKIYVKEDE 310 320 330 340 350 360 FGQIEESYSI SAGLDYPGVG PELSFLKDEG RIKAVCVTDD EALEAFQLLC RLEGILPALE 370 380 390 400 SSHALAYAVK LADKLDKDDI MVINLSGRGD KDVQTVAKAL GREI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99040.1.
PIR	D64429.
RefSeq	NP_248031.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60179.
SMR	Q60179. Positions 18-401.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451934.
GenomeReviews	Gene locus MJ1037 in contig L77117_GR.
KEGG	mja:MJ_1037.
NMPDR	fig|243232.1.peg.1068.
TIGR	MJ1037.
Phylogenomic databases
HOGENOM	HBG303148.
OMA	IMVINLS.
ProtClustDB	PRK04346.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1037-MONOMER.
Family and domain databases
HAMAP	MF_00133. Trp_synth_beta. [Tree]
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR006653. Trp_synth_b_CS. IPR006654. Trp_synth_beta. IPR023026. Trp_synth_beta/beta-like. [Graphical view]
KO	K01696.
PANTHER	PTHR10314:SF3. PTHR10314:SF3. 1 hit.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF001413. Trp_syn_beta. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00263. TrpB. 1 hit.
PROSITE	PS00168. TRP_SYNTHASE_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPB_METJA

Accession

Primary (citable) accession number: Q60179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program