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Q60178

- HEM2_METJA

UniProt

Q60178 - HEM2_METJA

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi129 – 1291Zinc; catalyticBy similarity
    Metal bindingi131 – 1311Zinc; catalyticBy similarity
    Metal bindingi139 – 1391Zinc; catalyticBy similarity
    Active sitei204 – 2041Schiff-base intermediate with substrateBy similarity
    Binding sitei214 – 2141Substrate 1By similarity
    Binding sitei226 – 2261Substrate 1By similarity
    Metal bindingi242 – 2421MagnesiumBy similarity
    Active sitei257 – 2571Schiff-base intermediate with substrateBy similarity
    Binding sitei283 – 2831Substrate 2By similarity
    Binding sitei322 – 3221Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:MJ0643
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Delta-aminolevulinic acid dehydratasePRO_0000140523Add
    BLAST

    Proteomic databases

    PRIDEiQ60178.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi243232.MJ0643.

    Structurei

    3D structure databases

    ProteinModelPortaliQ60178.
    SMRiQ60178. Positions 11-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    KOiK01698.
    OMAiDMILTYF.
    PhylomeDBiQ60178.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKINLGDFM LIRPRRLRKN QKIRDLVRET ILTKNDLIMP IFVDENLKGN    50
    EKKEISSMPN QYRFSVEGAI EEAKEIADLG IPAVILFGIP KHKDEIASSA 100
    YDKNGVVQRT IRGIKEELGD ELLVIADCCL CEYTSHGHCG IVKDGKILND 150
    ATLPILAKIA LSYADAGVDI VAPSDMMDGR VRAIREILEE NGYDDVAIMS 200
    YSAKYASSFY GPFREAAESA PKFGDRKSYQ MDIGNAREAL KEIALDIEEG 250
    ADLILVKPAL PYLDIIRMAK DRFDVPIGGY CVSGEYAMVE AAARNGWLDR 300
    EKVIYEILLS IKRAGADFII TYWAKEVAEI GLSQE 335
    Length:335
    Mass (Da):37,396
    Last modified:November 1, 1997 - v1
    Checksum:i4D097DAB45F3705D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98637.1.
    PIRiC64380.
    RefSeqiNP_247627.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98637; AAB98637; MJ_0643.
    GeneIDi1451509.
    KEGGimja:MJ_0643.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98637.1 .
    PIRi C64380.
    RefSeqi NP_247627.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q60178.
    SMRi Q60178. Positions 11-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0643.

    Proteomic databases

    PRIDEi Q60178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98637 ; AAB98637 ; MJ_0643 .
    GeneIDi 1451509.
    KEGGi mja:MJ_0643.

    Phylogenomic databases

    eggNOGi COG0113.
    KOi K01698.
    OMAi DMILTYF.
    PhylomeDBi Q60178.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiHEM2_METJA
    AccessioniPrimary (citable) accession number: Q60178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3