Reviewed, UniProtKB/Swiss-Prot Q60178 (HEM2_METJA)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	MJ0643

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Delta-aminolevulinic acid dehydratase		PRO_0000140523
Regions
	Region	126 – 144	19	Zinc-binding By similarity
Sites
	Active site	257	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60178-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4D097DAB45F3705D	FASTA	335	37,396
	10 20 30 40 50 60 MFKINLGDFM LIRPRRLRKN QKIRDLVRET ILTKNDLIMP IFVDENLKGN EKKEISSMPN 70 80 90 100 110 120 QYRFSVEGAI EEAKEIADLG IPAVILFGIP KHKDEIASSA YDKNGVVQRT IRGIKEELGD 130 140 150 160 170 180 ELLVIADCCL CEYTSHGHCG IVKDGKILND ATLPILAKIA LSYADAGVDI VAPSDMMDGR 190 200 210 220 230 240 VRAIREILEE NGYDDVAIMS YSAKYASSFY GPFREAAESA PKFGDRKSYQ MDIGNAREAL 250 260 270 280 290 300 KEIALDIEEG ADLILVKPAL PYLDIIRMAK DRFDVPIGGY CVSGEYAMVE AAARNGWLDR 310 320 330 EKVIYEILLS IKRAGADFII TYWAKEVAEI GLSQE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98637.1.
PIR	C64380.
RefSeq	NP_247627.1.
3D structure databases
HSSP	HSSP built from PDB template 1E51 based on UniProtKB P13716.
ModBase	Search...
Genome annotation databases
GeneID	1451509.
GenomeReviews	Gene locus MJ0643 in contig L77117_GR.
KEGG	mja:MJ0643.
NMPDR	fig|243232.1.peg.664.
TIGR	MJ0643.
Phylogenomic databases
HOGENOM	Q60178.
OMA	PELGVIT.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0643-MON.
BRENDA	4.2.1.24. 256362.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_METJA

Accession

Primary (citable) accession number: Q60178

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)