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Protein

Proteasome subunit alpha

Gene

psmA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.jannaschii proteasome is able to cleave oligopeptides after Glu, Asp, Tyr, Phe, Trp, slightly after Arg, but not after Ala. Thus, displays caspase-like and chymotrypsin-like activities and low level of trypsin-like activity.UniRule annotation1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation1 Publication

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

GO - Molecular functioni

  1. endopeptidase activity Source: UniProtKB
  2. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome alpha subunitUniRule annotation
Proteasome core protein PsmAUniRule annotation
Gene namesi
Name:psmAUniRule annotation
Ordered Locus Names:MJ0591
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, alpha-subunit complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Proteasome subunit alphaPRO_0000124174Add
BLAST

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi243232.MJ0591.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 3311
Beta strandi48 – 525
Turni62 – 643
Beta strandi68 – 725
Beta strandi75 – 806
Helixi84 – 907
Helixi93 – 10210
Helixi110 – 12314
Beta strandi136 – 1405
Beta strandi149 – 1524
Beta strandi159 – 1635
Turni171 – 1766
Helixi177 – 1804
Helixi192 – 20110
Beta strandi202 – 2043
Beta strandi209 – 2168
Turni217 – 2204
Beta strandi221 – 2244
Turni227 – 2304
Helixi231 – 2333
Turni234 – 2396
Beta strandi240 – 2423

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H4PX-ray4.10A/B/C/D/E/F/G/H/I/J/K/L/M/N1-261[»]
ProteinModelPortaliQ60177.
SMRiQ60177. Positions 5-247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60177.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ60177.
KOiK03432.
OMAiEKNYKYD.
PhylomeDBiQ60177.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQMVPPSAYD RAITVFSPEG RLYQVEYARE AVRRGTTAIG IACKDGVVLA
60 70 80 90 100
VDRRITSKLV KIRSIEKIFQ IDDHVAAATS GLVADARVLI DRARLEAQIY
110 120 130 140 150
RLTYGEEISI EMLAKKICDI KQAYTQHGGV RPFGVSLLIA GIDKNEARLF
160 170 180 190 200
ETDPSGALIE YKATAIGSGR PVVMELLEKE YRDDITLDEG LELAITALTK
210 220 230 240 250
ANEDIKPENV DVCIITVKDA QFKKIPVEEI KKLIEKVKKK LNEENKKEEE
260
NREETKEKQE E
Length:261
Mass (Da):29,321
Last modified:November 1, 1997 - v1
Checksum:i93FC7DF277E01AD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98581.1.
PIRiG64373.
RefSeqiNP_247571.1. NC_000909.1.
WP_010870095.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98581; AAB98581; MJ_0591.
GeneIDi1451456.
KEGGimja:MJ_0591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98581.1.
PIRiG64373.
RefSeqiNP_247571.1. NC_000909.1.
WP_010870095.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H4PX-ray4.10A/B/C/D/E/F/G/H/I/J/K/L/M/N1-261[»]
ProteinModelPortaliQ60177.
SMRiQ60177. Positions 5-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ0591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98581; AAB98581; MJ_0591.
GeneIDi1451456.
KEGGimja:MJ_0591.

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ60177.
KOiK03432.
OMAiEKNYKYD.
PhylomeDBiQ60177.

Miscellaneous databases

EvolutionaryTraceiQ60177.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome."
    Wilson H.L., Ou M.S., Aldrich H.C., Maupin-Furlow J.
    J. Bacteriol. 182:1680-1692(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, INTERACTION WITH PAN.
  3. "Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii."
    Zhang F., Hu M., Tian G., Zhang P., Finley D., Jeffrey P.D., Shi Y.
    Mol. Cell 34:473-484(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT, GATED STRUCTURE, SUBUNIT.

Entry informationi

Entry nameiPSA_METJA
AccessioniPrimary (citable) accession number: Q60177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.