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Q60176

- MDH_METJA

UniProt

Q60176 - MDH_METJA

Protein

Malate dehydrogenase

Gene

mdh

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate. Also catalyzes the reduction of sulfopyruvate, but not pyruvate, nor alpha-ketoglutarate. Higher selectivity for the coenzyme NADPH.2 Publications

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.
    (S)-malate + NADP+ = oxaloacetate + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861SubstrateBy similarity
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei99 – 991NADP1 Publication
    Binding sitei123 – 1231SubstrateBy similarity
    Binding sitei154 – 1541SubstrateBy similarity
    Active sitei178 – 1781Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADP1 Publication
    Nucleotide bindingi34 – 374NADP1 Publication
    Nucleotide bindingi121 – 1233NADP1 Publication

    GO - Molecular functioni

    1. L-lactate dehydrogenase activity Source: InterPro
    2. L-malate dehydrogenase activity Source: UniProtKB-HAMAP
    3. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.272. 3263.
    SABIO-RKQ60176.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37, EC:1.1.1.82)
    Gene namesi
    Name:mdh
    Synonyms:mdhB
    Ordered Locus Names:MJ0490
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Malate dehydrogenasePRO_0000113485Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    STRINGi243232.MJ0490.

    Structurei

    Secondary structure

    1
    313
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Turni7 – 93
    Helixi11 – 2111
    Beta strandi28 – 336
    Helixi35 – 373
    Helixi38 – 5215
    Beta strandi60 – 656
    Helixi69 – 724
    Beta strandi76 – 805
    Helixi92 – 11322
    Beta strandi117 – 1204
    Beta strandi122 – 1243
    Helixi125 – 13612
    Beta strandi142 – 1454
    Helixi149 – 16315
    Helixi167 – 1693
    Beta strandi174 – 1763
    Beta strandi182 – 1843
    Helixi186 – 1883
    Helixi196 – 1983
    Helixi200 – 2045
    Helixi207 – 21711
    Helixi219 – 2224
    Beta strandi225 – 2273
    Helixi232 – 24413
    Beta strandi249 – 26315
    Beta strandi265 – 27612
    Beta strandi279 – 2835
    Helixi290 – 31021

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HYEX-ray1.90A1-313[»]
    1HYGX-ray2.80A/B1-313[»]
    ProteinModelPortaliQ60176.
    SMRiQ60176. Positions 1-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60176.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    KOiK00024.
    OMAiDSDKDQW.
    PhylomeDBiQ60176.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q60176-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVTIIGASG RVGSATALLL AKEPFMKDLV LIGREHSINK LEGLREDIYD    50
    ALAGTRSDAN IYVESDENLR IIDESDVVII TSGVPRKEGM SRMDLAKTNA 100
    KIVGKYAKKI AEICDTKIFV ITNPVDVMTY KALVDSKFER NQVFGLGTHL 150
    DSLRFKVAIA KFFGVHIDEV RTRIIGEHGD SMVPLLSATS IGGIPIQKFE 200
    RFKELPIDEI IEDVKTKGEQ IIRLKGGSEF GPAAAILNVV RCIVNNEKRL 250
    LTLSAYVDGE FDGIRDVCIG VPVKIGRDGI EEVVSIELDK DEIIAFRKSA 300
    EIIKKYCEEV KNL 313
    Length:313
    Mass (Da):34,609
    Last modified:November 1, 1997 - v1
    Checksum:iA7C0DB67C50151CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98481.1.
    PIRiB64361.
    RefSeqiNP_247466.1. NC_000909.1.
    WP_010869991.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98481; AAB98481; MJ_0490.
    GeneIDi1451352.
    KEGGimja:MJ_0490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98481.1 .
    PIRi B64361.
    RefSeqi NP_247466.1. NC_000909.1.
    WP_010869991.1. NC_000909.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HYE X-ray 1.90 A 1-313 [» ]
    1HYG X-ray 2.80 A/B 1-313 [» ]
    ProteinModelPortali Q60176.
    SMRi Q60176. Positions 1-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98481 ; AAB98481 ; MJ_0490 .
    GeneIDi 1451352.
    KEGGi mja:MJ_0490.

    Phylogenomic databases

    eggNOGi COG0039.
    KOi K00024.
    OMAi DSDKDQW.
    PhylomeDBi Q60176.

    Enzyme and pathway databases

    BRENDAi 1.1.1.272. 3263.
    SABIO-RK Q60176.

    Miscellaneous databases

    EvolutionaryTracei Q60176.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea."
      Graupner M., Xu H., White R.H.
      J. Bacteriol. 182:3688-3692(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "The putative L-lactate dehydrogenase from Methanococcus jannaschii is an NADPH-dependent L-malate dehydrogenase."
      Madern D.
      Mol. Microbiol. 37:1515-1520(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Differences in the oligomeric states of the LDH-like L-MalDH from the hyperthermophilic archaea Methanococcus jannaschii and Archaeoglobus fulgidus."
      Madern D., Ebel C., Dale H.A., Lien T., Steen I.H., Birkeland N.-K., Zaccai G.
      Biochemistry 40:10310-10316(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    5. "Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases."
      Lee B.I., Chang C., Cho S.-J., Eom S.H., Kim K.K., Yu Y.G., Suh S.W.
      J. Mol. Biol. 307:1351-1362(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.

    Entry informationi

    Entry nameiMDH_METJA
    AccessioniPrimary (citable) accession number: Q60176
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3