Q60174 (ASSY_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	MJ0429

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Argininosuccinate synthase HAMAP-Rule MF_00005		PRO_0000148676
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity
Sites
	Binding site	86	1	Citrulline By similarity
	Binding site	116	1	ATP; via amide nitrogen By similarity
	Binding site	118	1	Aspartate By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	122	1	Citrulline By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	173	1	Citrulline By similarity
	Binding site	182	1	Citrulline By similarity
	Binding site	257	1	Citrulline By similarity
	Binding site	269	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60174 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 655D4A7AC06E7A7F	FASTA	395	44,723
	10 20 30 40 50 60 MERIAVLAYS GGLDTSCCLK LLEDKYGYKV VSVCVDVGQP EEEIKEVEEK AKKLGVLKHY 70 80 90 100 110 120 TIDAKEEFVK DYIFRAIKAN AMYEGYPLST ALARPLIAHK VVEIAEEVGA EAVAHGCTGK 130 140 150 160 170 180 GNDQFRFETT IRIKAPHLKI IAPIRDLNLT RAEEIEYAKE KGIPIPTESK KYSIDENLWG 190 200 210 220 230 240 RSIEGSELEN PDFVPPEEIY AWTKNPVEDK EEEIVEIEFK EGVPVAINGE KLEPVELIKK 250 260 270 280 290 300 ANEIAGKHGV GRIDIIEDRI IGLKSRENYE CPGAVLLLTA HKALEQLVLT RDELRFKEIV 310 320 330 340 350 360 DSLYGELIYK GLWFDPLRED LDAFIDKTQE RVTGTVKVKL FGGTARVVGR DSPYALYSKE 370 380 390 LVSFDEKEID QKELAGMVKY HGLQAMLYEM RKKRK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98414.1.
PIR	E64353.
RefSeq	NP_247403.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q60174.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0429.
Proteomic databases
PRIDE	Q60174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98414; AAB98414; MJ_0429.
GeneID	1451289.
KEGG	mja:MJ_0429.
Phylogenomic databases
eggNOG	COG0137.
KO	K01940.
OMA	NDQFRFE.
ProtClustDB	PRK13820.
Enzyme and pathway databases
UniPathway	UPA00068; UER00113.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.90.1260.10. 1 hit.
HAMAP	MF_00005. Arg_succ_synth_type1.
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR11587. PTHR11587. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_METJA

Accession

Primary (citable) accession number: Q60174

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program