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Q60174 (ASSY_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:MJ0429
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148676

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1731Citrulline By similarity
Binding site1821Citrulline By similarity
Binding site2571Citrulline By similarity
Binding site2691Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60174 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 655D4A7AC06E7A7F

FASTA39544,723
        10         20         30         40         50         60 
MERIAVLAYS GGLDTSCCLK LLEDKYGYKV VSVCVDVGQP EEEIKEVEEK AKKLGVLKHY 

        70         80         90        100        110        120 
TIDAKEEFVK DYIFRAIKAN AMYEGYPLST ALARPLIAHK VVEIAEEVGA EAVAHGCTGK 

       130        140        150        160        170        180 
GNDQFRFETT IRIKAPHLKI IAPIRDLNLT RAEEIEYAKE KGIPIPTESK KYSIDENLWG 

       190        200        210        220        230        240 
RSIEGSELEN PDFVPPEEIY AWTKNPVEDK EEEIVEIEFK EGVPVAINGE KLEPVELIKK 

       250        260        270        280        290        300 
ANEIAGKHGV GRIDIIEDRI IGLKSRENYE CPGAVLLLTA HKALEQLVLT RDELRFKEIV 

       310        320        330        340        350        360 
DSLYGELIYK GLWFDPLRED LDAFIDKTQE RVTGTVKVKL FGGTARVVGR DSPYALYSKE 

       370        380        390 
LVSFDEKEID QKELAGMVKY HGLQAMLYEM RKKRK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98414.1.
PIRE64353.
RefSeqNP_247403.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ60174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0429.

Proteomic databases

PRIDEQ60174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98414; AAB98414; MJ_0429.
GeneID1451289.
KEGGmja:MJ_0429.

Phylogenomic databases

eggNOGCOG0137.
KOK01940.
OMAIYNGYWW.
ProtClustDBPRK13820.

Enzyme and pathway databases

UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_METJA
AccessionPrimary (citable) accession number: Q60174
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names