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Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.By similarity

GO - Molecular functioni

  • structural molecule activity Source: TIGR

GO - Biological processi

  • cell projection organization Source: TIGR
  • pathogenesis Source: TIGR

Enzyme and pathway databases

BioCyciVCHO:VC0828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene namesi
Name:tcpA
Ordered Locus Names:VC_0828
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  • extracellular organelle Source: InterPro
  • pilus Source: TIGR

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000241901 – 25Atypical leader peptideAdd BLAST25
ChainiPRO_000002419126 – 224Toxin coregulated pilinAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26N-methylmethioninePROSITE-ProRule annotation1
Disulfide bondi145 ↔ 211By similarity

Keywords - PTMi

Disulfide bond, Methylation

Miscellaneous databases

PMAP-CutDBiQ60153.

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0828.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 73Combined sources21
Turni74 – 76Combined sources3
Helixi85 – 97Combined sources13
Helixi103 – 106Combined sources4
Turni109 – 111Combined sources3
Beta strandi112 – 114Combined sources3
Beta strandi116 – 122Combined sources7
Beta strandi125 – 137Combined sources13
Helixi139 – 149Combined sources11
Helixi150 – 152Combined sources3
Beta strandi154 – 161Combined sources8
Helixi166 – 169Combined sources4
Turni172 – 174Combined sources3
Helixi179 – 181Combined sources3
Beta strandi183 – 187Combined sources5
Helixi201 – 206Combined sources6
Beta strandi210 – 212Combined sources3
Beta strandi214 – 220Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortaliQ60153.
SMRiQ60153.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60153.

Family & Domainsi

Domaini

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Phylogenomic databases

eggNOGiENOG4105XB6. Bacteria.
COG2165. LUCA.
KOiK10930.
OMAiQWDYVEA.

Family and domain databases

InterProiView protein in InterPro
IPR012902. N_methyl_site.
IPR010271. TcpA.
PfamiView protein in Pfam
PF07963. N_methyl. 1 hit.
PF05946. TcpA. 1 hit.
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiView protein in PROSITE
PS00409. PROKAR_NTER_METHYL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ
60 70 80 90 100
RAIDSQNMTK AAQNLNSVQI AMTQTYRSLG NYPATANANA ATQLANGLVS
110 120 130 140 150
LGKVSADEAK NPFTGTAMGI FSFPRNSAAN KAFAITVGGL TQAQCKTLVT
160 170 180 190 200
SVGDMFPFIN VKEGAFAAVA DLGDFETSVA DAATGAGVIK SIAPGSANLN
210 220
LTNITHVEKL CTGTAPFTVA FGNS
Length:224
Mass (Da):23,279
Last modified:November 1, 1996 - v1
Checksum:i7E871F63D52BC180
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRiJC4719.
RefSeqiNP_230476.1. NC_002505.1.
WP_001176374.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93991; AAF93991; VC_0828.
GeneIDi2614495.
KEGGivch:VC0828.
PATRICifig|243277.26.peg.789.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTCPA_VIBCH
AccessioniPrimary (citable) accession number: Q60153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references