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Q60153 (TCPA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene names
Name:tcpA
Ordered Locus Names:VC_0828
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium By similarity.

Subcellular location

Fimbrium By similarity.

Domain

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2525Atypical leader peptide
PRO_0000024190
Chain26 – 224199Toxin coregulated pilin
PRO_0000024191

Amino acid modifications

Modified residue261N-methylmethionine By similarity
Disulfide bond145 ↔ 211 By similarity

Secondary structure

1 .................................. 224
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q60153 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7E871F63D52BC180

FASTA22423,279
        10         20         30         40         50         60 
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ RAIDSQNMTK 

        70         80         90        100        110        120 
AAQNLNSVQI AMTQTYRSLG NYPATANANA ATQLANGLVS LGKVSADEAK NPFTGTAMGI 

       130        140        150        160        170        180 
FSFPRNSAAN KAFAITVGGL TQAQCKTLVT SVGDMFPFIN VKEGAFAAVA DLGDFETSVA 

       190        200        210        220 
DAATGAGVIK SIAPGSANLN LTNITHVEKL CTGTAPFTVA FGNS

« Hide

References

« Hide 'large scale' references
[1]"Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1."
Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A.
Gene 170:9-16(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: El Tor H1 / Serotype O1.
[2]"TcpA pilin sequences and colonization requirements for O1 and O139 vibrio cholerae."
Rhine J.A., Taylor R.K.
Mol. Microbiol. 13:1013-1020(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: El Tor H1 / Serotype O1.
[3]"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae."
Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. expand/collapse author list , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.
Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39315 / El Tor Inaba N16961.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRJC4719.
RefSeqNP_230476.1. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortalQ60153.
SMRQ60153. Positions 54-224.
ModBaseSearch...

Protein-protein interaction databases

STRING243277.VC0828.

Protocols and materials databases

DNASU2614495.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93991; AAF93991; VC_0828.
GeneID2614495.
KEGGvch:VC0828.
PATRIC20080761. VBIVibCho83274_0789.

Phylogenomic databases

eggNOGCOG2165.
KOK10930.
OMAFAMELNG.
ProtClustDBCLSK793805.

Family and domain databases

InterProIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamPF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ60153.
PMAP-CutDBQ60153.

Entry information

Entry nameTCPA_VIBCH
AccessionPrimary (citable) accession number: Q60153
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents