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Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.By similarity

GO - Molecular functioni

  1. structural molecule activity Source: TIGR

GO - Biological processi

  1. cell projection organization Source: TIGR
  2. pathogenesis Source: TIGR
Complete GO annotation...

Enzyme and pathway databases

BioCyciVCHO:VC0828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene namesi
Name:tcpA
Ordered Locus Names:VC_0828
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

Subcellular locationi

  1. Fimbrium By similarity

GO - Cellular componenti

  1. extracellular organelle Source: InterPro
  2. pilus Source: TIGR
Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2525Atypical leader peptidePRO_0000024190Add
BLAST
Chaini26 – 224199Toxin coregulated pilinPRO_0000024191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261N-methylmethioninePROSITE-ProRule annotation
Disulfide bondi145 ↔ 211By similarity

Keywords - PTMi

Disulfide bond, Methylation

Miscellaneous databases

PMAP-CutDBQ60153.

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0828.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi56 – 7621Combined sources
Turni77 – 793Combined sources
Helixi88 – 10013Combined sources
Helixi106 – 1094Combined sources
Turni112 – 1143Combined sources
Beta strandi115 – 1173Combined sources
Beta strandi119 – 1257Combined sources
Beta strandi128 – 14013Combined sources
Helixi142 – 15211Combined sources
Helixi153 – 1553Combined sources
Beta strandi157 – 1648Combined sources
Helixi169 – 1724Combined sources
Turni175 – 1773Combined sources
Helixi182 – 1843Combined sources
Beta strandi186 – 1905Combined sources
Helixi204 – 2096Combined sources
Beta strandi213 – 2153Combined sources
Beta strandi217 – 2237Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortaliQ60153.
SMRiQ60153. Positions 54-224.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60153.

Family & Domainsi

Domaini

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Phylogenomic databases

eggNOGiCOG2165.
KOiK10930.
OMAiGDFENSA.
OrthoDBiEOG680X19.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ
60 70 80 90 100
RAIDSQNMTK AAQNLNSVQI AMTQTYRSLG NYPATANANA ATQLANGLVS
110 120 130 140 150
LGKVSADEAK NPFTGTAMGI FSFPRNSAAN KAFAITVGGL TQAQCKTLVT
160 170 180 190 200
SVGDMFPFIN VKEGAFAAVA DLGDFETSVA DAATGAGVIK SIAPGSANLN
210 220
LTNITHVEKL CTGTAPFTVA FGNS
Length:224
Mass (Da):23,279
Last modified:November 1, 1996 - v1
Checksum:i7E871F63D52BC180
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRiJC4719.
RefSeqiNP_230476.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93991; AAF93991; VC_0828.
GeneIDi2614495.
KEGGivch:VC0828.
PATRICi20080761. VBIVibCho83274_0789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRiJC4719.
RefSeqiNP_230476.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortaliQ60153.
SMRiQ60153. Positions 54-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0828.

Protocols and materials databases

DNASUi2614495.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93991; AAF93991; VC_0828.
GeneIDi2614495.
KEGGivch:VC0828.
PATRICi20080761. VBIVibCho83274_0789.

Phylogenomic databases

eggNOGiCOG2165.
KOiK10930.
OMAiGDFENSA.
OrthoDBiEOG680X19.

Enzyme and pathway databases

BioCyciVCHO:VC0828-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ60153.
PMAP-CutDBQ60153.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1."
    Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A.
    Gene 170:9-16(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: El Tor H1 / Serotype O1.
  2. "TcpA pilin sequences and colonization requirements for O1 and O139 Vibrio cholerae."
    Rhine J.A., Taylor R.K.
    Mol. Microbiol. 13:1013-1020(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: El Tor H1 / Serotype O1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiTCPA_VIBCH
AccessioniPrimary (citable) accession number: Q60153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.