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Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.By similarity

GO - Molecular functioni

  • structural molecule activity Source: TIGR

GO - Biological processi

  • cell projection organization Source: TIGR
  • pathogenesis Source: TIGR
  • protein secretion by the type II secretion system Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

BioCyciVCHO:VC0828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene namesi
Name:tcpA
Ordered Locus Names:VC_0828
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  • extracellular organelle Source: InterPro
  • pilus Source: TIGR
Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000241901 – 25Atypical leader peptideAdd BLAST25
ChainiPRO_000002419126 – 224Toxin coregulated pilinAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26N-methylmethioninePROSITE-ProRule annotation1
Disulfide bondi145 ↔ 211By similarity

Keywords - PTMi

Disulfide bond, Methylation

Miscellaneous databases

PMAP-CutDBQ60153.

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0828.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi56 – 76Combined sources21
Turni77 – 79Combined sources3
Helixi88 – 100Combined sources13
Helixi106 – 109Combined sources4
Turni112 – 114Combined sources3
Beta strandi115 – 117Combined sources3
Beta strandi119 – 125Combined sources7
Beta strandi128 – 140Combined sources13
Helixi142 – 152Combined sources11
Helixi153 – 155Combined sources3
Beta strandi157 – 164Combined sources8
Helixi169 – 172Combined sources4
Turni175 – 177Combined sources3
Helixi182 – 184Combined sources3
Beta strandi186 – 190Combined sources5
Helixi204 – 209Combined sources6
Beta strandi213 – 215Combined sources3
Beta strandi217 – 223Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortaliQ60153.
SMRiQ60153.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60153.

Family & Domainsi

Domaini

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Phylogenomic databases

eggNOGiENOG4105XB6. Bacteria.
COG2165. LUCA.
KOiK10930.
OMAiGDFENSA.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF07963. N_methyl. 1 hit.
PF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ
60 70 80 90 100
RAIDSQNMTK AAQNLNSVQI AMTQTYRSLG NYPATANANA ATQLANGLVS
110 120 130 140 150
LGKVSADEAK NPFTGTAMGI FSFPRNSAAN KAFAITVGGL TQAQCKTLVT
160 170 180 190 200
SVGDMFPFIN VKEGAFAAVA DLGDFETSVA DAATGAGVIK SIAPGSANLN
210 220
LTNITHVEKL CTGTAPFTVA FGNS
Length:224
Mass (Da):23,279
Last modified:November 1, 1996 - v1
Checksum:i7E871F63D52BC180
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRiJC4719.
RefSeqiNP_230476.1. NC_002505.1.
WP_001176374.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93991; AAF93991; VC_0828.
GeneIDi2614495.
KEGGivch:VC0828.
PATRICi20080761. VBIVibCho83274_0789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74730 Genomic DNA. Translation: CAA52745.1.
U09807 Genomic DNA. Translation: AAA85786.1.
AE003852 Genomic DNA. Translation: AAF93991.1.
PIRiJC4719.
RefSeqiNP_230476.1. NC_002505.1.
WP_001176374.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HRVX-ray1.50A/B54-224[»]
ProteinModelPortaliQ60153.
SMRiQ60153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0828.

Protocols and materials databases

DNASUi2614495.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93991; AAF93991; VC_0828.
GeneIDi2614495.
KEGGivch:VC0828.
PATRICi20080761. VBIVibCho83274_0789.

Phylogenomic databases

eggNOGiENOG4105XB6. Bacteria.
COG2165. LUCA.
KOiK10930.
OMAiGDFENSA.

Enzyme and pathway databases

BioCyciVCHO:VC0828-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ60153.
PMAP-CutDBQ60153.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF07963. N_methyl. 1 hit.
PF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCPA_VIBCH
AccessioniPrimary (citable) accession number: Q60153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.