Reviewed, UniProtKB/Swiss-Prot Q60151 (GSHR_STRTR)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

gor

Organism

Streptococcus thermophilus

Taxonomic identifier

1308 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Glutathione reductase		PRO_0000067979
Regions
	Nucleotide binding	34 – 42	9	FAD By similarity
Sites
	Active site	439	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	42 ↔ 47		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60151-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6A0DA46BEA828804	FASTA	450	48,712
	10 20 30 40 50 60 MVKEYDYIVI GGGSGGIASA NRAAMHGAKV ILFEGKEVGG TCVNVGCVPK KVMWYGAQVA 70 80 90 100 110 120 ETLHRYAGEY GFDVTINNFD FATLKANRQA YIDRIHGSFE RGFDSNGVER VYEYARFVDP 130 140 150 160 170 180 HTVEVAGELY TAPHILIATG GHPLYPNIPG SEYGITSDGF FELDEVPKRT AVIGAGYIAV 190 200 210 220 230 240 EVAGVLNALG SDTHLFVRKD RPLRTFDKDI IDVLVDEMAK SGPTLHMHAN ATEVVKNADD 250 260 270 280 290 300 SLTISFDNEE TITVDCLIWA VGRAANTSGF GLEKTGVELT ERGNIYSDEF ENTSVPGIYA 310 320 330 340 350 360 LGDVTGKLDL TPVAVKAGRQ LSERLFNNKV DAKLDYTDVA TVVFSHPAIG AIGLTEEKAI 370 380 390 400 410 420 AKYGAENIKV YKSSFTPMYT ALGDNRQLST MKLVTLGEDE KIIGLHGIGY GVDEMIQGFS 430 440 450 VAIKMGATKA DFDNTVAIHP TGSEEFVTMR

References

[1]	"Characterization of the gor gene of the lactic acid bacterium Streptococcus thermophilus CNRZ368." Pebay M., Holl A.-C., Simonet J.-M., Decaris B. Res. Microbiol. 146:371-383(1995) [PubMed: 8525054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CNRZ 368.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27672 Genomic DNA. Translation: AAB00353.1. Z29494 Genomic DNA. Translation: CAA82630.1.
PIR	S41386.
3D structure databases
HSSP	HSSP built from PDB template 1ALG based on UniProtKB P00390.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glut_reduct_1. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_STRTR

Accession

Primary (citable) accession number: Q60151

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)