Q60140 (PLY_PSEVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectate lyase
Short name=PL
EC=4.2.2.2

Gene names

Name:

pel

Organism

Pseudomonas viridiflava

Taxonomic identifier

33069 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a role in bacterial invasion of plants.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 380	351	Pectate lyase		PRO_0000024863
Sites
	Active site	264	1	Potential
	Metal binding	169	1	Calcium By similarity
	Metal binding	208	1	Calcium By similarity
	Metal binding	212	1	Calcium By similarity
Experimental info
	Sequence conflict	297	1	I → V in AAC41521. Ref.1
	Sequence conflict	380	1	L → DSRGS in AAC41521. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q60140 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BB4ABC2B715D51C6	FASTA	380	40,384
	10 20 30 40 50 60 MVKPSLFSAN KLASAVVASL LFASAGAQAD IATDVATTGW ATQNGGTKGG SKAAANNIYT 70 80 90 100 110 120 VKNAAELKAA LKASVGTNGR IIKISGVIDV SEGNAYTKTA DMKARGRLDI PGKTTIVGIT 130 140 150 160 170 180 NNAEIREGFF YAKENDVIIR NLTIENPWDP EPIWDANDGS AGNWNSEYDG LTIEGANNVW 190 200 210 220 230 240 VDHVTFTDGR RTDDQNGTAN GRPKQHHDGA LDVKNGANYV TISYTAFKSH EKNNLIGSSD 250 260 270 280 290 300 SRTTDDGKLK VTIHNSLFEN ISARAPRVRF GQVHLYNNYH VGSASHSVYP FSYAHGIGKS 310 320 330 340 350 360 SKIFSEKNAF EISGISGCTK IAGDYGGSVY RDSGSTLNGT ALTCTWSSSI GWTPPYSYTP 370 380 LNADKVKADV TAKAGAGKIL

References

[1]	"Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas." Liao C.H., Gaffney T.D., Bradley S.P., Wong L.C. Mol. Plant Microbe Interact. 9:14-21(1996) [PubMed: 8589419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SJ074.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38901 Genomic DNA. Translation: AAB46398.1. D44611 Genomic DNA. Translation: BAA08077.1. L38574 Genomic DNA. Translation: AAC41521.1.
3D structure databases
ProteinModelPortal	Q60140.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY_PSEVI

Accession

Primary (citable) accession number: Q60140
Secondary accession number(s): Q52619

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program