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Q60132

- T2C9_CITFR

UniProt

Q60132 - T2C9_CITFR

Protein

Type-2 restriction enzyme Cfr9I

Gene

cfr9IR

Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 44 (16 Apr 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi699. Cfr9I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme Cfr9I (EC:3.1.21.4)
    Short name:
    R.Cfr9I
    Alternative name(s):
    Endonuclease Cfr9I
    Type II restriction enzyme Cfr9I
    Gene namesi
    Name:cfr9IR
    OrganismiCitrobacter freundii
    Taxonomic identifieri546 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Type-2 restriction enzyme Cfr9IPRO_0000077294Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019071. Restrct_endonuc_II_XcyI.
    [Graphical view]
    PfamiPF09571. RE_XcyI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60132-1 [UniParc]FASTAAdd to Basket

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    MTNKIVFPEP KQQVDFAFSL KRFRGIYLQN ALLETVRDMD IVALDTQLAE    50
    YVNKADLATL ATYGLRAELL FPVPVLLETN PFLLGYYRLL MGYSQKEFYG 100
    KDKGFNAGCF KSMEVKGNIG KVAKPKISEL CHAFCSVASS LLQGVGPLRI 150
    SRELLDDLTL LTVGPQLRGG ANNQRGADGI VLVFEIIKEI VSHAVAEVRE 200
    NAIEVNSATG RNVLIEFAPD PDIIIREEMS LDNYRNVVAI EVKSGTDVSN 250
    IHNRIGEAEK SHQKARGHGY TECWTVVNVS RLDMDKARKE SPSTNRFYSI 300
    TDLSLREGEQ YEDFRRRVLS LTAISAAPTP 330
    Length:330
    Mass (Da):36,806
    Last modified:November 1, 1996 - v1
    Checksum:i6DD85FF9DA98117E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17022 Genomic DNA. Translation: CAA34888.1.
    X74517 Genomic DNA. Translation: CAA52628.1.
    PIRiI40700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17022 Genomic DNA. Translation: CAA34888.1 .
    X74517 Genomic DNA. Translation: CAA52628.1 .
    PIRi I40700.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 699. Cfr9I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019071. Restrct_endonuc_II_XcyI.
    [Graphical view ]
    Pfami PF09571. RE_XcyI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system."
      Lubys A., Menkevicius S., Timinskas A., Butkus V., Janulaitis A.
      Gene 141:85-89(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RFL9.

    Entry informationi

    Entry nameiT2C9_CITFR
    AccessioniPrimary (citable) accession number: Q60132
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: April 16, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3