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Q60132 (T2C9_CITFR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme Cfr9I

Short name=R.Cfr9I
EC=3.1.21.4
Alternative name(s):
Endonuclease Cfr9I
Type II restriction enzyme Cfr9I
Gene names
Name:cfr9IR
OrganismCitrobacter freundii
Taxonomic identifier546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Type-2 restriction enzyme Cfr9I
PRO_0000077294

Sequences

Sequence LengthMass (Da)Tools
Q60132 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6DD85FF9DA98117E

FASTA33036,806
        10         20         30         40         50         60 
MTNKIVFPEP KQQVDFAFSL KRFRGIYLQN ALLETVRDMD IVALDTQLAE YVNKADLATL 

        70         80         90        100        110        120 
ATYGLRAELL FPVPVLLETN PFLLGYYRLL MGYSQKEFYG KDKGFNAGCF KSMEVKGNIG 

       130        140        150        160        170        180 
KVAKPKISEL CHAFCSVASS LLQGVGPLRI SRELLDDLTL LTVGPQLRGG ANNQRGADGI 

       190        200        210        220        230        240 
VLVFEIIKEI VSHAVAEVRE NAIEVNSATG RNVLIEFAPD PDIIIREEMS LDNYRNVVAI 

       250        260        270        280        290        300 
EVKSGTDVSN IHNRIGEAEK SHQKARGHGY TECWTVVNVS RLDMDKARKE SPSTNRFYSI 

       310        320        330 
TDLSLREGEQ YEDFRRRVLS LTAISAAPTP 

« Hide

References

[1]"Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system."
Lubys A., Menkevicius S., Timinskas A., Butkus V., Janulaitis A.
Gene 141:85-89(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RFL9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17022 Genomic DNA. Translation: CAA34888.1.
X74517 Genomic DNA. Translation: CAA52628.1.
PIRI40700.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE699. Cfr9I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019071. Restrct_endonuc_II_XcyI.
[Graphical view]
PfamPF09571. RE_XcyI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2C9_CITFR
AccessionPrimary (citable) accession number: Q60132
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries