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Protein

Type-2 restriction enzyme Cfr9I

Gene

cfr9IR

Organism
Citrobacter freundii
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Protein family/group databases

REBASEi699. Cfr9I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Cfr9I (EC:3.1.21.4)
Short name:
R.Cfr9I
Alternative name(s):
Endonuclease Cfr9I
Type II restriction enzyme Cfr9I
Gene namesi
Name:cfr9IR
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772941 – 330Type-2 restriction enzyme Cfr9IAdd BLAST330

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR019071. Restrct_endonuc_II_XcyI.
PfamiView protein in Pfam
PF09571. RE_XcyI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKIVFPEP KQQVDFAFSL KRFRGIYLQN ALLETVRDMD IVALDTQLAE
60 70 80 90 100
YVNKADLATL ATYGLRAELL FPVPVLLETN PFLLGYYRLL MGYSQKEFYG
110 120 130 140 150
KDKGFNAGCF KSMEVKGNIG KVAKPKISEL CHAFCSVASS LLQGVGPLRI
160 170 180 190 200
SRELLDDLTL LTVGPQLRGG ANNQRGADGI VLVFEIIKEI VSHAVAEVRE
210 220 230 240 250
NAIEVNSATG RNVLIEFAPD PDIIIREEMS LDNYRNVVAI EVKSGTDVSN
260 270 280 290 300
IHNRIGEAEK SHQKARGHGY TECWTVVNVS RLDMDKARKE SPSTNRFYSI
310 320 330
TDLSLREGEQ YEDFRRRVLS LTAISAAPTP
Length:330
Mass (Da):36,806
Last modified:November 1, 1996 - v1
Checksum:i6DD85FF9DA98117E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17022 Genomic DNA. Translation: CAA34888.1.
X74517 Genomic DNA. Translation: CAA52628.1.
PIRiI40700.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2C9_CITFR
AccessioniPrimary (citable) accession number: Q60132
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries