Reviewed, UniProtKB/Swiss-Prot Q60114 (SACB_ZYMMO)

Names and origin

Protein names

Recommended name:
    Levansucrase
    EC=2.4.1.10
Alternative name(s):
    Beta-D-fructofuranosyl transferase
    Sucrose 6-fructosyl transferase

Gene names

Name:	sacB
Synonyms:	levU, sucE2
Ordered Locus Names:	ZMO0374

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside.
Subcellular location	Secreted.
Post-translational modification	Does not seem to be N-terminally processed.
Sequence similarities	Belongs to the glycosyl hydrolase 68 family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	sugar utilization Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	levansucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Levansucrase		PRO_0000057721
Experimental info
	Sequence conflict	39	1	V → I in BAA04475. Ref.2
	Sequence conflict	200 – 203	4	NPED → TPKI Ref.1
	Sequence conflict	200 – 203	4	NPED → TPKI Ref.4
	Sequence conflict	217	1	E → Q in BAA04475. Ref.2
	Sequence conflict	220	1	T → A in BAA04475. Ref.2
	Sequence conflict	244	1	C → Y in BAA04475. Ref.2
	Sequence conflict	379	1	I → V in BAA04475. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q60114-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 19A4691DA3EB9FE3	FASTA	423	46,762
	10 20 30 40 50 60 MLNKAGIAEP SLWTRADAMK VHTDDPTATM PTIDYDFPVM TDKYWVWDTW PLRDINGQVV 70 80 90 100 110 120 SFQGWSVIFA LVADRTKYGW HNRNDGARIG YFYSRGGSNW IFGGHLLKDG ANPRSWEWSG 130 140 150 160 170 180 CTIMAPGTAN SVEVFFTSVN DTPSESVPAQ CKGYIYADDK SVWFDGFDKV TDLFQADGLY 190 200 210 220 230 240 YADYAENNFW DFRDPHVFIN PEDGKTYALF EGNVAMERGT VAVGEEEIGP VPPKTETPDG 250 260 270 280 290 300 ARYCAAAIGI AQALNEARTE WKLLPPLVTA FGVNDQTERP HVVFQNGLTY LFTISHHSTY 310 320 330 340 350 360 ADGLSGPDGV YGFVSENGIF GPYEPLNGSG LVLGNPSSQP YQAYSHYVMT NGLVTSFIDT 370 380 390 400 410 420 IPSSDPNVYR YGGTLAPTIK LELVGHRSFV TEVKGYGYIP PQIEWLAEDE SSNSAAALSL LNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 (ATCC10988)." Song K.B., Joo H.K., Rhee S.-K. Biochim. Biophys. Acta 1173:320-324(1993) [PubMed: 8318541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10988 / NCIB 8938 / NRRL B-806 / ZM1.
[2]	"Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase." Kyono K., Yanase H., Tonomura K., Kawasaki H., Sakai T. Biosci. Biotechnol. Biochem. 59:289-293(1995) [PubMed: 7766026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 29191 / DSM 3580 / IFO 13756 / NCIB 11199 / ZM6.
[3]	Gunasekaran P., Mukundan G., Kannan R., Velmurugan S., Ait-Abdelkader N., Alvarez E., Baratti J. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10988 / NCIB 8938 / NRRL B-806 / ZM1.
[4]	"Sequence analysis of 44B6 fosmid clone of Zymomonas mobilis ZM4." Ahn J.Y., Kang H.S. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[5]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF081588 Genomic DNA. Translation: AAA27695.1. D17524 Genomic DNA. Translation: BAA04475.1. L33402 Genomic DNA. Translation: AAA27702.1. AF313764 Genomic DNA. Translation: AAG29870.1. AE008692 Genomic DNA. Translation: AAV88998.1.
PIR	JC2519. S33771.
RefSeq	YP_162109.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH68. Glycoside Hydrolase Family 68.
Genome annotation databases
GeneID	3188627.
GenomeReviews	Gene locus ZMO0374 in contig AE008692_GR.
KEGG	zmo:ZMO0374.
NMPDR	fig|264203.3.peg.870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q60114.
OMA	Q60114. TRADAKQ.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO0374-MON.
BRENDA	2.4.1.10. 1658.
Family and domain databases
InterPro	IPR003469. Glyco_hydro_68. [Graphical view]
Pfam	PF02435. Glyco_hydro_68. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SACB_ZYMMO

Accession

Primary (citable) accession number: Q60114
Secondary accession number(s): Q06487, Q5NQK6, Q60116

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)