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Q60106 (XANP_XANS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xanthomonalisin

EC=3.4.21.101
Alternative name(s):
Carboxyl proteinase
XCP
Xanthomonapepsin
Xanthomonas aspartic proteinase
OrganismXanthomonas sp. (strain T-22)
Taxonomic identifier136420 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Cleavage of casein.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secreted.

Post-translational modification

Autocatalytically processed.

Sequence similarities

Contains 1 peptidase S53 domain.

Contains 1 PKD domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMZymogen
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 237214Removed in mature form
PRO_0000027365
Chain238 – 635398Xanthomonalisin
PRO_0000027366
Propeptide636 – 827192Removed in mature form
PRO_0000027367

Regions

Domain241 – 625385Peptidase S53
Domain635 – 72288PKD

Sites

Active site3121Charge relay system By similarity
Active site3161Charge relay system By similarity
Active site5441Charge relay system By similarity
Metal binding5851Calcium By similarity
Metal binding5861Calcium; via carbonyl oxygen By similarity
Metal binding6031Calcium; via carbonyl oxygen By similarity
Metal binding6051Calcium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60106 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 21A33C4C683DBC8F

FASTA82783,707
        10         20         30         40         50         60 
MKIEKTALTV AIALAMSSLS AHAEDAWVST HTQAAMSPPA STQVLAASST SATTTGNAYT 

        70         80         90        100        110        120 
LNMTGSPRID GAAVTALEAD HPLHVEVALK LRNPDALQTF LAGVTTPGSA LFGKFLTPSQ 

       130        140        150        160        170        180 
FTERFGPTQS QVDAVVAHLQ QAGFTNIEVA PNRLLISADG TAGAATNGFR TSIKRFSANG 

       190        200        210        220        230        240 
REFFANDAPA LVPASLGDSV NAVLGLQNVS VKHTLHHVYH PEDVTVPGPN VGTQAAAAVA 

       250        260        270        280        290        300 
AHHPQDFAAI YGGSSLPAAT NTAVGIITWG SITQTVTDLN SFTSGAGLAT VNSTITKVGS 

       310        320        330        340        350        360 
GTFANDPDSN GEWSLDSQDI VGIAGGVKQL IFYTSANGDS SSSGITDAGI TASYNRAVTD 

       370        380        390        400        410        420 
NIAKLINVSL GEDETAAQQS GTQAADDAIF QQAVAQGQTF SIASGDAGVY QWSTDPTSGS 

       430        440        450        460        470        480 
PGYVANSAGT VKIDLTHYSV SEPASSPYVI QVGGTTLSTS GTTWSGETVW NEGLSAIAPS 

       490        500        510        520        530        540 
QGDNNQRLWA TGGGVSLYEA APSWQSSVSS STKRVGPDLA FDAASSSGAL IVVNGSTEQV 

       550        560        570        580        590        600 
GGTSLASPLF VGAFARIESA ANNAIGFPAS KFYQAFPTQT SLLHDVTSGN NGYQSHGYTA 

       610        620        630        640        650        660 
ATGFDEATGF GSFDIGKLNT YAQANWVTGG GGGSTNAPPV ANFSVATTGL VATFTDSSTD 

       670        680        690        700        710        720 
SDGSIASHAW TFGDGSTSTA TSPSHTYSAA GTYSVAETVT DNAGATSTKT SSVTVSSSGG 

       730        740        750        760        770        780 
TGGGTVLQNG VAATGLSAAK NGQLKYTVAI PSGAKSLKIA ISGGTGDADL YVKFGSAPTT 

       790        800        810        820 
SSYDCRPYVT GNTESCSFAS PQTGTYYVLL NGYAAFSGVS LKATWTN 

« Hide

References

[1]"Cloning and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from Xanthomonas sp. T-22."
Oda K., Ito M., Uchida K., Shibano Y., Fukuhara K., Takahashi S.
J. Biochem. 120:564-572(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis."
Oyama H., Abe S., Ushiyama S., Takahashi S., Oda K.
J. Biol. Chem. 274:27815-27822(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASPARTIC ACID AND GLUTAMIC ACID RESIDUES, ACTIVE SITES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83740 Genomic DNA. Translation: BAA12093.1.

3D structure databases

ProteinModelPortalQ60106.
SMRQ60106. Positions 241-625.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS53.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.4.21.101. 6720.

Family and domain databases

Gene3D2.60.40.670. 1 hit.
3.40.50.200. 1 hit.
InterProIPR007280. Peptidase_C_arc/bac.
IPR015366. Peptidase_S53_propep.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamPF00801. PKD. 1 hit.
PF04151. PPC. 1 hit.
PF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTSM00089. PKD. 1 hit.
SM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMSSF49299. SSF49299. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEPS50093. PKD. 1 hit.
PS51695. SEDOLISIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXANP_XANS2
AccessionPrimary (citable) accession number: Q60106
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries