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Q60099

- HAD_XANAU

UniProt

Q60099 - HAD_XANAU

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Protein

(S)-2-haloacid dehalogenase

Gene

dhlB

Organism
Xanthobacter autotrophicus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Active with 2-halogenated carboxylic acids and converts only the L-isomer of 2-chloropropionic acid with inversion of configuration to produce D-lactate.

Catalytic activityi

(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.

pH dependencei

Optimum pH is 9.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81Nucleophile

GO - Molecular functioni

  1. (S)-2-haloacid dehalogenase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-haloacid dehalogenase (EC:3.8.1.2)
Alternative name(s):
2-haloalkanoic acid dehalogenase
Halocarboxylic acid halidohydrolase
L-2-haloacid dehalogenase
Gene namesi
Name:dhlB
OrganismiXanthobacter autotrophicus
Taxonomic identifieri280 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253(S)-2-haloacid dehalogenasePRO_0000079159Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Turni11 – 133Combined sources
Turni16 – 194Combined sources
Helixi20 – 267Combined sources
Helixi31 – 5222Combined sources
Helixi58 – 7215Combined sources
Helixi79 – 868Combined sources
Helixi87 – 904Combined sources
Helixi98 – 1058Combined sources
Beta strandi108 – 1169Combined sources
Helixi118 – 12710Combined sources
Helixi131 – 1333Combined sources
Beta strandi135 – 1395Combined sources
Helixi140 – 1434Combined sources
Helixi150 – 16011Combined sources
Helixi164 – 1663Combined sources
Beta strandi167 – 1726Combined sources
Helixi174 – 18310Combined sources
Beta strandi186 – 1905Combined sources
Helixi195 – 2017Combined sources
Beta strandi204 – 2063Combined sources
Helixi209 – 2179Combined sources
Beta strandi228 – 2336Combined sources
Helixi234 – 2363Combined sources
Helixi237 – 2448Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AQ6X-ray1.95A/B1-253[»]
1QQ5X-ray1.52A/B1-253[»]
1QQ6X-ray2.10A/B1-253[»]
1QQ7X-ray1.70A/B1-253[»]
ProteinModelPortaliQ60099.
SMRiQ60099. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60099.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00413. HADHALOGNASE.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR01428. HAD_type_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60099-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKAVVFDAY GTLFDVQSVA DATERAYPGR GEYITQVWRQ KQLEYSWLRA
60 70 80 90 100
LMGRYADFWG VTREALAYTL GTLGLEPDES FLADMAQAYN RLTPYPDAAQ
110 120 130 140 150
CLAELAPLKR AILSNGAPDM LQALVANAGL TDSFDAVISV DAKRVFKPHP
160 170 180 190 200
DSYALVEEVL GVTPAEVLFV SSNGFDVGGA KNFGFSVARV ARLSQEALAR
210 220 230 240 250
ELVSGTIAPL TMFKALRMRE ETYAEAPDFV VPALGDLPRL VRGMAGAHLA

PAV
Length:253
Mass (Da):27,469
Last modified:July 15, 1998 - v2
Checksum:iE2AB8DEED37A5716
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841D → G(PubMed:1744048)Curated
Sequence conflicti84 – 841D → G(PubMed:7580000)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81691 Genomic DNA. Translation: AAA27590.1.
X86084 Genomic DNA. Translation: CAA60039.1.
PIRiS52840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81691 Genomic DNA. Translation: AAA27590.1 .
X86084 Genomic DNA. Translation: CAA60039.1 .
PIRi S52840.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AQ6 X-ray 1.95 A/B 1-253 [» ]
1QQ5 X-ray 1.52 A/B 1-253 [» ]
1QQ6 X-ray 2.10 A/B 1-253 [» ]
1QQ7 X-ray 1.70 A/B 1-253 [» ]
ProteinModelPortali Q60099.
SMRi Q60099. Positions 1-245.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q60099.

Family and domain databases

Gene3Di 1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProi IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view ]
Pfami PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00413. HADHALOGNASE.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR01428. HAD_type_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene."
    van der Ploeg J., van Hall G., Janssen D.B.
    J. Bacteriol. 173:7925-7933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
    Strain: GJ10.
  2. "Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10."
    van der Ploeg J., Janssen D.B.
    Biodegradation 6:257-263(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
    Strain: GJ10.
  3. "Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate."
    Ridder I.S., Rozeboom H.J., Kalk K.H., Janssen D.B., Dijkstra B.W.
    J. Biol. Chem. 272:33015-33022(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), SEQUENCE REVISION TO 84.
    Strain: GJ10.

Entry informationi

Entry nameiHAD_XANAU
AccessioniPrimary (citable) accession number: Q60099
Secondary accession number(s): Q56757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 26, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3