SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q60099

- HAD_XANAU

UniProt

Q60099 - HAD_XANAU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

(S)-2-haloacid dehalogenase

Gene
dhlB
Organism
Xanthobacter autotrophicus
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Active with 2-halogenated carboxylic acids and converts only the L-isomer of 2-chloropropionic acid with inversion of configuration to produce D-lactate.

Catalytic activityi

(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.

pH dependencei

Optimum pH is 9.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81Nucleophile

GO - Molecular functioni

  1. (S)-2-haloacid dehalogenase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-haloacid dehalogenase (EC:3.8.1.2)
Alternative name(s):
2-haloalkanoic acid dehalogenase
Halocarboxylic acid halidohydrolase
L-2-haloacid dehalogenase
Gene namesi
Name:dhlB
OrganismiXanthobacter autotrophicus
Taxonomic identifieri280 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253(S)-2-haloacid dehalogenasePRO_0000079159Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Turni11 – 133
Turni16 – 194
Helixi20 – 267
Helixi31 – 5222
Helixi58 – 7215
Helixi79 – 868
Helixi87 – 904
Helixi98 – 1058
Beta strandi108 – 1169
Helixi118 – 12710
Helixi131 – 1333
Beta strandi135 – 1395
Helixi140 – 1434
Helixi150 – 16011
Helixi164 – 1663
Beta strandi167 – 1726
Helixi174 – 18310
Beta strandi186 – 1905
Helixi195 – 2017
Beta strandi204 – 2063
Helixi209 – 2179
Beta strandi228 – 2336
Helixi234 – 2363
Helixi237 – 2448

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AQ6X-ray1.95A/B1-253[»]
1QQ5X-ray1.52A/B1-253[»]
1QQ6X-ray2.10A/B1-253[»]
1QQ7X-ray1.70A/B1-253[»]
ProteinModelPortaliQ60099.
SMRiQ60099. Positions 1-245.

Miscellaneous databases

EvolutionaryTraceiQ60099.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00413. HADHALOGNASE.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR01428. HAD_type_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60099-1 [UniParc]FASTAAdd to Basket

« Hide

MIKAVVFDAY GTLFDVQSVA DATERAYPGR GEYITQVWRQ KQLEYSWLRA    50
LMGRYADFWG VTREALAYTL GTLGLEPDES FLADMAQAYN RLTPYPDAAQ 100
CLAELAPLKR AILSNGAPDM LQALVANAGL TDSFDAVISV DAKRVFKPHP 150
DSYALVEEVL GVTPAEVLFV SSNGFDVGGA KNFGFSVARV ARLSQEALAR 200
ELVSGTIAPL TMFKALRMRE ETYAEAPDFV VPALGDLPRL VRGMAGAHLA 250
PAV 253
Length:253
Mass (Da):27,469
Last modified:July 15, 1998 - v2
Checksum:iE2AB8DEED37A5716
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841D → G1 Publication
Sequence conflicti84 – 841D → G1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81691 Genomic DNA. Translation: AAA27590.1.
X86084 Genomic DNA. Translation: CAA60039.1.
PIRiS52840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81691 Genomic DNA. Translation: AAA27590.1 .
X86084 Genomic DNA. Translation: CAA60039.1 .
PIRi S52840.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AQ6 X-ray 1.95 A/B 1-253 [» ]
1QQ5 X-ray 1.52 A/B 1-253 [» ]
1QQ6 X-ray 2.10 A/B 1-253 [» ]
1QQ7 X-ray 1.70 A/B 1-253 [» ]
ProteinModelPortali Q60099.
SMRi Q60099. Positions 1-245.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q60099.

Family and domain databases

Gene3Di 1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProi IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view ]
Pfami PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00413. HADHALOGNASE.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR01428. HAD_type_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene."
    van der Ploeg J., van Hall G., Janssen D.B.
    J. Bacteriol. 173:7925-7933(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
    Strain: GJ10.
  2. "Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10."
    van der Ploeg J., Janssen D.B.
    Biodegradation 6:257-263(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
    Strain: GJ10.
  3. "Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate."
    Ridder I.S., Rozeboom H.J., Kalk K.H., Janssen D.B., Dijkstra B.W.
    J. Biol. Chem. 272:33015-33022(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), SEQUENCE REVISION TO 84.
    Strain: GJ10.

Entry informationi

Entry nameiHAD_XANAU
AccessioniPrimary (citable) accession number: Q60099
Secondary accession number(s): Q56757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 16, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi