Q60099 (HAD_XANAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(S)-2-haloacid dehalogenase
EC=3.8.1.2

Alternative name(s):
2-haloalkanoic acid dehalogenase
Halocarboxylic acid halidohydrolase
L-2-haloacid dehalogenase

Gene names

Name:

dhlB

Organism

Xanthobacter autotrophicus

Taxonomic identifier

280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Xanthobacter

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Active with 2-halogenated carboxylic acids and converts only the L-isomer of 2-chloropropionic acid with inversion of configuration to produce D-lactate.
Catalytic activity	(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.5.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	(S)-2-haloacid dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 253

253

(S)-2-haloacid dehalogenase

PRO_0000079159

Sites

Active site

8

1

Nucleophile

Experimental info

Sequence conflict

84

1

D → G Ref.1

Sequence conflict

84

1

D → G Ref.2

Secondary structure

1

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253

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Turn

11 – 13

3

Turn

16 – 19

4

Helix

20 – 26

7

Helix

31 – 52

22

Helix

58 – 72

15

Helix

79 – 86

8

Helix

87 – 90

4

Helix

98 – 105

8

Beta strand

108 – 116

9

Helix

118 – 127

10

Helix

131 – 133

3

Beta strand

135 – 139

5

Helix

140 – 143

4

Helix

150 – 160

11

Helix

164 – 166

3

Beta strand

167 – 172

6

Helix

174 – 183

10

Beta strand

186 – 190

5

Helix

195 – 201

7

Beta strand

204 – 206

3

Helix

209 – 217

9

Beta strand

228 – 233

6

Helix

234 – 236

3

Helix

237 – 244

8

Sequences

	Sequence		Length	Mass (Da)
	Q60099 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: E2AB8DEED37A5716	FASTA	253	27,469
	10 20 30 40 50 60 MIKAVVFDAY GTLFDVQSVA DATERAYPGR GEYITQVWRQ KQLEYSWLRA LMGRYADFWG 70 80 90 100 110 120 VTREALAYTL GTLGLEPDES FLADMAQAYN RLTPYPDAAQ CLAELAPLKR AILSNGAPDM 130 140 150 160 170 180 LQALVANAGL TDSFDAVISV DAKRVFKPHP DSYALVEEVL GVTPAEVLFV SSNGFDVGGA 190 200 210 220 230 240 KNFGFSVARV ARLSQEALAR ELVSGTIAPL TMFKALRMRE ETYAEAPDFV VPALGDLPRL 250 VRGMAGAHLA PAV

References

[1]	"Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene." van der Ploeg J., van Hall G., Janssen D.B. J. Bacteriol. 173:7925-7933(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18. Strain: GJ10.
[2]	"Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10." van der Ploeg J., Janssen D.B. Biodegradation 6:257-263(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122. Strain: GJ10.
[3]	"Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate." Ridder I.S., Rozeboom H.J., Kalk K.H., Janssen D.B., Dijkstra B.W. J. Biol. Chem. 272:33015-33022(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), SEQUENCE REVISION TO 84. Strain: GJ10.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M81691 Genomic DNA. Translation: AAA27590.1.
X86084 Genomic DNA. Translation: CAA60039.1.

PIR

S52840.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AQ6	X-ray	1.95	A/B	1-253	[»]
1QQ5	X-ray	1.52	A/B	1-253	[»]
1QQ6	X-ray	2.10	A/B	1-253	[»]
1QQ7	X-ray	1.70	A/B	1-253	[»]

ProteinModelPortal

Q60099.

SMR

Q60099. Positions 1-245.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.

InterPro

IPR023214. HAD-like_dom.
IPR006388. HAD-SF_hydro_IA_v2.
IPR006402. HAD-SF_hydro_IA_v3.
IPR006328. HAD_II.
IPR005833. Haloacid_DH/epoxide_hydro.
IPR023198. PGP_dom2.
[Graphical view]

Pfam

PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00413. HADHALOGNASE.

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR01428. HAD_type_II. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q60099.

Entry information

Entry name

HAD_XANAU

Accession

Primary (citable) accession number: Q60099
Secondary accession number(s): Q56757

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program