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Q60099

- HAD_XANAU

UniProt

Q60099 - HAD_XANAU

Protein

(S)-2-haloacid dehalogenase

Gene

dhlB

Organism
Xanthobacter autotrophicus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Active with 2-halogenated carboxylic acids and converts only the L-isomer of 2-chloropropionic acid with inversion of configuration to produce D-lactate.

    Catalytic activityi

    (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.

    pH dependencei

    Optimum pH is 9.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei8 – 81Nucleophile

    GO - Molecular functioni

    1. (S)-2-haloacid dehalogenase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (S)-2-haloacid dehalogenase (EC:3.8.1.2)
    Alternative name(s):
    2-haloalkanoic acid dehalogenase
    Halocarboxylic acid halidohydrolase
    L-2-haloacid dehalogenase
    Gene namesi
    Name:dhlB
    OrganismiXanthobacter autotrophicus
    Taxonomic identifieri280 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 253253(S)-2-haloacid dehalogenasePRO_0000079159Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    253
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Turni11 – 133
    Turni16 – 194
    Helixi20 – 267
    Helixi31 – 5222
    Helixi58 – 7215
    Helixi79 – 868
    Helixi87 – 904
    Helixi98 – 1058
    Beta strandi108 – 1169
    Helixi118 – 12710
    Helixi131 – 1333
    Beta strandi135 – 1395
    Helixi140 – 1434
    Helixi150 – 16011
    Helixi164 – 1663
    Beta strandi167 – 1726
    Helixi174 – 18310
    Beta strandi186 – 1905
    Helixi195 – 2017
    Beta strandi204 – 2063
    Helixi209 – 2179
    Beta strandi228 – 2336
    Helixi234 – 2363
    Helixi237 – 2448

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AQ6X-ray1.95A/B1-253[»]
    1QQ5X-ray1.52A/B1-253[»]
    1QQ6X-ray2.10A/B1-253[»]
    1QQ7X-ray1.70A/B1-253[»]
    ProteinModelPortaliQ60099.
    SMRiQ60099. Positions 1-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60099.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.150.240. 1 hit.
    3.40.50.1000. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR006328. HAD_II.
    IPR023198. PGP_dom2.
    [Graphical view]
    PfamiPF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01493. HAD-SF-IA-v2. 1 hit.
    TIGR01509. HAD-SF-IA-v3. 1 hit.
    TIGR01428. HAD_type_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q60099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKAVVFDAY GTLFDVQSVA DATERAYPGR GEYITQVWRQ KQLEYSWLRA    50
    LMGRYADFWG VTREALAYTL GTLGLEPDES FLADMAQAYN RLTPYPDAAQ 100
    CLAELAPLKR AILSNGAPDM LQALVANAGL TDSFDAVISV DAKRVFKPHP 150
    DSYALVEEVL GVTPAEVLFV SSNGFDVGGA KNFGFSVARV ARLSQEALAR 200
    ELVSGTIAPL TMFKALRMRE ETYAEAPDFV VPALGDLPRL VRGMAGAHLA 250
    PAV 253
    Length:253
    Mass (Da):27,469
    Last modified:July 15, 1998 - v2
    Checksum:iE2AB8DEED37A5716
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841D → G(PubMed:1744048)Curated
    Sequence conflicti84 – 841D → G(PubMed:7580000)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81691 Genomic DNA. Translation: AAA27590.1.
    X86084 Genomic DNA. Translation: CAA60039.1.
    PIRiS52840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81691 Genomic DNA. Translation: AAA27590.1 .
    X86084 Genomic DNA. Translation: CAA60039.1 .
    PIRi S52840.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AQ6 X-ray 1.95 A/B 1-253 [» ]
    1QQ5 X-ray 1.52 A/B 1-253 [» ]
    1QQ6 X-ray 2.10 A/B 1-253 [» ]
    1QQ7 X-ray 1.70 A/B 1-253 [» ]
    ProteinModelPortali Q60099.
    SMRi Q60099. Positions 1-245.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q60099.

    Family and domain databases

    Gene3Di 1.10.150.240. 1 hit.
    3.40.50.1000. 1 hit.
    InterProi IPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR006328. HAD_II.
    IPR023198. PGP_dom2.
    [Graphical view ]
    Pfami PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00413. HADHALOGNASE.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01493. HAD-SF-IA-v2. 1 hit.
    TIGR01509. HAD-SF-IA-v3. 1 hit.
    TIGR01428. HAD_type_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene."
      van der Ploeg J., van Hall G., Janssen D.B.
      J. Bacteriol. 173:7925-7933(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
      Strain: GJ10.
    2. "Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10."
      van der Ploeg J., Janssen D.B.
      Biodegradation 6:257-263(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
      Strain: GJ10.
    3. "Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate."
      Ridder I.S., Rozeboom H.J., Kalk K.H., Janssen D.B., Dijkstra B.W.
      J. Biol. Chem. 272:33015-33022(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), SEQUENCE REVISION TO 84.
      Strain: GJ10.

    Entry informationi

    Entry nameiHAD_XANAU
    AccessioniPrimary (citable) accession number: Q60099
    Secondary accession number(s): Q56757
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3