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Q60080 (DHAS_VIBMI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aspartate-semialdehyde dehydrogenase
Short name=ASA dehydrogenase
Short name=ASADH
EC=1.2.1.11
Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase
Gene names
Name:asd
OrganismVibrio mimicus
Taxonomic identifier674 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length316 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate By similarity. HAMAP MF_02121

Catalytic activity

L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH. HAMAP MF_02121

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4. HAMAP MF_02121

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. HAMAP MF_02121

Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5. HAMAP MF_02121

Subunit structure

Homodimer By similarity. HAMAP MF_02121

Sequence similarities

Belongs to the aspartate-semialdehyde dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›316›316Aspartate-semialdehyde dehydrogenase HAMAP MF_02121
PRO_0000141395

Regions

Nucleotide binding13 – 164NADP By similarity
Nucleotide binding41 – 422NADP By similarity
Nucleotide binding162 – 1632NADP By similarity

Sites

Active site1321Acyl-thioester intermediate By similarity
Active site2451Proton acceptor By similarity
Binding site1011Phosphate By similarity
Binding site1591Substrate By similarity
Binding site2161Phosphate By similarity
Binding site2381Substrate By similarity
Binding site3161NADP By similarity

Experimental info

Non-terminal residue3161

Sequences

Sequence LengthMass (Da)Tools
Q60080 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5E27AFAD9BC63A11

FASTA31635,139
        10         20         30         40         50         60 
MSQQFNVAIF GATGAVGETM LEVLQEREFP VDELFLLASE RSEGKTYRFN GKTVRVQNVE 

        70         80         90        100        110        120 
EFDWSQVHIA LFSAGGELSA HWAPIRAEAG VVVIDNTSHF RYDYDIPLVI PEVNPEAIAE 

       130        140        150        160        170        180 
FRNRNIIANP NCSTIQMLVA LKPIYDAVGI ERINVTTYQS VSGAGKAGID ELAGQTAKLL 

       190        200        210        220        230        240 
NGYPAETNTF SQQIAFNCIP QIDQFMDNGY TKEEMKMVWE TQKIFNDPSI MVNPTCVRVP 

       250        260        270        280        290        300 
VFYGHAEAVH VETRAPIDAE QVMDMLDQTD GIELFRGADF PTQVRDAGGK DHVLVGRVRN 

       310 
DISHHSGVNL WVVADN

« Hide

References

[1]"The sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-O1 Vibrio cholerae."
Karaolis D.K., Lan R., Reeves P.R.
J. Bacteriol. 177:3191-3198(1995) [PubMed: 7768818] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M547.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U25067 Genomic DNA. Translation: AAC43377.1.

3D structure databases

ProteinModelPortalQ60080.
SMRQ60080. Positions 2-316.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_02121. ASADH.
[Tree]
InterProIPR000319. Asp-semialdehyde_DH_CS.
IPR012080. Asp_semialdehyde_DH.
IPR005986. Asp_semialdehyde_DH_beta.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFPIRSF000148. ASA_dh. 1 hit.
SMARTSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01296. Asd_B. 1 hit.
PROSITEPS01103. ASD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHAS_VIBMI
AccessionPrimary (citable) accession number: Q60080
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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