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Protein

NADH dehydrogenase

Gene

nox

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Can oxidize either NADH or NADPH with a preference for NADH. Can catalyze electron transfer from NADH to various electron acceptors which include, in addition to molecular oxygen, cytochrome c, 2,6 dichlorphenolindophenol, methylene blue, ferricyanide or P-nitroblue tetrazolium.

Catalytic activityi

NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

FMNNote: Binds 1 FMN per subunit.

pH dependencei

Optimum pH is 5.0.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73FMN1 Publication1
Binding sitei195FMN1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 21FMN1 Publication5
Nucleotide bindingi158 – 159FMN1 Publication2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase (EC:1.6.99.3)
Alternative name(s):
H(2)O(2)-forming NADH oxidase
NADH:oxygen oxidoreductase
Gene namesi
Name:nox
Ordered Locus Names:TTHA0425
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000727211 – 205NADH dehydrogenaseAdd BLAST205

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0425.

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 16Combined sources7
Helixi30 – 40Combined sources11
Helixi46 – 48Combined sources3
Beta strandi52 – 57Combined sources6
Helixi60 – 69Combined sources10
Turni70 – 72Combined sources3
Helixi75 – 78Combined sources4
Beta strandi79 – 87Combined sources9
Helixi89 – 94Combined sources6
Helixi95 – 98Combined sources4
Helixi107 – 121Combined sources15
Helixi125 – 149Combined sources25
Beta strandi153 – 157Combined sources5
Helixi162 – 169Combined sources8
Beta strandi176 – 188Combined sources13
Helixi198 – 201Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NOXX-ray1.59A1-205[»]
ProteinModelPortaliQ60049.
SMRiQ60049.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60049.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitroreductase family.Curated

Phylogenomic databases

eggNOGiENOG4108TVJ. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146732.
OMAiTCWIGSF.
PhylomeDBiQ60049.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.

Sequencei

Sequence statusi: Complete.

Q60049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATLPVLDA KTAALKRRSI RRYRKDPVPE GLLREILEAA LRAPSAWNLQ
60 70 80 90 100
PWRIVVVRDP ATKRALREAA FGQAHVEEAP VVLVLYADLE DALAHLDEVI
110 120 130 140 150
HPGVQGERRE AQKQAIQRAF AAMGQEARKA WASGQSYILL GYLLLLLEAY
160 170 180 190 200
GLGSVPMLGF DPERVRAILG LPSHAAIPAL VALGYPAEEG YPSHRLPLER

VVLWR
Length:205
Mass (Da):22,730
Last modified:March 29, 2005 - v2
Checksum:i012742DFA85B50E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174H → R in CAA42707 (PubMed:1577004).Curated1
Sequence conflicti174H → R in AAB25458 (PubMed:1577004).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60110 Genomic DNA. Translation: CAA42707.1.
S55441 Genomic DNA. Translation: AAB25458.1.
AP008226 Genomic DNA. Translation: BAD70248.1.
RefSeqiWP_011227925.1. NC_006461.1.
YP_143691.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70248; BAD70248; BAD70248.
GeneIDi3168975.
KEGGittj:TTHA0425.
PATRICi23955821. VBITheThe93045_0425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60110 Genomic DNA. Translation: CAA42707.1.
S55441 Genomic DNA. Translation: AAB25458.1.
AP008226 Genomic DNA. Translation: BAD70248.1.
RefSeqiWP_011227925.1. NC_006461.1.
YP_143691.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NOXX-ray1.59A1-205[»]
ProteinModelPortaliQ60049.
SMRiQ60049.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70248; BAD70248; BAD70248.
GeneIDi3168975.
KEGGittj:TTHA0425.
PATRICi23955821. VBITheThe93045_0425.

Phylogenomic databases

eggNOGiENOG4108TVJ. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146732.
OMAiTCWIGSF.
PhylomeDBiQ60049.

Miscellaneous databases

EvolutionaryTraceiQ60049.
PROiQ60049.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOX_THET8
AccessioniPrimary (citable) accession number: Q60049
Secondary accession number(s): Q53306, Q5SL68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.