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Protein

Probable cadmium-transporting ATPase

Gene

cadA

Organism
Listeria monocytogenes
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141CadmiumPROSITE-ProRule annotation
Metal bindingi17 – 171CadmiumPROSITE-ProRule annotation
Active sitei398 – 39814-aspartylphosphate intermediateCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiport, Cadmium resistance, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.3. 3045.

Protein family/group databases

TCDBi3.A.3.6.8. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cadmium-transporting ATPase (EC:3.6.3.3)
Alternative name(s):
Cadmium efflux ATPase
Gene namesi
Name:cadA
Encoded oniPlasmid pLm740 Publication
OrganismiListeria monocytogenes
Taxonomic identifieri1639 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence analysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence analysisAdd
BLAST
Transmembranei317 – 33721HelicalSequence analysisAdd
BLAST
Transmembranei347 – 36721HelicalSequence analysisAdd
BLAST
Transmembranei669 – 68921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711Probable cadmium-transporting ATPasePRO_0000046247Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

Secondary structure

1
711
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118Combined sources
Helixi15 – 2713Combined sources
Beta strandi31 – 366Combined sources
Beta strandi39 – 4810Combined sources
Helixi51 – 588Combined sources
Turni59 – 613Combined sources
Beta strandi64 – 663Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJ0NMR-A1-71[»]
2AJ1NMR-A1-71[»]
ProteinModelPortaliQ60048.
SMRiQ60048. Positions 1-71.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60048.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6764HMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK01534.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKTVYRVD GLSCTNCAAK FERNVKEIEG VTEAIVNFGA SKITVTGEAS
60 70 80 90 100
IQQVEQAGAF EHLKIIPEKE SFTDPEHFTD HQSFIRKNWR LLLSGLFIAV
110 120 130 140 150
GYASQIMNGE DFYLTNALFI FAIFIGGYSL FKEGFKNLLK FEFTMETLMT
160 170 180 190 200
IAIIGAAFIG EWAEGSIVVI LFAVSEALER YSMDKARQSI RSLMDIAPKE
210 220 230 240 250
ALVRRSGTDR MVHVDDIQIG DIMIIKPGQK IAMDGHVVKG YSAVNQAAIT
260 270 280 290 300
GESIPVEKNI DDSVFAGTLN EEGLLEVAVT KRVEDTTISK IIHLVEEAQG
310 320 330 340 350
ERAPAQAFVD TFAKYYTPAI IVIAALIATV PPLLFGGNWE TWVYQGLSVL
360 370 380 390 400
VVGCPCALVV STPVAIVTAI GNAAKNGVLV KGGVYLEEIG GLKAIAFDKT
410 420 430 440 450
GTLTKGVPVV TDYIELTEAT NIQHNKNYII MAALEQLSQH PLASAIIKYG
460 470 480 490 500
ETREMDLTSI NVNDFTSITG KGIRGTVDGN TYYVGSPVLF KELLASQFTD
510 520 530 540 550
SIHRQVSDLQ LKGKTAMLFG TNQKLISIVA VADEVRSSSQ HVIKRLHELG
560 570 580 590 600
IEKTIMLTGD NQATAQAIGQ QVGVSEIEGE LMPQDKLDYI KQLKINFGKV
610 620 630 640 650
AMVGDGINDA PALAAATVGI AMGGAGTDTA IETADVALMG DDLQKLPFTV
660 670 680 690 700
KLSRKTLQII KQNITFSLVI KLIALLLVIP GWLTLWIAIM ADMGATLLVT
710
LNGLRLMKVK D
Length:711
Mass (Da):77,089
Last modified:November 1, 1996 - v1
Checksum:iC23BFEB7E20CFE9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28104 Genomic DNA. Translation: AAA25275.1.
RefSeqiWP_003728466.1. NZ_LNNL01000022.1.
YP_003728018.1. NC_014255.1.

Genome annotation databases

GeneIDi9350447.
KEGGipg:9350447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28104 Genomic DNA. Translation: AAA25275.1.
RefSeqiWP_003728466.1. NZ_LNNL01000022.1.
YP_003728018.1. NC_014255.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJ0NMR-A1-71[»]
2AJ1NMR-A1-71[»]
ProteinModelPortaliQ60048.
SMRiQ60048. Positions 1-71.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.3.6.8. the p-type atpase (p-atpase) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9350447.
KEGGipg:9350447.

Phylogenomic databases

KOiK01534.

Enzyme and pathway databases

BRENDAi3.6.3.3. 3045.

Miscellaneous databases

EvolutionaryTraceiQ60048.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Plasmid-borne cadmium resistance genes in Listeria monocytogenes are similar to cadA and cadC of Staphylococcus aureus and are induced by cadmium."
    Lebrun M., Audurier A., Cossart P.
    J. Bacteriol. 176:3040-3048(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LM74.
  2. "Plasmid-borne cadmium resistance genes in Listeria monocytogenes are present on Tn5422, a novel transposon closely related to Tn917."
    Lebrun M., Audurier A., Cossart P.
    J. Bacteriol. 176:3049-3061(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LM74.
    Transposon: Tn5422.

Entry informationi

Entry nameiCADA_LISMN
AccessioniPrimary (citable) accession number: Q60048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.