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Q60042 (XYNA_THENE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,4-beta-xylanase A

Short name=Xylanase A
EC=3.2.1.8
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Endoxylanase
Gene names
Name:xynA
OrganismThermotoga neapolitana
Taxonomic identifier2337 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length1055 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Domain

The C-terminal CBM-CenC domains mediate the binding of XynA to microcrystalline cellulose. CBM-CenC 2 alone can also promote cellulose binding By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Contains 2 CBM-cenC (cenC-type cellulose-binding) domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.5-6.

Temperature dependence:

Optimum temperature is 102 degrees Celsius. Thermostable.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 10551026Endo-1,4-beta-xylanase A
PRO_0000007987

Regions

Domain720 – 851132CBM-cenC 1
Domain895 – 1040146CBM-cenC 2
Region30 – 367338A
Region371 – 687317B, catalytic

Sites

Active site4981Proton donor By similarity
Active site6041Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q60042 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F939845878B87468

FASTA1,055119,323
        10         20         30         40         50         60 
MRKKRRGFLN ASTAVLVGIL AGFLGVVLAA TGALGFAVRE SLLLKQFLFL SFEGNTDGAS 

        70         80         90        100        110        120 
PFGKDVVVTA SQDVAADGEY SLKVENRTSV WDGVEIDLTG KVNTGTDYLL SFHVYQTSDS 

       130        140        150        160        170        180 
PQLFSVLART EDEKGERYKI LADKVVVPNY WKEILVPFSP TFEGTPAKFS LIITSPKKTD 

       190        200        210        220        230        240 
FVFYVDNVQV LTPKEAGPKV VYETSFEKGI GDWQPRGSDV KISISPKVAH SGKKSLFVSN 

       250        260        270        280        290        300 
RQKGWHGAQI SLKGILKTGK TYAFEAWVYQ ESGQDQTIIM TMQRKYSSDS STKYEWIKAA 

       310        320        330        340        350        360 
TVPSGQWVQL SGTYTIPAGV TVEDLTLYFE SQNPTLEFYV DDVKVVDTTS AEIKLEMNPE 

       370        380        390        400        410        420 
EEIPALKDVL KDYFRVGVAL PSKVFINQKD IALISKHSNS STAENEMKPD SLLAGIENGK 

       430        440        450        460        470        480 
LKFRFETADK YIEFAQQNGM VVRGHTLVWH NQTPEWFFKD ENGNLLSKEE MTERLREYIH 

       490        500        510        520        530        540 
TVVGHFKGKV YAWDVVNEAV DPNQPDGLRR STWYQIMGPD YIELAFKFAR EADPNAKLFY 

       550        560        570        580        590        600 
NDYNTFEPKK RDIIYNLVKS LKEKGLIDGI GMQCHISLAT DIRQIEEAIK KFSTIPGIEI 

       610        620        630        640        650        660 
HITELDISVY RDSTSNYSEA PRTALIEQAH KMAQLFKIFK KYSNVITNVT FWGLKDDYSW 

       670        680        690        700        710        720 
RATRRNDWPL IFDKDYQAKL AYWAIVAPEV LPPLPKESKI SEGEAVVVGM MDDSYMMSKP 

       730        740        750        760        770        780 
IEIYDEEGNV KATIRAIWKD STIYVYGEVQ DATKKPAEDG VAIFINPNNE RTPYLQPDDT 

       790        800        810        820        830        840 
YVVLWTNWKS EVNREDVEVK KFVGPGFRRY SFEMSITIPG VEFKKDSYIG FDVAVIDDGK 

       850        860        870        880        890        900 
WYSWSDTTNS QKTNTMNYGT LKLEGVMVAT AKYGTPVIDG EIDDIWNTTE EIETKSVAMG 

       910        920        930        940        950        960 
SLEKNATAKV RVLWDEENLY VLAIVKDPVL NKDNSNPWEQ DSVEIFIDEN NHKTGYYEDD 

       970        980        990       1000       1010       1020 
DAQFRVNYMN EQSFGTGASA ARFKTAVKLI EGGYIVEAAI KWKTIKPSPN TVIGFNVQVN 

      1030       1040       1050 
DANEKGQRVG IISWSDPTNN SWRDPSKFGN LRLIK 

« Hide

References

[1]"The multidomain xylanase A of the hyperthermophilic bacterium Thermotoga neapolitana is extremely thermoresistant."
Zverlov V., Piotukh K., Dakhova O., Velikodvorskaya G., Borriss R.
Appl. Microbiol. Biotechnol. 45:245-247(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Z2706-MC24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46945 Genomic DNA. Translation: CAA87069.1.

3D structure databases

ProteinModelPortalQ60042.
SMRQ60042. Positions 867-1055.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Proteomic databases

PRIDEQ60042.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProIPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR006311. TAT_signal.
[Graphical view]
PfamPF02018. CBM_4_9. 2 hits.
PF06452. DUF1083. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSPR00134. GLHYDRLASE10.
SMARTSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXYNA_THENE
AccessionPrimary (citable) accession number: Q60042
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries