Q60042 (XYNA_THENE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,4-beta-xylanase A
Short name=Xylanase A
EC=3.2.1.8

Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Endoxylanase

Gene names

Name:

xynA

Organism

Thermotoga neapolitana

Taxonomic identifier

2337 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	1055 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Domain	The C-terminal CBM-CenC domains mediate the binding of XynA to microcrystalline cellulose. CBM-CenC 2 alone can also promote cellulose binding By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family. Contains 2 CBM-cenC (cenC-type cellulose-binding) domains.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5-6. Temperature dependence: Optimum temperature is 102 degrees Celsius. Thermostable.

Ontologies

Keywords
Biological process	Xylan degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 1055	1026	Endo-1,4-beta-xylanase A		PRO_0000007987
Regions
	Domain	720 – 851	132	CBM-cenC 1
	Domain	895 – 1040	146	CBM-cenC 2
	Region	30 – 367	338	A
	Region	371 – 687	317	B, catalytic
Sites
	Active site	498	1	Proton donor By similarity
	Active site	604	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60042 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F939845878B87468	FASTA	1,055	119,323
	10 20 30 40 50 60 MRKKRRGFLN ASTAVLVGIL AGFLGVVLAA TGALGFAVRE SLLLKQFLFL SFEGNTDGAS 70 80 90 100 110 120 PFGKDVVVTA SQDVAADGEY SLKVENRTSV WDGVEIDLTG KVNTGTDYLL SFHVYQTSDS 130 140 150 160 170 180 PQLFSVLART EDEKGERYKI LADKVVVPNY WKEILVPFSP TFEGTPAKFS LIITSPKKTD 190 200 210 220 230 240 FVFYVDNVQV LTPKEAGPKV VYETSFEKGI GDWQPRGSDV KISISPKVAH SGKKSLFVSN 250 260 270 280 290 300 RQKGWHGAQI SLKGILKTGK TYAFEAWVYQ ESGQDQTIIM TMQRKYSSDS STKYEWIKAA 310 320 330 340 350 360 TVPSGQWVQL SGTYTIPAGV TVEDLTLYFE SQNPTLEFYV DDVKVVDTTS AEIKLEMNPE 370 380 390 400 410 420 EEIPALKDVL KDYFRVGVAL PSKVFINQKD IALISKHSNS STAENEMKPD SLLAGIENGK 430 440 450 460 470 480 LKFRFETADK YIEFAQQNGM VVRGHTLVWH NQTPEWFFKD ENGNLLSKEE MTERLREYIH 490 500 510 520 530 540 TVVGHFKGKV YAWDVVNEAV DPNQPDGLRR STWYQIMGPD YIELAFKFAR EADPNAKLFY 550 560 570 580 590 600 NDYNTFEPKK RDIIYNLVKS LKEKGLIDGI GMQCHISLAT DIRQIEEAIK KFSTIPGIEI 610 620 630 640 650 660 HITELDISVY RDSTSNYSEA PRTALIEQAH KMAQLFKIFK KYSNVITNVT FWGLKDDYSW 670 680 690 700 710 720 RATRRNDWPL IFDKDYQAKL AYWAIVAPEV LPPLPKESKI SEGEAVVVGM MDDSYMMSKP 730 740 750 760 770 780 IEIYDEEGNV KATIRAIWKD STIYVYGEVQ DATKKPAEDG VAIFINPNNE RTPYLQPDDT 790 800 810 820 830 840 YVVLWTNWKS EVNREDVEVK KFVGPGFRRY SFEMSITIPG VEFKKDSYIG FDVAVIDDGK 850 860 870 880 890 900 WYSWSDTTNS QKTNTMNYGT LKLEGVMVAT AKYGTPVIDG EIDDIWNTTE EIETKSVAMG 910 920 930 940 950 960 SLEKNATAKV RVLWDEENLY VLAIVKDPVL NKDNSNPWEQ DSVEIFIDEN NHKTGYYEDD 970 980 990 1000 1010 1020 DAQFRVNYMN EQSFGTGASA ARFKTAVKLI EGGYIVEAAI KWKTIKPSPN TVIGFNVQVN 1030 1040 1050 DANEKGQRVG IISWSDPTNN SWRDPSKFGN LRLIK

References

[1]

"The multidomain xylanase A of the hyperthermophilic bacterium Thermotoga neapolitana is extremely thermoresistant." Zverlov V., Piotukh K., Dakhova O., Velikodvorskaya G., Borriss R. Appl. Microbiol. Biotechnol. 45:245-247(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Z2706-MC24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46945 Genomic DNA. Translation: CAA87069.1.
3D structure databases
ProteinModelPortal	Q60042.
SMR	Q60042. Positions 867-1055.
ModBase	Search...
Protein family/group databases
CAZy	CBM22. Carbohydrate-Binding Module Family 22. CBM9. Carbohydrate-Binding Module Family 9. GH10. Glycoside Hydrolase Family 10.
Proteomic databases
PRIDE	Q60042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.260. 2 hits. 2.60.40.1190. 2 hits. 3.20.20.80. 1 hit.
InterPro	IPR010502. Carb-bd_dom_fam9. IPR008960. Carb-bd_dom_fam9-like. IPR015922. Carb-bd_dom_fam9-like_subgr. IPR003305. CenC_carb-bd. IPR008979. Galactose-bd-like. IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR006311. TAT_signal. [Graphical view]
Pfam	PF02018. CBM_4_9. 2 hits. PF06452. DUF1083. 2 hits. PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
SUPFAM	SSF49344. CBD9-like. 2 hits. SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. 1 hit. PS51318. TAT. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_THENE

Accession

Primary (citable) accession number: Q60042

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program