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Q60037 (XYNA_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,4-beta-xylanase A

Short name=Xylanase A
EC=3.2.1.8
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene names
Name:xynA
Ordered Locus Names:TM_0061
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length1059 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Domain

The C-terminal CBM-CenC domains mediate the binding of XynA to microcrystalline cellulose. CBM-CenC 2 alone can also promote cellulose binding.

Sequence similarities

Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Contains 2 CBM-cenC (cenC-type cellulose-binding) domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 6.2.

Temperature dependence:

Optimum temperature is 90 degrees Celsius. Thermostable.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 10591029Endo-1,4-beta-xylanase A
PRO_0000007986

Regions

Domain700 – 870171CBM-cenC 1
Domain871 – 1059189CBM-cenC 2
Region47 – 199153A-1
Region200 – 354155A-2
Region355 – 691337B, catalytic

Sites

Active site5021Proton donor By similarity
Active site6081Nucleophile By similarity

Secondary structure

......................................... 1059
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q60037 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 045936844A2D72E2

FASTA1,059119,643
        10         20         30         40         50         60 
MQVRKRRGLL DVSTAVLVGI LAGFLGVVLA ASGVLSFGKE ASSKGDSSLE TVLALSFEGT 

        70         80         90        100        110        120 
TEGVVPFGKD VVLTASQDVA ADGEYSLKVE NRTSPWDGVE IDLTGKVKSG ADYLLSFQVY 

       130        140        150        160        170        180 
QSSDAPQLFN VVARTEDEKG ERYDVILDKV VVSDHWKEIL VPFSPTFEGT PAKYSLIIVA 

       190        200        210        220        230        240 
SKNTNFNFYL DKVQVLAPKE SGPKVIYETS FENGVGDWQP RGDVNIEASS EVAHSGKSSL 

       250        260        270        280        290        300 
FISNRQKGWQ GAQINLKGIL KTGKTYAFEA WVYQNSGQDQ TIIMTMQRKY SSDASTQYEW 

       310        320        330        340        350        360 
IKSATVPSGQ WVQLSGTYTI PAGVTVEDLT LYFESQNPTL EFYVDDVKIV DTTSAEIKIE 

       370        380        390        400        410        420 
MEPEKEIPAL KEVLKDYFKV GVALPSKVFL NPKDIELITK HFNSITAENE MKPESLLAGI 

       430        440        450        460        470        480 
ENGKLKFRFE TADKYIQFVE ENGMVIRGHT LVWHNQTPDW FFKDENGNLL SKEAMTERLK 

       490        500        510        520        530        540 
EYIHTVVGHF KGKVYAWDVV NEAVDPNQPD GLRRSTWYQI MGPDYIELAF KFAREADPDA 

       550        560        570        580        590        600 
KLFYNDYNTF EPRKRDIIYN LVKDLKEKGL IDGIGMQCHI SLATDIKQIE EAIKKFSTIP 

       610        620        630        640        650        660 
GIEIHITELD MSVYRDSSSN YPEAPRTALI EQAHKMMQLF EIFKKYSNVI TNVTFWGLKD 

       670        680        690        700        710        720 
DYSWRATRRN DWPLIFDKDH QAKLAYWAIV APEVLPPLPK ESRISEGEAV VVGMMDDSYL 

       730        740        750        760        770        780 
MSKPIEILDE EGNVKATIRA VWKDSTIYIY GEVQDKTKKP AEDGVAIFIN PNNERTPYLQ 

       790        800        810        820        830        840 
PDDTYAVLWT NWKTEVNRED VQVKKFVGPG FRRYSFEMSI TIPGVEFKKD SYIGFDAAVI 

       850        860        870        880        890        900 
DDGKWYSWSD TTNSQKTNTM NYGTLKLEGI MVATAKYGTP VIDGEIDEIW NTTEEIETKA 

       910        920        930        940        950        960 
VAMGSLDKNA TAKVRVLWDE NYLYVLAIVK DPVLNKDNSN PWEQDSVEIF IDENNHKTGY 

       970        980        990       1000       1010       1020 
YEDDDAQFRV NYMNEQTFGT GGSPARFKTA VKLIEGGYIV EAAIKWKTIK PTPNTVIGFN 

      1030       1040       1050 
IQVNDANEKG QRVGIISWSD PTNNSWRDPS KFGNLRLIK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacterium Thermotoga maritima."
Winterhalter C., Heinrich P., Candussio A., Wich G., Liebl W.
Mol. Microbiol. 15:431-444(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46264 Genomic DNA. Translation: CAA86406.1.
AE000512 Genomic DNA. Translation: AAD35155.1.
PIRS61311.
RefSeqNP_227877.1. NC_000853.1.
YP_007976409.1. NC_021214.1.
YP_008990963.1. NC_023151.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1I82X-ray1.90A871-1059[»]
1I8AX-ray1.90A871-1059[»]
1I8UX-ray1.90A871-1059[»]
ProteinModelPortalQ60037.
SMRQ60037. Positions 871-1059.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0061.

Protein family/group databases

CAZyCBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Proteomic databases

PRIDEQ60037.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35155; AAD35155; TM_0061.
GeneID15493662.
18092750.
896885.
KEGGtma:TM0061.
tmm:Tmari_0058.
PATRIC23934962. VBITheMar51294_0059.

Phylogenomic databases

eggNOGCOG3693.
KOK01181.
OMAAKLAYWA.
OrthoDBEOG6B363F.
ProtClustDBCLSK874945.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16897.
BRENDA3.2.1.8. 6331.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProIPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02018. CBM_4_9. 2 hits.
PF06452. DUF1083. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSPR00134. GLHYDRLASE10.
SMARTSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS00591. GLYCOSYL_HYDROL_F10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ60037.

Entry information

Entry nameXYNA_THEMA
AccessionPrimary (citable) accession number: Q60037
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries