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Protein

Endo-1,4-beta-xylanase A

Gene

xynA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 6.2.

Temperature dependencei

Optimum temperature is 90 degrees Celsius. Thermostable.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei502Proton donorBy similarity1
Active sitei608NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16897.
BRENDAi3.2.1.8. 6331.

Protein family/group databases

CAZyiCBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
Ordered Locus Names:TM_0061
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000798631 – 1059Endo-1,4-beta-xylanase AAdd BLAST1029

Proteomic databases

PRIDEiQ60037.

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0061.

Structurei

Secondary structure

11059
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi872 – 877Combined sources6
Beta strandi882 – 885Combined sources4
Helixi888 – 892Combined sources5
Beta strandi895 – 897Combined sources3
Beta strandi900 – 904Combined sources5
Turni906 – 908Combined sources3
Beta strandi911 – 918Combined sources8
Beta strandi920 – 930Combined sources11
Beta strandi938 – 940Combined sources3
Helixi941 – 943Combined sources3
Beta strandi944 – 952Combined sources9
Beta strandi965 – 971Combined sources7
Beta strandi976 – 978Combined sources3
Helixi984 – 986Combined sources3
Beta strandi987 – 994Combined sources8
Beta strandi997 – 1005Combined sources9
Beta strandi1015 – 1026Combined sources12
Beta strandi1032 – 1039Combined sources8
Beta strandi1041 – 1043Combined sources3
Turni1045 – 1047Combined sources3
Helixi1049 – 1051Combined sources3
Beta strandi1052 – 1058Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I82X-ray1.90A871-1059[»]
1I8AX-ray1.90A871-1059[»]
1I8UX-ray1.90A871-1059[»]
ProteinModelPortaliQ60037.
SMRiQ60037.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60037.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini364 – 692GH10PROSITE-ProRule annotationAdd BLAST329
Domaini700 – 870CBM-cenC 1Add BLAST171
Domaini871 – 1059CBM-cenC 2Add BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 199A-1Add BLAST153
Regioni200 – 354A-2Add BLAST155

Domaini

The C-terminal CBM-CenC domains mediate the binding of XynA to microcrystalline cellulose. CBM-CenC 2 alone can also promote cellulose binding.

Sequence similaritiesi

Contains 1 GH10 (glycosyl hydrolase family 10) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105D9F. Bacteria.
COG3693. LUCA.
InParanoidiQ60037.
KOiK01181.
OMAiIGMQMHI.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06452. CBM9_1. 2 hits.
PF02018. CBM_4_9. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVRKRRGLL DVSTAVLVGI LAGFLGVVLA ASGVLSFGKE ASSKGDSSLE
60 70 80 90 100
TVLALSFEGT TEGVVPFGKD VVLTASQDVA ADGEYSLKVE NRTSPWDGVE
110 120 130 140 150
IDLTGKVKSG ADYLLSFQVY QSSDAPQLFN VVARTEDEKG ERYDVILDKV
160 170 180 190 200
VVSDHWKEIL VPFSPTFEGT PAKYSLIIVA SKNTNFNFYL DKVQVLAPKE
210 220 230 240 250
SGPKVIYETS FENGVGDWQP RGDVNIEASS EVAHSGKSSL FISNRQKGWQ
260 270 280 290 300
GAQINLKGIL KTGKTYAFEA WVYQNSGQDQ TIIMTMQRKY SSDASTQYEW
310 320 330 340 350
IKSATVPSGQ WVQLSGTYTI PAGVTVEDLT LYFESQNPTL EFYVDDVKIV
360 370 380 390 400
DTTSAEIKIE MEPEKEIPAL KEVLKDYFKV GVALPSKVFL NPKDIELITK
410 420 430 440 450
HFNSITAENE MKPESLLAGI ENGKLKFRFE TADKYIQFVE ENGMVIRGHT
460 470 480 490 500
LVWHNQTPDW FFKDENGNLL SKEAMTERLK EYIHTVVGHF KGKVYAWDVV
510 520 530 540 550
NEAVDPNQPD GLRRSTWYQI MGPDYIELAF KFAREADPDA KLFYNDYNTF
560 570 580 590 600
EPRKRDIIYN LVKDLKEKGL IDGIGMQCHI SLATDIKQIE EAIKKFSTIP
610 620 630 640 650
GIEIHITELD MSVYRDSSSN YPEAPRTALI EQAHKMMQLF EIFKKYSNVI
660 670 680 690 700
TNVTFWGLKD DYSWRATRRN DWPLIFDKDH QAKLAYWAIV APEVLPPLPK
710 720 730 740 750
ESRISEGEAV VVGMMDDSYL MSKPIEILDE EGNVKATIRA VWKDSTIYIY
760 770 780 790 800
GEVQDKTKKP AEDGVAIFIN PNNERTPYLQ PDDTYAVLWT NWKTEVNRED
810 820 830 840 850
VQVKKFVGPG FRRYSFEMSI TIPGVEFKKD SYIGFDAAVI DDGKWYSWSD
860 870 880 890 900
TTNSQKTNTM NYGTLKLEGI MVATAKYGTP VIDGEIDEIW NTTEEIETKA
910 920 930 940 950
VAMGSLDKNA TAKVRVLWDE NYLYVLAIVK DPVLNKDNSN PWEQDSVEIF
960 970 980 990 1000
IDENNHKTGY YEDDDAQFRV NYMNEQTFGT GGSPARFKTA VKLIEGGYIV
1010 1020 1030 1040 1050
EAAIKWKTIK PTPNTVIGFN IQVNDANEKG QRVGIISWSD PTNNSWRDPS

KFGNLRLIK
Length:1,059
Mass (Da):119,643
Last modified:November 1, 1997 - v1
Checksum:i045936844A2D72E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46264 Genomic DNA. Translation: CAA86406.1.
AE000512 Genomic DNA. Translation: AAD35155.1.
PIRiS61311.
RefSeqiNP_227877.1. NC_000853.1.
WP_004082550.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35155; AAD35155; TM_0061.
GeneIDi896885.
KEGGitma:TM0061.
PATRICi23934962. VBITheMar51294_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46264 Genomic DNA. Translation: CAA86406.1.
AE000512 Genomic DNA. Translation: AAD35155.1.
PIRiS61311.
RefSeqiNP_227877.1. NC_000853.1.
WP_004082550.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I82X-ray1.90A871-1059[»]
1I8AX-ray1.90A871-1059[»]
1I8UX-ray1.90A871-1059[»]
ProteinModelPortaliQ60037.
SMRiQ60037.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0061.

Protein family/group databases

CAZyiCBM22. Carbohydrate-Binding Module Family 22.
CBM9. Carbohydrate-Binding Module Family 9.
GH10. Glycoside Hydrolase Family 10.

Proteomic databases

PRIDEiQ60037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35155; AAD35155; TM_0061.
GeneIDi896885.
KEGGitma:TM0061.
PATRICi23934962. VBITheMar51294_0059.

Phylogenomic databases

eggNOGiENOG4105D9F. Bacteria.
COG3693. LUCA.
InParanoidiQ60037.
KOiK01181.
OMAiIGMQMHI.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16897.
BRENDAi3.2.1.8. 6331.

Miscellaneous databases

EvolutionaryTraceiQ60037.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.1190. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR010502. Carb-bd_dom_fam9.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06452. CBM9_1. 2 hits.
PF02018. CBM_4_9. 2 hits.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYNA_THEMA
AccessioniPrimary (citable) accession number: Q60037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.